ILRI In-House reconstituted SARS-CoV-2 RT-qPCR testing kit as an alternative to the commercial kits
Due to worldwide shortage and the increasing cost of COVID-19 testing kits, International Livestock Research Institute (ILRI) scientists have developed in-house reconstituted testing reagents based on the known primer sequences and their targets on the SARS-COV-2 genome. The ILRI In-House reconstitu...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | News Item |
| Language: | Inglés |
| Published: |
2021
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/116082 |
| _version_ | 1855536706860613632 |
|---|---|
| author | Mwangi, Reuben Osiany, Shebbar Kiritu, Edward Dobi, Paul Ouso, Daniel Kibet, Gilbert Njeru, Regina Oyola, Samuel O. |
| author_browse | Dobi, Paul Kibet, Gilbert Kiritu, Edward Mwangi, Reuben Njeru, Regina Osiany, Shebbar Ouso, Daniel Oyola, Samuel O. |
| author_facet | Mwangi, Reuben Osiany, Shebbar Kiritu, Edward Dobi, Paul Ouso, Daniel Kibet, Gilbert Njeru, Regina Oyola, Samuel O. |
| author_sort | Mwangi, Reuben |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Due to worldwide shortage and the increasing cost of COVID-19 testing kits, International Livestock Research Institute (ILRI) scientists have developed in-house reconstituted testing reagents based on the known primer sequences and their targets on the SARS-COV-2 genome. The ILRI In-House reconstituted kit reduces the cost of COVID-19 RT-qPCR testing by at least 50% compared to the cheapest commercial kits available.
Our ILRI In-House reconstituted kit is a three-target multiplex formation consisting of IDT synthesized primer and probes targeting two SARS-COV-2 genes (ORF1ab and N) and an internal control targeting the human RNAse P gene. The reconstituted kit uses an RT-qPCR Mastermix with low Rox for RT-PCR amplification.
We have thoroughly conducted validation tests on the ILRI In-House reconstituted kit by comparing results of the same/matching samples generated by approved commercial kits for SARS-COV-2 testing. The commercial kits used for validation experiments include DA0990-Detection Kit (Da An Gene) and TaqPath™ COVID-19 CE-IVD RT-PCR Kit (Applied Biosystems) to which we have obtained 98.96% concordance. Detailed documentation of our validation results is available. |
| format | News Item |
| id | CGSpace116082 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| record_format | dspace |
| spelling | CGSpace1160822025-11-04T20:16:35Z ILRI In-House reconstituted SARS-CoV-2 RT-qPCR testing kit as an alternative to the commercial kits Mwangi, Reuben Osiany, Shebbar Kiritu, Edward Dobi, Paul Ouso, Daniel Kibet, Gilbert Njeru, Regina Oyola, Samuel O. covid-19 diagnosis health coronavirus disease Due to worldwide shortage and the increasing cost of COVID-19 testing kits, International Livestock Research Institute (ILRI) scientists have developed in-house reconstituted testing reagents based on the known primer sequences and their targets on the SARS-COV-2 genome. The ILRI In-House reconstituted kit reduces the cost of COVID-19 RT-qPCR testing by at least 50% compared to the cheapest commercial kits available. Our ILRI In-House reconstituted kit is a three-target multiplex formation consisting of IDT synthesized primer and probes targeting two SARS-COV-2 genes (ORF1ab and N) and an internal control targeting the human RNAse P gene. The reconstituted kit uses an RT-qPCR Mastermix with low Rox for RT-PCR amplification. We have thoroughly conducted validation tests on the ILRI In-House reconstituted kit by comparing results of the same/matching samples generated by approved commercial kits for SARS-COV-2 testing. The commercial kits used for validation experiments include DA0990-Detection Kit (Da An Gene) and TaqPath™ COVID-19 CE-IVD RT-PCR Kit (Applied Biosystems) to which we have obtained 98.96% concordance. Detailed documentation of our validation results is available. 2021-11-12 2021-11-15T12:30:31Z 2021-11-15T12:30:31Z News Item https://hdl.handle.net/10568/116082 en Open Access application/pdf Mwangi, R., Osiany, S., Kiritu, E., Dobi, P., Ouso, D., Kibet, G., Njeru, R. and Oyola, S.O. 2021. ILRI In-House reconstituted SARS-CoV-2 RT-qPCR testing kit as an alternative to the commercial kits. Abstract. Nairobi, Kenya: ILRI. |
| spellingShingle | covid-19 diagnosis health coronavirus disease Mwangi, Reuben Osiany, Shebbar Kiritu, Edward Dobi, Paul Ouso, Daniel Kibet, Gilbert Njeru, Regina Oyola, Samuel O. ILRI In-House reconstituted SARS-CoV-2 RT-qPCR testing kit as an alternative to the commercial kits |
| title | ILRI In-House reconstituted SARS-CoV-2 RT-qPCR testing kit as an alternative to the commercial kits |
| title_full | ILRI In-House reconstituted SARS-CoV-2 RT-qPCR testing kit as an alternative to the commercial kits |
| title_fullStr | ILRI In-House reconstituted SARS-CoV-2 RT-qPCR testing kit as an alternative to the commercial kits |
| title_full_unstemmed | ILRI In-House reconstituted SARS-CoV-2 RT-qPCR testing kit as an alternative to the commercial kits |
| title_short | ILRI In-House reconstituted SARS-CoV-2 RT-qPCR testing kit as an alternative to the commercial kits |
| title_sort | ilri in house reconstituted sars cov 2 rt qpcr testing kit as an alternative to the commercial kits |
| topic | covid-19 diagnosis health coronavirus disease |
| url | https://hdl.handle.net/10568/116082 |
| work_keys_str_mv | AT mwangireuben ilriinhousereconstitutedsarscov2rtqpcrtestingkitasanalternativetothecommercialkits AT osianyshebbar ilriinhousereconstitutedsarscov2rtqpcrtestingkitasanalternativetothecommercialkits AT kirituedward ilriinhousereconstitutedsarscov2rtqpcrtestingkitasanalternativetothecommercialkits AT dobipaul ilriinhousereconstitutedsarscov2rtqpcrtestingkitasanalternativetothecommercialkits AT ousodaniel ilriinhousereconstitutedsarscov2rtqpcrtestingkitasanalternativetothecommercialkits AT kibetgilbert ilriinhousereconstitutedsarscov2rtqpcrtestingkitasanalternativetothecommercialkits AT njeruregina ilriinhousereconstitutedsarscov2rtqpcrtestingkitasanalternativetothecommercialkits AT oyolasamuelo ilriinhousereconstitutedsarscov2rtqpcrtestingkitasanalternativetothecommercialkits |