Somaclonal variation in clonal crops: containing the bad, exploring the good
Somaclonal variation describes random cellular changes in plants regenerated through tissue culture. It occurs in certain crops that undergo micropropagation and has been recorded in different explant sources, from leaves and shoots to meristems and embryos. In banana (Musa spp.), a clonal crop cons...
| Main Authors: | , , , , , , , |
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| Format: | Book Chapter |
| Language: | Inglés |
| Published: |
CAB International
2021
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/116597 |
| _version_ | 1855528118524051456 |
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| author | Roux, Nicolas Stephan M. Chase, Rachel Houwe, Ines van den Chao, Chih-Ping Perrier, Xavier Jacquemoud-Collet, Jean-Pierre Sardos, Julie Rouard, Mathieu |
| author_browse | Chao, Chih-Ping Chase, Rachel Houwe, Ines van den Jacquemoud-Collet, Jean-Pierre Perrier, Xavier Rouard, Mathieu Roux, Nicolas Stephan M. Sardos, Julie |
| author_facet | Roux, Nicolas Stephan M. Chase, Rachel Houwe, Ines van den Chao, Chih-Ping Perrier, Xavier Jacquemoud-Collet, Jean-Pierre Sardos, Julie Rouard, Mathieu |
| author_sort | Roux, Nicolas Stephan M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Somaclonal variation describes random cellular changes in plants regenerated through tissue culture. It occurs in certain crops that undergo micropropagation and has been recorded in different explant sources, from leaves and shoots to meristems and embryos. In banana (Musa spp.), a clonal crop conserved in vitro, somaclonal variation has been observed after prolonged periods in tissue culture, resulting from an increase in subcultures performed on a given clone. According to scientific literature, variants, or off-types, often show characteristics such as abnormal growth and flower or fruit defects in frequencies ranging from 1% to 32%. This variation poses a problem for gene bank managers, whose mandate is to maintain the genetic integrity of their collections for research and breeding. In the case of the Bioversity International Musa Germplasm Transit Centre (ITC), stress
during the in vitro process is minimized by various techniques and plants are regenerated after 10 years, making it a long and costly process. Identifying somaclonal variation at an early stage would be an ideal solution; however, this requires suitable molecular markers. Recent studies revealed that techniques such as direct DNA sequencing and single nucleotide olymorphisms (SNPs) are able to detect the underlying factors of somaclonal variation and are becoming more accessible. On the other hand, somaclonal variation can be beneficial as it allows the natural development of new varieties and supplies genetic stocks used for future genetic studies. Harnessing the diversity of somaclones is easier, faster and cheaper compared with other methods of crop improvement, al though it is also less predictable. So far, variants of crops such as apple, strawberry, potato and banana have been successfully adopted into global markets. In this chapter, we will discuss how to minimize the adverse effects of somaclonal variation while maximizing its benefits for greater crop diversity, with a particular focus on banana. |
| format | Book Chapter |
| id | CGSpace116597 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | CAB International |
| publisherStr | CAB International |
| record_format | dspace |
| spelling | CGSpace1165972025-11-05T11:55:30Z Somaclonal variation in clonal crops: containing the bad, exploring the good Roux, Nicolas Stephan M. Chase, Rachel Houwe, Ines van den Chao, Chih-Ping Perrier, Xavier Jacquemoud-Collet, Jean-Pierre Sardos, Julie Rouard, Mathieu somaclonal variation crop improvement mutation breeding variación somaclonal mejora de cultivos mejoramiento por mutación Somaclonal variation describes random cellular changes in plants regenerated through tissue culture. It occurs in certain crops that undergo micropropagation and has been recorded in different explant sources, from leaves and shoots to meristems and embryos. In banana (Musa spp.), a clonal crop conserved in vitro, somaclonal variation has been observed after prolonged periods in tissue culture, resulting from an increase in subcultures performed on a given clone. According to scientific literature, variants, or off-types, often show characteristics such as abnormal growth and flower or fruit defects in frequencies ranging from 1% to 32%. This variation poses a problem for gene bank managers, whose mandate is to maintain the genetic integrity of their collections for research and breeding. In the case of the Bioversity International Musa Germplasm Transit Centre (ITC), stress during the in vitro process is minimized by various techniques and plants are regenerated after 10 years, making it a long and costly process. Identifying somaclonal variation at an early stage would be an ideal solution; however, this requires suitable molecular markers. Recent studies revealed that techniques such as direct DNA sequencing and single nucleotide olymorphisms (SNPs) are able to detect the underlying factors of somaclonal variation and are becoming more accessible. On the other hand, somaclonal variation can be beneficial as it allows the natural development of new varieties and supplies genetic stocks used for future genetic studies. Harnessing the diversity of somaclones is easier, faster and cheaper compared with other methods of crop improvement, al though it is also less predictable. So far, variants of crops such as apple, strawberry, potato and banana have been successfully adopted into global markets. In this chapter, we will discuss how to minimize the adverse effects of somaclonal variation while maximizing its benefits for greater crop diversity, with a particular focus on banana. 2021-11 2021-12-08T11:50:19Z 2021-12-08T11:50:19Z Book Chapter https://hdl.handle.net/10568/116597 en Open Access application/pdf CAB International Roux, N.; Chase, R.; Van den Houwe, I.; Chao, C.-P.; Perrier, X.; Jacquemoud-Collet, J.-P.; Sardos, J.; Rouard, M. (2021) Somaclonal variation in clonal crops: containing the bad, exploring the good. In: (Sivasankar, S. (eds.) et al.) Mutation breeding, genetic diversity and crop adaptation to climate change. Oxfordshire (UK): CABI. p. 355-365. ISBN: 9781789249101 |
| spellingShingle | somaclonal variation crop improvement mutation breeding variación somaclonal mejora de cultivos mejoramiento por mutación Roux, Nicolas Stephan M. Chase, Rachel Houwe, Ines van den Chao, Chih-Ping Perrier, Xavier Jacquemoud-Collet, Jean-Pierre Sardos, Julie Rouard, Mathieu Somaclonal variation in clonal crops: containing the bad, exploring the good |
| title | Somaclonal variation in clonal crops: containing the bad, exploring the good |
| title_full | Somaclonal variation in clonal crops: containing the bad, exploring the good |
| title_fullStr | Somaclonal variation in clonal crops: containing the bad, exploring the good |
| title_full_unstemmed | Somaclonal variation in clonal crops: containing the bad, exploring the good |
| title_short | Somaclonal variation in clonal crops: containing the bad, exploring the good |
| title_sort | somaclonal variation in clonal crops containing the bad exploring the good |
| topic | somaclonal variation crop improvement mutation breeding variación somaclonal mejora de cultivos mejoramiento por mutación |
| url | https://hdl.handle.net/10568/116597 |
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