First report of Fusarium oxysporum f. sp. cubense Tropical Race 4, causing Fusarium wilt in Cavendish bananas in Peru
Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4), the causal agent of Fusarium wilt of banana (FWB), is currently the major threat to the banana industry worldwide (Dita et al. 2018). Restricted to South Asia for more than 20 years, Foc TR4 has spread in the last years to the Middle East,...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Scientific Societies
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/117445 |
| _version_ | 1855534021228888064 |
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| author | Acuña, Rosalyn Rouard, Mathieu Leiva Sandoval, Ana Maria Marques, Claudio Olortegui, Arturo Ureta, Cledy Cabrera-Pintado, Rosa M. Rojas, Juan Carlos López, Diana Cenci, Alberto Cuéllar, Wilmer Jose Dita Rodriguez, Miguel Angel |
| author_browse | Acuña, Rosalyn Cabrera-Pintado, Rosa M. Cenci, Alberto Cuéllar, Wilmer Jose Dita Rodriguez, Miguel Angel Leiva Sandoval, Ana Maria López, Diana Marques, Claudio Olortegui, Arturo Rojas, Juan Carlos Rouard, Mathieu Ureta, Cledy |
| author_facet | Acuña, Rosalyn Rouard, Mathieu Leiva Sandoval, Ana Maria Marques, Claudio Olortegui, Arturo Ureta, Cledy Cabrera-Pintado, Rosa M. Rojas, Juan Carlos López, Diana Cenci, Alberto Cuéllar, Wilmer Jose Dita Rodriguez, Miguel Angel |
| author_sort | Acuña, Rosalyn |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4), the causal agent of Fusarium wilt of banana (FWB), is currently the major threat to the banana industry worldwide (Dita et al. 2018). Restricted to South Asia for more than 20 years, Foc TR4 has spread in the last years to the Middle East, Mozambique, and Colombia (García-Bastidas et al. 2019; https://pestdisplace.org/embed/news/map/disease/11). The incursion of Foc TR4 in Colombia increased awareness and prevention efforts across Latin American and the Caribbean (LAC). However, new Foc TR4 outbreaks in LAC countries were considered a matter of time. In April 2021, banana (Musa spp., Cavendish, AAA) plants (30% of incidence) showing typical symptoms of FWB, such as leave yellowing, wilting and vascular discoloration were observed in one farm (about 1 ha) located in Querecotillo, Peru (4°43’54.84”S 80°33’45.00”W). Mycological analyses of samples (pseudostem strands) collected from 10 symptomatic plants were performed as described by Dita et al. (2010). These analyses revealed a continuous presence of fungal colonies identified as Fusarium oxysporum species complex. Molecular diagnostics targeting two different genome regions (Dita et al 2010; Li et al. 2013) identified nine of these isolates as Foc TR4. These results were further confirmed by qPCR analyses using the commercial Clear Detections TR4 kit. The genome of four single-spore isolates (PerS1, PerS2, PerS3 and PerS4) was sequenced using the Illumina platform (MiSeq Kit, 2x151 bp Paired-End). The strain PerS4 was also sequenced using Oxford Nanopore (FLOW-MIN111; R10.3 chemistry) as described by Lopez-Alvarez et al., (2020). |
| format | Journal Article |
| id | CGSpace117445 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace1174452025-12-08T09:54:28Z First report of Fusarium oxysporum f. sp. cubense Tropical Race 4, causing Fusarium wilt in Cavendish bananas in Peru Acuña, Rosalyn Rouard, Mathieu Leiva Sandoval, Ana Maria Marques, Claudio Olortegui, Arturo Ureta, Cledy Cabrera-Pintado, Rosa M. Rojas, Juan Carlos López, Diana Cenci, Alberto Cuéllar, Wilmer Jose Dita Rodriguez, Miguel Angel fusarium oxysporum panama disease plant disease control enfermedad de panamá control de enfermedades de