Drying banana seeds for ex situ conservation
The ability of seeds to withstand drying is fundamental to ex situ seed conservation but drying responses are not well known for most wild species including crop wild relatives. We look at drying responses of seeds of Musa acuminata and Musa balbisiana, the two primary wild relatives of bananas and...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Oxford University Press
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/117591 |
| _version_ | 1855521354414030848 |
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| author | Kallow, Simon Garcia Zuluaga, Manuela Fanega Sleziak, Natalia Nugraha, Bayu Mertens, Arne Janssens, Steven B. Gueco, Lavernee Valle-Descalsota, Michelle Lyka Vu, Tuong Dang Vu, Dang Toan Li, Loan Thi Vandelook, Filip Dickie, John B. Verboven, Peter Swennen, Rony L. Panis, Bartholomeus |
| author_browse | Dickie, John B. Fanega Sleziak, Natalia Garcia Zuluaga, Manuela Gueco, Lavernee Janssens, Steven B. Kallow, Simon Li, Loan Thi Mertens, Arne Nugraha, Bayu Panis, Bartholomeus Swennen, Rony L. Valle-Descalsota, Michelle Lyka Vandelook, Filip Verboven, Peter Vu, Dang Toan Vu, Tuong Dang |
| author_facet | Kallow, Simon Garcia Zuluaga, Manuela Fanega Sleziak, Natalia Nugraha, Bayu Mertens, Arne Janssens, Steven B. Gueco, Lavernee Valle-Descalsota, Michelle Lyka Vu, Tuong Dang Vu, Dang Toan Li, Loan Thi Vandelook, Filip Dickie, John B. Verboven, Peter Swennen, Rony L. Panis, Bartholomeus |
| author_sort | Kallow, Simon |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The ability of seeds to withstand drying is fundamental to ex situ seed conservation but drying responses are not well known for most wild species including crop wild relatives. We look at drying responses of seeds of Musa acuminata and Musa balbisiana, the two primary wild relatives of bananas and plantains, using the following four experimental approaches: (i)We equilibrated seeds to a range of relative humidity (RH) levels using non-saturated lithium chloride solutions and subsequently measured moisture content (MC) and viability. At each humidity levelwe tested viability using embryo rescue (ER), tetrazolium chloride staining and germination in an incubator.We found that seed viabilitywas not reduced when seedswere dried to 4% equilibrium relative humidity (eRH; equating to 2.5% MC). (ii)We assessed viability ofmature and less mature seeds using ER and germination in the soil and tested responses to drying. Findings showed that seeds must be fully mature to germinate and immature seeds had negligible viability. (iii) We dried seeds extracted from ripe/unripe fruit to 35–40% eRH at different rates and tested viability with germination tests in the soil. Seeds from unripe fruit lost viability when dried and especially when dried faster; seeds from ripe fruit only lost viability when fast dried. (iv) Finally, we dried and re-imbibed mature and
less mature seeds and measured embryo shrinkage and volume change using X-ray computer tomography. Embryos of less mature seeds shrank significantly when dried to 15% eRH from 0.468 to 0.262 mm3, but embryos of mature seeds did not.
Based on our results, mature seeds from ripe fruit are desiccation tolerant to moisture levels required for seed genebanking but embryos from immature seeds are mechanistically less able to withstand desiccation, especially when water potential gradients are high. |
| format | Journal Article |
| id | CGSpace117591 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1175912025-11-11T17:46:45Z Drying banana seeds for ex situ conservation Kallow, Simon Garcia Zuluaga, Manuela Fanega Sleziak, Natalia Nugraha, Bayu Mertens, Arne Janssens, Steven B. Gueco, Lavernee Valle-Descalsota, Michelle Lyka Vu, Tuong Dang Vu, Dang Toan Li, Loan Thi Vandelook, Filip Dickie, John B. Verboven, Peter Swennen, Rony L. Panis, Bartholomeus crop wild relatives drought stress genetic resources gene banks especies silvestres afín a las plantas cultivadas estrés de sequia recursos genéticos The ability of seeds to withstand drying is fundamental to ex situ seed conservation but drying responses are not well known for most wild species including crop wild relatives. We look at drying responses of seeds of Musa acuminata and Musa balbisiana, the two primary wild relatives of bananas and plantains, using the following four experimental approaches: (i)We equilibrated seeds to a range of relative humidity (RH) levels using non-saturated lithium chloride solutions and subsequently measured moisture content (MC) and viability. At each humidity levelwe tested viability using embryo rescue (ER), tetrazolium chloride staining and germination in an incubator.We found that seed viabilitywas not reduced when seedswere dried to 4% equilibrium relative humidity (eRH; equating to 2.5% MC). (ii)We assessed viability ofmature and less mature seeds using ER and germination in the soil and tested responses to drying. Findings showed that seeds must be fully mature to germinate and immature seeds had negligible viability. (iii) We dried seeds extracted from ripe/unripe fruit to 35–40% eRH at different rates and tested viability with germination tests in the soil. Seeds from unripe fruit lost viability when dried and especially when dried faster; seeds from ripe fruit only lost viability when fast dried. (iv) Finally, we dried and re-imbibed mature and less mature seeds and measured embryo shrinkage and volume change using X-ray computer tomography. Embryos of less mature seeds shrank significantly when dried to 15% eRH from 0.468 to 0.262 mm3, but embryos of mature seeds did not. Based on our results, mature seeds from ripe fruit are desiccation tolerant to moisture levels required for seed genebanking but embryos from immature seeds are mechanistically less able to withstand desiccation, especially when water potential gradients are high. 2022-01-01 2022-01-19T14:05:57Z 2022-01-19T14:05:57Z Journal Article https://hdl.handle.net/10568/117591 en Open Access application/pdf Oxford University Press Kallow, S.; Garcia Zuluaga, M.; Fanega Sleziak, N.; Nugraha, B.; Mertens, A.; Janssens, S.B.; Gueco, L.; Valle-Descalsota, M.L.; Vu, T.D.; Vu, D.T.; Li, L.T.; Vandelook, F.; Dickie, J.B.; Verboven, P.; Swennen, R.; Panis, B. (2022) Drying banana seeds for ex situ conservation. Conservation Physiology 10(1): coab099. 16 p. ISSN: 2051-1434 |
| spellingShingle | crop wild relatives drought stress genetic resources gene banks especies silvestres afín a las plantas cultivadas estrés de sequia recursos genéticos Kallow, Simon Garcia Zuluaga, Manuela Fanega Sleziak, Natalia Nugraha, Bayu Mertens, Arne Janssens, Steven B. Gueco, Lavernee Valle-Descalsota, Michelle Lyka Vu, Tuong Dang Vu, Dang Toan Li, Loan Thi Vandelook, Filip Dickie, John B. Verboven, Peter Swennen, Rony L. Panis, Bartholomeus Drying banana seeds for ex situ conservation |
| title | Drying banana seeds for ex situ conservation |
| title_full | Drying banana seeds for ex situ conservation |
| title_fullStr | Drying banana seeds for ex situ conservation |
| title_full_unstemmed | Drying banana seeds for ex situ conservation |
| title_short | Drying banana seeds for ex situ conservation |
| title_sort | drying banana seeds for ex situ conservation |
| topic | crop wild relatives drought stress genetic resources gene banks especies silvestres afín a las plantas cultivadas estrés de sequia recursos genéticos |
| url | https://hdl.handle.net/10568/117591 |
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