Endophytic non-pathogenic Fusarium oxysporum-derived dual benefit for nematode management and improved banana (Musa spp.) productivit

The banana weevil (Cosmopolites sordidus) and the burrowing nematode Radopholus similis represent two of the most important pests of bananas. Previously, colonization of banana plants by the non-pathogenic Fusarium oxysporum (isolate V5w2) and the entomopathogenic Beauveria bassiana (isolate WA) hav...

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Main Authors: Kisaakye, J., Fourie, H., Haukeland, Solveig, Kisitu, J., Nakimera, S., Cortada Gonzales, L., Subramanian, S., Coyne, D.
Format: Journal Article
Language:Inglés
Published: MDPI 2022
Subjects:
Online Access:https://hdl.handle.net/10568/118218
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author Kisaakye, J.
Fourie, H.
Haukeland, Solveig
Kisitu, J.
Nakimera, S.
Cortada Gonzales, L.
Subramanian, S.
Coyne, D.
author_browse Cortada Gonzales, L.
Coyne, D.
Fourie, H.
Haukeland, Solveig
Kisaakye, J.
Kisitu, J.
Nakimera, S.
Subramanian, S.
author_facet Kisaakye, J.
Fourie, H.
Haukeland, Solveig
Kisitu, J.
Nakimera, S.
Cortada Gonzales, L.
Subramanian, S.
Coyne, D.
author_sort Kisaakye, J.
collection Repository of Agricultural Research Outputs (CGSpace)
description The banana weevil (Cosmopolites sordidus) and the burrowing nematode Radopholus similis represent two of the most important pests of bananas. Previously, colonization of banana plants by the non-pathogenic Fusarium oxysporum (isolate V5w2) and the entomopathogenic Beauveria bassiana (isolate WA) have been shown to increase host resistance to various banana pests and diseases. However, there is limited data on how the combined inoculation of these isolates would affect field performance of bananas. In this study, the fungal endophytes were inoculated separately and in combination. Tissue cultured plantlets of cooking banana cultivar Mbwazirume and dessert banana cultivar Grande Naine were inoculated by root drenching with a suspension of 1.0 × 107 spores mL−1 of the endophytes on three occasions, separated 4 weeks apart, before transplanting into the field. Each plantlet was further inoculated with 1800 nematodes, composed primarily of R. similis. Inoculation of banana plants with the fungal endophytes significantly reduced nematode densities by >34%. Similarly, plant toppling was lower in the endophyte-enhanced plants (<16.5%) compared with the control (23.3%). We also observed improved yield of the first crop cycle in the endophyte-enhanced plants, which yielded >11 t ha−1 year−1 versus 9 t ha−1 year−1 achieved in the non-inoculated plants. These findings demonstrate the benefits of fungal endophytes in improving the yield of both cooking and dessert bananas via suppression of nematode densities and nematode-related damage
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spelling CGSpace1182182025-12-08T10:29:22Z Endophytic non-pathogenic Fusarium oxysporum-derived dual benefit for nematode management and improved banana (Musa spp.) productivit Kisaakye, J. Fourie, H. Haukeland, Solveig Kisitu, J. Nakimera, S. Cortada Gonzales, L. Subramanian, S. Coyne, D. bananas nematodes biological control endophytes antagonists cosmopolites sordidus The banana weevil (Cosmopolites sordidus) and the burrowing nematode Radopholus similis represent two of the most important pests of bananas. Previously, colonization of banana plants by the non-pathogenic Fusarium oxysporum (isolate V5w2) and the entomopathogenic Beauveria bassiana (isolate WA) have been shown to increase host resistance to various banana pests and diseases. However, there is limited data on how the combined inoculation of these isolates would affect field performance of bananas. In this study, the fungal endophytes were inoculated separately and in combination. Tissue cultured plantlets of cooking banana cultivar Mbwazirume and dessert banana cultivar Grande Naine were inoculated by root drenching with a suspension of 1.0 × 107 spores mL−1 of the endophytes on three occasions, separated 4 weeks apart, before transplanting into the field. Each plantlet was further inoculated with 1800 nematodes, composed primarily of R. similis. Inoculation of banana plants with the fungal endophytes significantly reduced nematode densities by >34%. Similarly, plant toppling was lower in the endophyte-enhanced plants (<16.5%) compared with the control (23.3%). We also observed improved yield of the first crop cycle in the endophyte-enhanced plants, which yielded >11 t ha−1 year−1 versus 9 t ha−1 year−1 achieved in the non-inoculated plants. These findings demonstrate the benefits of fungal endophytes in improving the yield of both cooking and dessert bananas via suppression of nematode densities and nematode-related damage 2022-01-18 2022-02-22T14:02:31Z 2022-02-22T14:02:31Z Journal Article https://hdl.handle.net/10568/118218 en Open Access application/pdf MDPI Kisaakye, J., Fourie, H., Haukeland, S., Kisitu, J., Nakimera, S., Cortada, L., ... & Coyne, D. (2022). Endophytic non-pathogenic Fusarium oxysporum-derived dual benefit for nematode management and improved banana (Musa spp.) productivit. Agriculture, 12(2):125, 1-17.
spellingShingle bananas
nematodes
biological control
endophytes
antagonists
cosmopolites sordidus
Kisaakye, J.
Fourie, H.
Haukeland, Solveig
Kisitu, J.
Nakimera, S.
Cortada Gonzales, L.
Subramanian, S.
Coyne, D.
Endophytic non-pathogenic Fusarium oxysporum-derived dual benefit for nematode management and improved banana (Musa spp.) productivit
title Endophytic non-pathogenic Fusarium oxysporum-derived dual benefit for nematode management and improved banana (Musa spp.) productivit
title_full Endophytic non-pathogenic Fusarium oxysporum-derived dual benefit for nematode management and improved banana (Musa spp.) productivit
title_fullStr Endophytic non-pathogenic Fusarium oxysporum-derived dual benefit for nematode management and improved banana (Musa spp.) productivit
title_full_unstemmed Endophytic non-pathogenic Fusarium oxysporum-derived dual benefit for nematode management and improved banana (Musa spp.) productivit
title_short Endophytic non-pathogenic Fusarium oxysporum-derived dual benefit for nematode management and improved banana (Musa spp.) productivit
title_sort endophytic non pathogenic fusarium oxysporum derived dual benefit for nematode management and improved banana musa spp productivit
topic bananas
nematodes
biological control
endophytes
antagonists
cosmopolites sordidus
url https://hdl.handle.net/10568/118218
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