Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay
The fall armyworm [FAW, Spodoptera frugiperda (J E Smith)], a moth native to America, has spread throughout the world since it was first discovered in Africa in 2016. The FAW is a polyphagous migratory pest that can travel over long distances using seasonal winds or typhoons because of its excellent...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2022
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/118216 |
| _version_ | 1855513110378446848 |
|---|---|
| author | Osabutey, A.F. Seo, B.Y. Kim, A.Y. Ha, T.A.T. Jung, J. Goergen, Georg E. Owusu, E.O. Lee, G.S. Koh, Y.H. |
| author_browse | Goergen, Georg E. Ha, T.A.T. Jung, J. Kim, A.Y. Koh, Y.H. Lee, G.S. Osabutey, A.F. Owusu, E.O. Seo, B.Y. |
| author_facet | Osabutey, A.F. Seo, B.Y. Kim, A.Y. Ha, T.A.T. Jung, J. Goergen, Georg E. Owusu, E.O. Lee, G.S. Koh, Y.H. |
| author_sort | Osabutey, A.F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The fall armyworm [FAW, Spodoptera frugiperda (J E Smith)], a moth native to America, has spread throughout the world since it was first discovered in Africa in 2016. The FAW is a polyphagous migratory pest that can travel over long distances using seasonal winds or typhoons because of its excellent flying ability, causing serious damage to many crops. For effective FAW control, accurate species identification is essential at the beginning of the invasion. In this study, the FAW-specific gene Sf00067 was discovered by performing bioinformatics to develop a fast and accurate tool for the species-specific diagnosis of this pest. An Sf00067 loop-mediated isothermal amplification (LAMP) assay was developed, and optimal conditions were established. The Sf00067 6 primer LAMP (Sf6p-LAMP) assay established in this study was able to diagnose various genotype-based strains of FAW captured in Korea and FAWs collected from Benin, Africa. Our FAW diagnostic protocol can be completed within 30 min, from the process of extracting genomic DNA from an egg or a 1st instar larva to species determination. |
| format | Journal Article |
| id | CGSpace118216 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1182162025-12-08T09:54:28Z Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay Osabutey, A.F. Seo, B.Y. Kim, A.Y. Ha, T.A.T. Jung, J. Goergen, Georg E. Owusu, E.O. Lee, G.S. Koh, Y.H. spodoptera frugiperda genes dna pests of plants maize pest control The fall armyworm [FAW, Spodoptera frugiperda (J E Smith)], a moth native to America, has spread throughout the world since it was first discovered in Africa in 2016. The FAW is a polyphagous migratory pest that can travel over long distances using seasonal winds or typhoons because of its excellent flying ability, causing serious damage to many crops. For effective FAW control, accurate species identification is essential at the beginning of the invasion. In this study, the FAW-specific gene Sf00067 was discovered by performing bioinformatics to develop a fast and accurate tool for the species-specific diagnosis of this pest. An Sf00067 loop-mediated isothermal amplification (LAMP) assay was developed, and optimal conditions were established. The Sf00067 6 primer LAMP (Sf6p-LAMP) assay established in this study was able to diagnose various genotype-based strains of FAW captured in Korea and FAWs collected from Benin, Africa. Our FAW diagnostic protocol can be completed within 30 min, from the process of extracting genomic DNA from an egg or a 1st instar larva to species determination. 2022 2022-02-22T10:52:30Z 2022-02-22T10:52:30Z Journal Article https://hdl.handle.net/10568/118216 en Open Access application/pdf Springer Osabutey, A.F., Seo, B.Y., Kim, A.Y., Ha, T.A.T., Jung, J., Goergen, G., ... & Koh, Y.H. (2022). Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay. Scientific Reports, 12(1), 1-10. |
| spellingShingle | spodoptera frugiperda genes dna pests of plants maize pest control Osabutey, A.F. Seo, B.Y. Kim, A.Y. Ha, T.A.T. Jung, J. Goergen, Georg E. Owusu, E.O. Lee, G.S. Koh, Y.H. Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay |
| title | Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay |
| title_full | Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay |
| title_fullStr | Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay |
| title_full_unstemmed | Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay |
| title_short | Identification of a fall armyworm (Spodoptera frugiperda)-specific gene and development of a rapid and sensitive loop-mediated isothermal amplification assay |
| title_sort | identification of a fall armyworm spodoptera frugiperda specific gene and development of a rapid and sensitive loop mediated isothermal amplification assay |
| topic | spodoptera frugiperda genes dna pests of plants maize pest control |
| url | https://hdl.handle.net/10568/118216 |
| work_keys_str_mv | AT osabuteyaf identificationofafallarmywormspodopterafrugiperdaspecificgeneanddevelopmentofarapidandsensitiveloopmediatedisothermalamplificationassay AT seoby identificationofafallarmywormspodopterafrugiperdaspecificgeneanddevelopmentofarapidandsensitiveloopmediatedisothermalamplificationassay AT kimay identificationofafallarmywormspodopterafrugiperdaspecificgeneanddevelopmentofarapidandsensitiveloopmediatedisothermalamplificationassay AT hatat identificationofafallarmywormspodopterafrugiperdaspecificgeneanddevelopmentofarapidandsensitiveloopmediatedisothermalamplificationassay AT jungj identificationofafallarmywormspodopterafrugiperdaspecificgeneanddevelopmentofarapidandsensitiveloopmediatedisothermalamplificationassay AT goergengeorge identificationofafallarmywormspodopterafrugiperdaspecificgeneanddevelopmentofarapidandsensitiveloopmediatedisothermalamplificationassay AT owusueo identificationofafallarmywormspodopterafrugiperdaspecificgeneanddevelopmentofarapidandsensitiveloopmediatedisothermalamplificationassay AT leegs identificationofafallarmywormspodopterafrugiperdaspecificgeneanddevelopmentofarapidandsensitiveloopmediatedisothermalamplificationassay AT kohyh identificationofafallarmywormspodopterafrugiperdaspecificgeneanddevelopmentofarapidandsensitiveloopmediatedisothermalamplificationassay |