Florpyrauxifen-Benzyl Selectivity to Rice

Florpyrauxifen-benzyl (FPB) is a new class of auxinic herbicide developed for selective weed control in rice. This study aimed to evaluate the effect of environmental conditions, P450 inhibitors, rice cultivar response, and gene expression on FPB selectivity in rice. Field experiments established in...

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Main Authors: Velásquez, Juan Camilo, Das Cas Bundt, Angela, Rabaioli Camargo, Edinalvo, Andres, André, Ebeling Viana, Vivian, Hoyos, Verónica
Format: Journal Article
Language:Inglés
Published: MDPI 2021
Subjects:
Online Access:https://hdl.handle.net/10568/116763
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author Velásquez, Juan Camilo
Das Cas Bundt, Angela
Rabaioli Camargo, Edinalvo
Andres, André
Ebeling Viana, Vivian
Hoyos, Verónica
author_browse Andres, André
Das Cas Bundt, Angela
Ebeling Viana, Vivian
Hoyos, Verónica
Rabaioli Camargo, Edinalvo
Velásquez, Juan Camilo
author_facet Velásquez, Juan Camilo
Das Cas Bundt, Angela
Rabaioli Camargo, Edinalvo
Andres, André
Ebeling Viana, Vivian
Hoyos, Verónica
author_sort Velásquez, Juan Camilo
collection Repository of Agricultural Research Outputs (CGSpace)
description Florpyrauxifen-benzyl (FPB) is a new class of auxinic herbicide developed for selective weed control in rice. This study aimed to evaluate the effect of environmental conditions, P450 inhibitors, rice cultivar response, and gene expression on FPB selectivity in rice. Field experiments established in a randomized block design showed that rice plant injury due to two FPB rates (30 and 60 g ai ha−1) was affected by planting time and rice stage at herbicide application. The injury was higher at the earliest planting season and more in younger plants (V2) than larger (V6 and R0). However, no yield reduction was detected. Under greenhouse conditions, two dose-response experiments in a randomized block design showed that spraying malathion (1 kg ha−1) before FPB application did not reduce herbicide selectivity. The addition of two P450 inhibitors (dietholate and piperonyl butoxide, 10 g a.i. seed-kg−1 and 4.2 kg ai ha−1, respectively) decreased the doses to cause 50% of plant injury (ED50) and growth reduction (GR50). However, it seems not to compromise crop selectivity. Pampeira cultivar showed lower ED50 and GR50 than IRGA 424 RI. A growth chamber experiment was conducted in a completely randomized design to evaluate the gene expression of rice plants sprayed with FPB (30 and 60 g ai ha−1). Results showed downregulation of OsWAKL21.2, an esterase probably related to bio-activation of FPB-ester. However, no effect was detected on CYP71A21 monooxygenase and OsGSTL transferase, enzymes probably related to FPB degradation. Further research should focus on understanding FBP bio-activation as the selective mechanism.
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spelling CGSpace1167632025-12-08T09:54:28Z Florpyrauxifen-Benzyl Selectivity to Rice Velásquez, Juan Camilo Das Cas Bundt, Angela Rabaioli Camargo, Edinalvo Andres, André Ebeling Viana, Vivian Hoyos, Verónica herbicide resistance metabolism gene expression resistencia a los herbicidas metabolismo expresión génica Florpyrauxifen-benzyl (FPB) is a new class of auxinic herbicide developed for selective weed control in rice. This study aimed to evaluate the effect of environmental conditions, P450 inhibitors, rice cultivar response, and gene expression on FPB selectivity in rice. Field experiments established in a randomized block design showed that rice plant injury due to two FPB rates (30 and 60 g ai ha−1) was affected by planting time and rice stage at herbicide application. The injury was higher at the earliest planting season and more in younger plants (V2) than larger (V6 and R0). However, no yield reduction was detected. Under greenhouse conditions, two dose-response experiments in a randomized block design showed that spraying malathion (1 kg ha−1) before FPB application did not reduce herbicide selectivity. The addition of two P450 inhibitors (dietholate and piperonyl butoxide, 10 g a.i. seed-kg−1 and 4.2 kg ai ha−1, respectively) decreased the doses to cause 50% of plant injury (ED50) and growth reduction (GR50). However, it seems not to compromise crop selectivity. Pampeira cultivar showed lower ED50 and GR50 than IRGA 424 RI. A growth chamber experiment was conducted in a completely randomized design to evaluate the gene expression of rice plants sprayed with FPB (30 and 60 g ai ha−1). Results showed downregulation of OsWAKL21.2, an esterase probably related to bio-activation of FPB-ester. However, no effect was detected on CYP71A21 monooxygenase and OsGSTL transferase, enzymes probably related to FPB degradation. Further research should focus on understanding FBP bio-activation as the selective mechanism. 2021-12-14 2021-12-15T15:16:38Z 2021-12-15T15:16:38Z Journal Article https://hdl.handle.net/10568/116763 en Open Access application/pdf application/pdf MDPI Velásquez, J.C.; Das Cas Bundt, A.; Rabaioli Camargo, E.; Andres, A.; Ebeling Viana, V.; Hoyos, V.; Plaza, G.; De Avila, L.A. (2021). Florpyrauxifen-Benzyl Selectivity to Rice. Agriculture 11(12): 1270. ISSN: 2077-0472
spellingShingle herbicide resistance
metabolism
gene expression
resistencia a los herbicidas
metabolismo
expresión génica
Velásquez, Juan Camilo
Das Cas Bundt, Angela
Rabaioli Camargo, Edinalvo
Andres, André
Ebeling Viana, Vivian
Hoyos, Verónica
Florpyrauxifen-Benzyl Selectivity to Rice
title Florpyrauxifen-Benzyl Selectivity to Rice
title_full Florpyrauxifen-Benzyl Selectivity to Rice
title_fullStr Florpyrauxifen-Benzyl Selectivity to Rice
title_full_unstemmed Florpyrauxifen-Benzyl Selectivity to Rice
title_short Florpyrauxifen-Benzyl Selectivity to Rice
title_sort florpyrauxifen benzyl selectivity to rice
topic herbicide resistance
metabolism
gene expression
resistencia a los herbicidas
metabolismo
expresión génica
url https://hdl.handle.net/10568/116763
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AT andresandre florpyrauxifenbenzylselectivitytorice
AT ebelingvianavivian florpyrauxifenbenzylselectivitytorice
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