Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)

Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF) form a larg...

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Autores principales: Bengoa Luoni, Sofia A., Cenci, Alberto, Moschen, Sebastian, Nicosia, Salvador, Radonic, Laura M., Sabio Garcia, Julia, Langlade, Nicolas B., Vile, Denis, Vázquez Rovere, Cecilia, Fernandez, Paula
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2021
Materias:
Acceso en línea:https://hdl.handle.net/10568/116803
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author Bengoa Luoni, Sofia A.
Cenci, Alberto
Moschen, Sebastian
Nicosia, Salvador
Radonic, Laura M.
Sabio Garcia, Julia
Langlade, Nicolas B.
Vile, Denis
Vázquez Rovere, Cecilia
Fernandez, Paula
author_browse Bengoa Luoni, Sofia A.
Cenci, Alberto
Fernandez, Paula
Langlade, Nicolas B.
Moschen, Sebastian
Nicosia, Salvador
Radonic, Laura M.
Sabio Garcia, Julia
Vile, Denis
Vázquez Rovere, Cecilia
author_facet Bengoa Luoni, Sofia A.
Cenci, Alberto
Moschen, Sebastian
Nicosia, Salvador
Radonic, Laura M.
Sabio Garcia, Julia
Langlade, Nicolas B.
Vile, Denis
Vázquez Rovere, Cecilia
Fernandez, Paula
author_sort Bengoa Luoni, Sofia A.
collection Repository of Agricultural Research Outputs (CGSpace)
description Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF) form a large plant-specific gene family involved in regulating development, senescence, and responses to biotic and abiotic stresses. The main goal of this work was to identify sunflower NAC TF (HaNAC) and their association with senescence, studying their orthologous to understand possible functional relationships between genes of different species. To clarify the orthologous relationships, we used an in-depth comparative study of four divergent taxa, in dicots and monocots, with completely sequenced genomes (Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa). These orthologous groups provide a curated resource for large scale protein sequence annotation of NAC TF. From the 151 HaNAC genes detected in the latest version of the sunflower genome, 50 genes were associated with senescence traits. These genes showed significant differential expression in two contrasting lines according to an RNAseq assay. An assessment of overexpressing the Arabidopsis line for HaNAC001 (a gene of the same orthologous group of Arabidopsis thaliana ORE1) revealed that this line displayed a significantly higher number of senescent leaves and a pronounced change in development rate. This finding suggests HaNAC001 as an interesting candidate to explore the molecular regulation of senescence in sunflower.
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spelling CGSpace1168032025-12-08T09:54:28Z Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus) Bengoa Luoni, Sofia A. Cenci, Alberto Moschen, Sebastian Nicosia, Salvador Radonic, Laura M. Sabio Garcia, Julia Langlade, Nicolas B. Vile, Denis Vázquez Rovere, Cecilia Fernandez, Paula senescence transcription factors phylogenetic analysis plant breeding avejentamiento factores de transcripción análisis filogenético helianthus annuus Leaf senescence delay impacts positively in grain yield by maintaining the photosynthetic area during the reproductive stage and during grain filling. Therefore a comprehensive understanding of the gene families associated with leaf senescence is essential. NAC transcription factors (TF) form a large plant-specific gene family involved in regulating development, senescence, and responses to biotic and abiotic stresses. The main goal of this work was to identify sunflower NAC TF (HaNAC) and their association with senescence, studying their orthologous to understand possible functional relationships between genes of different species. To clarify the orthologous relationships, we used an in-depth comparative study of four divergent taxa, in dicots and monocots, with completely sequenced genomes (Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa). These orthologous groups provide a curated resource for large scale protein sequence annotation of NAC TF. From the 151 HaNAC genes detected in the latest version of the sunflower genome, 50 genes were associated with senescence traits. These genes showed significant differential expression in two contrasting lines according to an RNAseq assay. An assessment of overexpressing the Arabidopsis line for HaNAC001 (a gene of the same orthologous group of Arabidopsis thaliana ORE1) revealed that this line displayed a significantly higher number of senescent leaves and a pronounced change in development rate. This finding suggests HaNAC001 as an interesting candidate to explore the molecular regulation of senescence in sunflower. 2021-12 2021-12-16T15:58:34Z 2021-12-16T15:58:34Z Journal Article https://hdl.handle.net/10568/116803 en Open Access application/pdf Springer Bengoa Luoni, S.A.; Cenci, A.; Moschen, S.; Nicosia, S.; Radonic, L.M.; Sabio Garcia, J.; Langlade, N.B.; Vile, D.; Vazquez Rovere, C.; Fernandez, P. (2021) Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus). BMC Genomics 22: 893. 19 p. ISSN: 1471-2164
spellingShingle senescence
transcription factors
phylogenetic analysis
plant breeding
avejentamiento
factores de transcripción
análisis filogenético
helianthus annuus
Bengoa Luoni, Sofia A.
Cenci, Alberto
Moschen, Sebastian
Nicosia, Salvador
Radonic, Laura M.
Sabio Garcia, Julia
Langlade, Nicolas B.
Vile, Denis
Vázquez Rovere, Cecilia
Fernandez, Paula
Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
title Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
title_full Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
title_fullStr Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
title_full_unstemmed Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
title_short Genome-wide and comparative phylogenetic analysis of senescence-associated NAC transcription factors in sunflower (Helianthus annuus)
title_sort genome wide and comparative phylogenetic analysis of senescence associated nac transcription factors in sunflower helianthus annuus
topic senescence
transcription factors
phylogenetic analysis
plant breeding
avejentamiento
factores de transcripción
análisis filogenético
helianthus annuus
url https://hdl.handle.net/10568/116803
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