Tsetse blood-meal sources, endosymbionts and trypanosome-associations in the Maasai Mara National Reserve, a wildlife-human-livestock interface
African trypanosomiasis (AT) is a neglected disease of both humans and animals caused by Trypanosoma parasites, which are transmitted by obligate hematophagous tsetse flies (Glossina spp.). Knowledge on tsetse fly vertebrate hosts and the influence of tsetse endosymbionts on trypanosome presence, es...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Public Library of Science
2021
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/116686 |
| _version_ | 1855518765079330816 |
|---|---|
| author | Makhulu, E.E. Villinger, J. Adunga, V.O. Jeneby, M.M. Kimathi, E.M. Mararo, E. Oundo, J.W. Musa, A.A. Wambua, Lillian |
| author_browse | Adunga, V.O. Jeneby, M.M. Kimathi, E.M. Makhulu, E.E. Mararo, E. Musa, A.A. Oundo, J.W. Villinger, J. Wambua, Lillian |
| author_facet | Makhulu, E.E. Villinger, J. Adunga, V.O. Jeneby, M.M. Kimathi, E.M. Mararo, E. Oundo, J.W. Musa, A.A. Wambua, Lillian |
| author_sort | Makhulu, E.E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | African trypanosomiasis (AT) is a neglected disease of both humans and animals caused by Trypanosoma parasites, which are transmitted by obligate hematophagous tsetse flies (Glossina spp.). Knowledge on tsetse fly vertebrate hosts and the influence of tsetse endosymbionts on trypanosome presence, especially in wildlife-human-livestock interfaces, is limited. We identified tsetse species, their blood-meal sources, and correlations between endosymbionts and trypanosome presence in tsetse flies from the trypanosome-endemic Maasai Mara National Reserve (MMNR) in Kenya. Among 1167 tsetse flies (1136 Glossina pallidipes, 31 Glossina swynnertoni) collected from 10 sampling sites, 28 (2.4%) were positive by PCR for trypanosome DNA, most (17/28) being of Trypanosoma vivax species. Blood-meal analyses based on high-resolution melting analysis of vertebrate cytochrome c oxidase 1 and cytochrome b gene PCR products (n = 354) identified humans as the most common vertebrate host (37%), followed by hippopotamus (29.1%), African buffalo (26.3%), elephant (3.39%), and giraffe (0.84%). Flies positive for trypanosome DNA had fed on hippopotamus and buffalo. Tsetse flies were more likely to be positive for trypanosomes if they had the Sodalis glossinidius endosymbiont (P = 0.0002). These findings point to complex interactions of tsetse flies with trypanosomes, endosymbionts, and diverse vertebrate hosts in wildlife ecosystems such as in the MMNR, which should be considered in control programs. These interactions may contribute to the maintenance of tsetse populations and/or persistent circulation of African trypanosomes. Although the African buffalo is a key reservoir of AT, the higher proportion of hippopotamus blood-meals in flies with trypanosome DNA indicates that other wildlife species may be important in AT transmission. No trypanosomes associated with human disease were identified, but the high proportion of human blood-meals identified are indicative of human African trypanosomiasis risk. Our results add to existing data suggesting that Sodalis endosymbionts are associated with increased trypanosome presence in tsetse flies. |
| format | Journal Article |
| id | CGSpace116686 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace1166862025-08-15T13:21:22Z Tsetse blood-meal sources, endosymbionts and trypanosome-associations in the Maasai Mara National Reserve, a wildlife-human-livestock interface Makhulu, E.E. Villinger, J. Adunga, V.O. Jeneby, M.M. Kimathi, E.M. Mararo, E. Oundo, J.W. Musa, A.A. Wambua, Lillian wildlife vertebrates trypanosomosis infectious diseases African trypanosomiasis (AT) is a neglected disease of both humans and animals caused by Trypanosoma parasites, which are transmitted by obligate hematophagous tsetse flies (Glossina spp.). Knowledge on tsetse fly vertebrate hosts and the influence of tsetse endosymbionts on trypanosome presence, especially in wildlife-human-livestock interfaces, is limited. We identified tsetse species, their blood-meal sources, and correlations between endosymbionts and trypanosome presence in tsetse flies from the trypanosome-endemic Maasai Mara National Reserve (MMNR) in Kenya. Among 1167 tsetse flies (1136 Glossina pallidipes, 31 Glossina swynnertoni) collected from 10 sampling sites, 28 (2.4%) were positive by PCR for trypanosome DNA, most (17/28) being of Trypanosoma vivax species. Blood-meal