Report on sampling leave tissue, DNA extraction and prepare for shipment for whole genome sequencing
In the modernization process of Urochloa breeding program of the Bioversity-CIAT alliance, novelty techniques have been implemented to accelerate genetic gain. Some of these techniques include i) field and greenhouse high-throughput phenotyping technologies to increase traits evaluated and their acc...
| Main Authors: | , , |
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| Format: | Informe técnico |
| Language: | Inglés |
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Bioversity International and the International Center for Tropical Agriculture
2021
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/115909 |
| _version_ | 1855525201024909312 |
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| author | Hernández, Luis Miguel Castiblanco, Valheria Florian, David |
| author_browse | Castiblanco, Valheria Florian, David Hernández, Luis Miguel |
| author_facet | Hernández, Luis Miguel Castiblanco, Valheria Florian, David |
| author_sort | Hernández, Luis Miguel |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In the modernization process of Urochloa breeding program of the Bioversity-CIAT alliance, novelty techniques have been implemented to accelerate genetic gain. Some of these techniques include i) field and greenhouse high-throughput phenotyping technologies to increase traits evaluated and their accuracy, ii) molecular markers to use in mass-assisted selection, and iii) flowering induction through prolonged photoperiods to accelerate the developmental rate of plants and flowering index. A novelty technique developed in other crops, called genomic selection, uses both molecular and phenotypic data in an initial population to estimated breeding values of each genotype in a new population that has been genotyped but not phenotyped. In 2020, the apomictic population Br19 was phenotyped. To complement phenotypic information with genotypic information and estimate breeding values of each Br19 hybrids and to take the first steps to implement genomic selection, during 2020 all Br19 genotypes were sampled and sent for whole genome sequencing. This report shows the protocol followed for collection, lyophilization and maceration of Br19 genotypes. |
| format | Informe técnico |
| id | CGSpace115909 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Bioversity International and the International Center for Tropical Agriculture |
| publisherStr | Bioversity International and the International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace1159092025-12-08T10:29:22Z Report on sampling leave tissue, DNA extraction and prepare for shipment for whole genome sequencing Hernández, Luis Miguel Castiblanco, Valheria Florian, David brachiaria plant breeding genomics fitomejoramiento genómica In the modernization process of Urochloa breeding program of the Bioversity-CIAT alliance, novelty techniques have been implemented to accelerate genetic gain. Some of these techniques include i) field and greenhouse high-throughput phenotyping technologies to increase traits evaluated and their accuracy, ii) molecular markers to use in mass-assisted selection, and iii) flowering induction through prolonged photoperiods to accelerate the developmental rate of plants and flowering index. A novelty technique developed in other crops, called genomic selection, uses both molecular and phenotypic data in an initial population to estimated breeding values of each genotype in a new population that has been genotyped but not phenotyped. In 2020, the apomictic population Br19 was phenotyped. To complement phenotypic information with genotypic information and estimate breeding values of each Br19 hybrids and to take the first steps to implement genomic selection, during 2020 all Br19 genotypes were sampled and sent for whole genome sequencing. This report shows the protocol followed for collection, lyophilization and maceration of Br19 genotypes. 2021-09 2021-11-09T10:33:14Z 2021-11-09T10:33:14Z Report https://hdl.handle.net/10568/115909 en Open Access application/pdf Bioversity International and the International Center for Tropical Agriculture Hernandez, L.; Castiblanco, V.; Florian, D. (2021) Report on sampling leave tissue, DNA extraction and prepare for shipment for whole genome sequencing. Cali (Colombia): Alliance of Bioversity and CIAT. 7 p. |
| spellingShingle | brachiaria plant breeding genomics fitomejoramiento genómica Hernández, Luis Miguel Castiblanco, Valheria Florian, David Report on sampling leave tissue, DNA extraction and prepare for shipment for whole genome sequencing |
| title | Report on sampling leave tissue, DNA extraction and prepare for shipment for whole genome sequencing |
| title_full | Report on sampling leave tissue, DNA extraction and prepare for shipment for whole genome sequencing |
| title_fullStr | Report on sampling leave tissue, DNA extraction and prepare for shipment for whole genome sequencing |
| title_full_unstemmed | Report on sampling leave tissue, DNA extraction and prepare for shipment for whole genome sequencing |
| title_short | Report on sampling leave tissue, DNA extraction and prepare for shipment for whole genome sequencing |
| title_sort | report on sampling leave tissue dna extraction and prepare for shipment for whole genome sequencing |
| topic | brachiaria plant breeding genomics fitomejoramiento genómica |
| url | https://hdl.handle.net/10568/115909 |
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