Flow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass Urochloa
Urochloa (including Brachiaria, Megathyrus and some Panicum) tropical grasses are native to Africa and are now, after selection and breeding, planted worldwide, particularly in South America, as important forages with huge potential for further sustainable improvement and conservation of grasslands....
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2021
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/115921 |
| _version_ | 1855523169716142080 |
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| author | Tomaszewska, Paulina Pellny, Till K. Hernández, Luis Miguel Mitchell, Rowan A. C. Castiblanco, Valheria Vega, Jose J. de Schwarzacher, Trude Heslop-Harrison, J.S. |
| author_browse | Castiblanco, Valheria Hernández, Luis Miguel Heslop-Harrison, J.S. Mitchell, Rowan A. C. Pellny, Till K. Schwarzacher, Trude Tomaszewska, Paulina Vega, Jose J. de |
| author_facet | Tomaszewska, Paulina Pellny, Till K. Hernández, Luis Miguel Mitchell, Rowan A. C. Castiblanco, Valheria Vega, Jose J. de Schwarzacher, Trude Heslop-Harrison, J.S. |
| author_sort | Tomaszewska, Paulina |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Urochloa (including Brachiaria, Megathyrus and some Panicum) tropical grasses are native to Africa and are now, after selection and breeding, planted worldwide, particularly in South America, as important forages with huge potential for further sustainable improvement and conservation of grasslands. We aimed to develop an optimized approach to determine ploidy of germplasm collection of this tropical forage grass group using dried leaf material, including approaches to collect, dry and preserve plant samples for flow cytometry analysis. Our methods enable robust identification of ploidy levels (coefficient of variation of G0/G1 peaks, CV, typically <5%). Ploidy of some 348 forage grass accessions (ploidy range from 2x to 9x), from international genetic resource collections, showing variation in basic chromosome numbers and reproduction modes (apomixis and sexual), were determined using our defined standard protocol. Two major Urochloa agamic complexes are used in the current breeding programs at CIAT and EMBRAPA: the ’brizantha’ and ’humidicola’ agamic complexes are variable, with multiple ploidy levels. Some U. brizantha accessions have odd level of ploidy (5x), and the relative differences in fluorescence values of the peak positions between adjacent cytotypes is reduced, thus more precise examination of this species is required. Ploidy measurement of U. humidicola revealed aneuploidy. |
| format | Journal Article |
| id | CGSpace115921 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1159212025-11-11T18:47:06Z Flow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass Urochloa Tomaszewska, Paulina Pellny, Till K. Hernández, Luis Miguel Mitchell, Rowan A. C. Castiblanco, Valheria Vega, Jose J. de Schwarzacher, Trude Heslop-Harrison, J.S. brachiaria panicum feed grasses ploidy flow cytometry apomixis dry matter leguminosas forrajeras ploidía citometría de flujo (células) extracto seco Urochloa (including Brachiaria, Megathyrus and some Panicum) tropical grasses are native to Africa and are now, after selection and breeding, planted worldwide, particularly in South America, as important forages with huge potential for further sustainable improvement and conservation of grasslands. We aimed to develop an optimized approach to determine ploidy of germplasm collection of this tropical forage grass group using dried leaf material, including approaches to collect, dry and preserve plant samples for flow cytometry analysis. Our methods enable robust identification of ploidy levels (coefficient of variation of G0/G1 peaks, CV, typically <5%). Ploidy of some 348 forage grass accessions (ploidy range from 2x to 9x), from international genetic resource collections, showing variation in basic chromosome numbers and reproduction modes (apomixis and sexual), were determined using our defined standard protocol. Two major Urochloa agamic complexes are used in the current breeding programs at CIAT and EMBRAPA: the ’brizantha’ and ’humidicola’ agamic complexes are variable, with multiple ploidy levels. Some U. brizantha accessions have odd level of ploidy (5x), and the relative differences in fluorescence values of the peak positions between adjacent cytotypes is reduced, thus more precise examination of this species is required. Ploidy measurement of U. humidicola revealed aneuploidy. 2021-06-23 2021-11-09T15:21:53Z 2021-11-09T15:21:53Z Journal Article https://hdl.handle.net/10568/115921 en Open Access application/pdf MDPI Tomaszewska, P.; Pellny, T.K.; Hernández, L.M.; Mitchell, R.A.C.; Castiblanco, V.; de Vega, J.J.; Schwarzacher, T.; Heslop-Harrison, P. (2021) Flow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass Urochloa s. l. Genes. 12(7):957. ISSN: 2073-4425 |
| spellingShingle | brachiaria panicum feed grasses ploidy flow cytometry apomixis dry matter leguminosas forrajeras ploidía citometría de flujo (células) extracto seco Tomaszewska, Paulina Pellny, Till K. Hernández, Luis Miguel Mitchell, Rowan A. C. Castiblanco, Valheria Vega, Jose J. de Schwarzacher, Trude Heslop-Harrison, J.S. Flow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass Urochloa |
| title | Flow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass Urochloa |
| title_full | Flow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass Urochloa |
| title_fullStr | Flow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass Urochloa |
| title_full_unstemmed | Flow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass Urochloa |
| title_short | Flow cytometry-based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass Urochloa |
| title_sort | flow cytometry based determination of ploidy from dried leaf specimens in genomically complex collections of the tropical forage grass urochloa |
| topic | brachiaria panicum feed grasses ploidy flow cytometry apomixis dry matter leguminosas forrajeras ploidía citometría de flujo (células) extracto seco |
| url | https://hdl.handle.net/10568/115921 |
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