plantas Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4), the causal agent of Fusarium wilt of banana (FWB), is currently the major threat to the banana industry worldwide (Dita et al. 2018). Restricted to South Asia for more than 20 years, Foc TR4 has spread in the last years to the Middle East, Mozambique, and Colombia (García-Bastidas et al. 2019; https://pestdisplace.org/embed/news/map/disease/11). The incursion of Foc TR4 in Colombia increased awareness and prevention efforts across Latin American and the Caribbean (LAC). However, new Foc TR4 outbreaks in LAC countries were considered a matter of time. In April 2021, banana (Musa spp., Cavendish, AAA) plants (30% of incidence) showing typical symptoms of FWB, such as leave yellowing, wilting and vascular discoloration were observed in one farm (about 1 ha) located in Querecotillo, Peru (4°43’54.84”S 80°33’45.00”W). Mycological analyses of samples (pseudostem strands) collected from 10 symptomatic plants were performed as described by Dita et al. (2010). These analyses revealed a continuous presence of fungal colonies identified as Fusarium oxysporum species complex. Molecular diagnostics targeting two different genome regions (Dita et al 2010; Li et al. 2013) identified nine of these isolates as Foc TR4. These results were further confirmed by qPCR analyses using the commercial Clear Detections TR4 kit. The genome of four single-spore isolates (PerS1, PerS2, PerS3 and PerS4) was sequenced using the Illumina platform (MiSeq Kit, 2x151 bp Paired-End). The strain PerS4 was also sequenced using Oxford Nanopore (FLOW-MIN111; R10.3 chemistry) as described by Lopez-Alvarez et al., (2020). 2022-08-01 2022-01-11T12:38:27Z 2022-01-11T12:38:27Z Journal Article https://hdl.handle.net/10568/117445 en Open Access application/pdf Scientific Societies Acuña, R.; Rouard, M.; Leiva, A.M.; Marques, C.; Olortegui, A.; Ureta, C.; Cabrera-Pintado, R. M.; Rojas, J.C.; López, D.; Cenci, A.; Cuellar, W.J.; Dita, M. (2021) First report of Fusarium oxysporum f. sp. cubense Tropical Race 4, causing Fusarium wilt in Cavendish bananas in Peru. Plant Disease, Online first paper (17 December 2021). ISSN: 0191-2917 |
| spellingShingle | fusarium oxysporum panama disease plant disease control enfermedad de panamá control de enfermedades de plantas Acuña, Rosalyn Rouard, Mathieu Leiva Sandoval, Ana Maria Marques, Claudio Olortegui, Arturo Ureta, Cledy Cabrera-Pintado, Rosa M. Rojas, Juan Carlos López, Diana Cenci, Alberto Cuéllar, Wilmer Jose Dita Rodriguez, Miguel Angel First report of Fusarium oxysporum f. sp. cubense Tropical Race 4, causing Fusarium wilt in Cavendish bananas in Peru |
| title | First report of Fusarium oxysporum f. sp. cubense Tropical Race 4, causing Fusarium wilt in Cavendish bananas in Peru |
| title_full | First report of Fusarium oxysporum f. sp. cubense Tropical Race 4, causing Fusarium wilt in Cavendish bananas in Peru |
| title_fullStr | First report of Fusarium oxysporum f. sp. cubense Tropical Race 4, causing Fusarium wilt in Cavendish bananas in Peru |
| title_full_unstemmed | First report of Fusarium oxysporum f. sp. cubense Tropical Race 4, causing Fusarium wilt in Cavendish bananas in Peru |
| title_short | First report of Fusarium oxysporum f. sp. cubense Tropical Race 4, causing Fusarium wilt in Cavendish bananas in Peru |
| title_sort | first report of fusarium oxysporum f sp cubense tropical race 4 causing fusarium wilt in cavendish bananas in peru |
| topic | fusarium oxysporum panama disease plant disease control enfermedad de panamá control de enfermedades de plantas |
| url | https://hdl.handle.net/10568/117445 |
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