analyses based on high-resolution melting analysis of vertebrate cytochrome c oxidase 1 and cytochrome b gene PCR products (n = 354) identified humans as the most common vertebrate host (37%), followed by hippopotamus (29.1%), African buffalo (26.3%), elephant (3.39%), and giraffe (0.84%). Flies positive for trypanosome DNA had fed on hippopotamus and buffalo. Tsetse flies were more likely to be positive for trypanosomes if they had the Sodalis glossinidius endosymbiont (P = 0.0002). These findings point to complex interactions of tsetse flies with trypanosomes, endosymbionts, and diverse vertebrate hosts in wildlife ecosystems such as in the MMNR, which should be considered in control programs. These interactions may contribute to the maintenance of tsetse populations and/or persistent circulation of African trypanosomes. Although the African buffalo is a key reservoir of AT, the higher proportion of hippopotamus blood-meals in flies with trypanosome DNA indicates that other wildlife species may be important in AT transmission. No trypanosomes associated with human disease were identified, but the high proportion of human blood-meals identified are indicative of human African trypanosomiasis risk. Our results add to existing data suggesting that Sodalis endosymbionts are associated with increased trypanosome presence in tsetse flies. 2021-01-06 2021-12-14T10:11:46Z 2021-12-14T10:11:46Z Journal Article https://hdl.handle.net/10568/116686 en Open Access Public Library of Science Makhulu, E.E., Villinger, J., Adunga, V.O., Jeneby, M.M., Kimathi, E.M., Mararo, E., Oundo, J.W., Musa, A.A. and Wambua, L. 2021. Tsetse blood-meal sources, endosymbionts and trypanosome-associations in the Maasai Mara National Reserve, a wildlife-human-livestock interface. PLOS Neglected Tropical Diseases 15(1): e0008267. |
| spellingShingle | wildlife vertebrates trypanosomosis infectious diseases Makhulu, E.E. Villinger, J. Adunga, V.O. Jeneby, M.M. Kimathi, E.M. Mararo, E. Oundo, J.W. Musa, A.A. Wambua, Lillian Tsetse blood-meal sources, endosymbionts and trypanosome-associations in the Maasai Mara National Reserve, a wildlife-human-livestock interface |
| title | Tsetse blood-meal sources, endosymbionts and trypanosome-associations in the Maasai Mara National Reserve, a wildlife-human-livestock interface |
| title_full | Tsetse blood-meal sources, endosymbionts and trypanosome-associations in the Maasai Mara National Reserve, a wildlife-human-livestock interface |
| title_fullStr | Tsetse blood-meal sources, endosymbionts and trypanosome-associations in the Maasai Mara National Reserve, a wildlife-human-livestock interface |
| title_full_unstemmed | Tsetse blood-meal sources, endosymbionts and trypanosome-associations in the Maasai Mara National Reserve, a wildlife-human-livestock interface |
| title_short | Tsetse blood-meal sources, endosymbionts and trypanosome-associations in the Maasai Mara National Reserve, a wildlife-human-livestock interface |
| title_sort | tsetse blood meal sources endosymbionts and trypanosome associations in the maasai mara national reserve a wildlife human livestock interface |
| topic | wildlife vertebrates trypanosomosis infectious diseases |
| url | https://hdl.handle.net/10568/116686 |
| work_keys_str_mv | AT makhuluee tsetsebloodmealsourcesendosymbiontsandtrypanosomeassociationsinthemaasaimaranationalreserveawildlifehumanlivestockinterface AT villingerj tsetsebloodmealsourcesendosymbiontsandtrypanosomeassociationsinthemaasaimaranationalreserveawildlifehumanlivestockinterface AT adungavo tsetsebloodmealsourcesendosymbiontsandtrypanosomeassociationsinthemaasaimaranationalreserveawildlifehumanlivestockinterface AT jenebymm tsetsebloodmealsourcesendosymbiontsandtrypanosomeassociationsinthemaasaimaranationalreserveawildlifehumanlivestockinterface AT kimathiem tsetsebloodmealsourcesendosymbiontsandtrypanosomeassociationsinthemaasaimaranationalreserveawildlifehumanlivestockinterface AT mararoe tsetsebloodmealsourcesendosymbiontsandtrypanosomeassociationsinthemaasaimaranationalreserveawildlifehumanlivestockinterface AT oundojw tsetsebloodmealsourcesendosymbiontsandtrypanosomeassociationsinthemaasaimaranationalreserveawildlifehumanlivestockinterface AT musaaa tsetsebloodmealsourcesendosymbiontsandtrypanosomeassociationsinthemaasaimaranationalreserveawildlifehumanlivestockinterface AT wambualillian tsetsebloodmealsourcesendosymbiontsandtrypanosomeassociationsinthemaasaimaranationalreserveawildlifehumanlivestockinterface |