Genome-wide association analysis of bean fly resistance and agromorphological traits in common bean
The bean fly (Ophiomyia spp) is a key insect pest causing significant crop damage and yield loss in common bean (Phaseolus vulgaris L., 2n = 2x = 22). Development and deployment of agronomic superior and bean fly resistant common bean varieties aredependent on genetic variation and the identificatio...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Public Library of Science
2021
|
| Online Access: | https://hdl.handle.net/10568/116784 |
| _version_ | 1855522377220227072 |
|---|---|
| author | Nkhata, Wilson Shimelis, Hussein Melis, Rob Chirwa, Rowland Mzengeza, Tenyson Mathew, Isack Shayanowako, Admire |
| author_browse | Chirwa, Rowland Mathew, Isack Melis, Rob Mzengeza, Tenyson Nkhata, Wilson Shayanowako, Admire Shimelis, Hussein |
| author_facet | Nkhata, Wilson Shimelis, Hussein Melis, Rob Chirwa, Rowland Mzengeza, Tenyson Mathew, Isack Shayanowako, Admire |
| author_sort | Nkhata, Wilson |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The bean fly (Ophiomyia spp) is a key insect pest causing significant crop damage and yield loss in common bean (Phaseolus vulgaris L., 2n = 2x = 22). Development and deployment of agronomic superior and bean fly resistant common bean varieties aredependent on genetic variation and the identification of genes and genomic regions controlling economic traits. This study’s objective was to determine the population structure of a diverse panel of common bean genotypes and deduce associations between bean fly resistance and agronomic traits based on single nucleotide polymorphism (SNP) markers. Ninety-nine common bean genotypes were phenotyped in two seasons at two locations and genotyped with 16 565 SNP markers. The genotypes exhibited significant variation for bean fly damage severity (BDS), plant mortality rate (PMR), and pupa count (PC). Likewise, the genotypes showed significant variation for agro-morphological traits such as days to flowering (DTF), days to maturity (DTM), number of pods per plant (NPP), number of seeds per pod (NSP), and grain yield (GYD). The genotypes were delineated into two populations, which were based on the Andean and Mesoamerican gene pools. The genotypes exhibited a minimum membership coefficient of 0.60 to their respective populations. Eighty-three significant (P<0.01) markers were identified with an average linkage disequilibrium of 0.20 at 12Mb across the 11 chromosomes. Three markers were identified, each having pleiotropic effects on two traits: M100049197 (BDS and NPP), M3379537 (DTF and PC), and M13122571 (NPP and GYD). The identified markers are useful for marker-assisted selection in the breeding program to develop common bean genotypes with resistance to bean fly damage. |
| format | Journal Article |
| id | CGSpace116784 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace1167842025-11-11T17:47:45Z Genome-wide association analysis of bean fly resistance and agromorphological traits in common bean Nkhata, Wilson Shimelis, Hussein Melis, Rob Chirwa, Rowland Mzengeza, Tenyson Mathew, Isack Shayanowako, Admire The bean fly (Ophiomyia spp) is a key insect pest causing significant crop damage and yield loss in common bean (Phaseolus vulgaris L., 2n = 2x = 22). Development and deployment of agronomic superior and bean fly resistant common bean varieties aredependent on genetic variation and the identification of genes and genomic regions controlling economic traits. This study’s objective was to determine the population structure of a diverse panel of common bean genotypes and deduce associations between bean fly resistance and agronomic traits based on single nucleotide polymorphism (SNP) markers. Ninety-nine common bean genotypes were phenotyped in two seasons at two locations and genotyped with 16 565 SNP markers. The genotypes exhibited significant variation for bean fly damage severity (BDS), plant mortality rate (PMR), and pupa count (PC). Likewise, the genotypes showed significant variation for agro-morphological traits such as days to flowering (DTF), days to maturity (DTM), number of pods per plant (NPP), number of seeds per pod (NSP), and grain yield (GYD). The genotypes were delineated into two populations, which were based on the Andean and Mesoamerican gene pools. The genotypes exhibited a minimum membership coefficient of 0.60 to their respective populations. Eighty-three significant (P<0.01) markers were identified with an average linkage disequilibrium of 0.20 at 12Mb across the 11 chromosomes. Three markers were identified, each having pleiotropic effects on two traits: M100049197 (BDS and NPP), M3379537 (DTF and PC), and M13122571 (NPP and GYD). The identified markers are useful for marker-assisted selection in the breeding program to develop common bean genotypes with resistance to bean fly damage. 2021-04-29 2021-12-16T09:40:58Z 2021-12-16T09:40:58Z Journal Article https://hdl.handle.net/10568/116784 en Open Access application/pdf Public Library of Science Nkhata, W.; Shimelis, H.; Melis, R.; Chirwa, R.; Mzengeza, T.; Mathew, I.; Shayanowako, A. (2021) Genome-wide association analysis of bean fly resistance and agromorphological traits in common bean. PLoS ONE 16(4): e0250729 24 p. ISSN: 1932-6203 |
| spellingShingle | Nkhata, Wilson Shimelis, Hussein Melis, Rob Chirwa, Rowland Mzengeza, Tenyson Mathew, Isack Shayanowako, Admire Genome-wide association analysis of bean fly resistance and agromorphological traits in common bean |
| title | Genome-wide association analysis of bean fly resistance and agromorphological traits in common bean |
| title_full | Genome-wide association analysis of bean fly resistance and agromorphological traits in common bean |
| title_fullStr | Genome-wide association analysis of bean fly resistance and agromorphological traits in common bean |
| title_full_unstemmed | Genome-wide association analysis of bean fly resistance and agromorphological traits in common bean |
| title_short | Genome-wide association analysis of bean fly resistance and agromorphological traits in common bean |
| title_sort | genome wide association analysis of bean fly resistance and agromorphological traits in common bean |
| url | https://hdl.handle.net/10568/116784 |
| work_keys_str_mv | AT nkhatawilson genomewideassociationanalysisofbeanflyresistanceandagromorphologicaltraitsincommonbean AT shimelishussein genomewideassociationanalysisofbeanflyresistanceandagromorphologicaltraitsincommonbean AT melisrob genomewideassociationanalysisofbeanflyresistanceandagromorphologicaltraitsincommonbean AT chirwarowland genomewideassociationanalysisofbeanflyresistanceandagromorphologicaltraitsincommonbean AT mzengezatenyson genomewideassociationanalysisofbeanflyresistanceandagromorphologicaltraitsincommonbean AT mathewisack genomewideassociationanalysisofbeanflyresistanceandagromorphologicaltraitsincommonbean AT shayanowakoadmire genomewideassociationanalysisofbeanflyresistanceandagromorphologicaltraitsincommonbean |