Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance
Spittlebugs (Hemiptera: Cercopidae) are the major production limitation for Brachiaria grasses (Urochloa spp.) in the tropical America. Incorporate host-plant resistance to new hybrids is the most cost-effective and sustainable solution to overcome the great losses caused by this key pest. Identifyi...
| Autores principales: | , , , , |
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| Formato: | Informe técnico |
| Lenguaje: | Inglés |
| Publicado: |
Bioversity International and the International Center for Tropical Agriculture
2021
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/115879 |
| _version_ | 1855539103121014784 |
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| author | Espitia, Paula A. Hernández, Luis Miguel Villegas, Daniel Mauricio Arango, Jacobo Castiblanco, Valheria |
| author_browse | Arango, Jacobo Castiblanco, Valheria Espitia, Paula A. Hernández, Luis Miguel Villegas, Daniel Mauricio |
| author_facet | Espitia, Paula A. Hernández, Luis Miguel Villegas, Daniel Mauricio Arango, Jacobo Castiblanco, Valheria |
| author_sort | Espitia, Paula A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Spittlebugs (Hemiptera: Cercopidae) are the major production limitation for Brachiaria grasses (Urochloa spp.) in the tropical America. Incorporate host-plant resistance to new hybrids is the most cost-effective and sustainable solution to overcome the great losses caused by this key pest. Identifying regions in Urochloa genome associated with resistance to Aeneolamia varia nymphal attack, would allow to develop genotyping tools (e.g., molecular markers) that contribute to the hybrid’s selection in Urochloa interspecific breeding pipeline. Leaf tissue was extracted from 315 genotypes from the three biparental populations constructed for QTL analysis. These samples are kept at -20ºC, to be lyophilized and shipped for genotyping through RAD sequencing. |
| format | Informe técnico |
| id | CGSpace115879 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Bioversity International and the International Center for Tropical Agriculture |
| publisherStr | Bioversity International and the International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace1158792025-12-08T10:29:22Z Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance Espitia, Paula A. Hernández, Luis Miguel Villegas, Daniel Mauricio Arango, Jacobo Castiblanco, Valheria forage sampling genotypes dna pest resistance forrajes muestreo genotipos resistencia a las plagas adn cercopidae Spittlebugs (Hemiptera: Cercopidae) are the major production limitation for Brachiaria grasses (Urochloa spp.) in the tropical America. Incorporate host-plant resistance to new hybrids is the most cost-effective and sustainable solution to overcome the great losses caused by this key pest. Identifying regions in Urochloa genome associated with resistance to Aeneolamia varia nymphal attack, would allow to develop genotyping tools (e.g., molecular markers) that contribute to the hybrid’s selection in Urochloa interspecific breeding pipeline. Leaf tissue was extracted from 315 genotypes from the three biparental populations constructed for QTL analysis. These samples are kept at -20ºC, to be lyophilized and shipped for genotyping through RAD sequencing. 2021-09 2021-11-08T11:26:12Z 2021-11-08T11:26:12Z Report https://hdl.handle.net/10568/115879 en Open Access application/pdf Bioversity International and the International Center for Tropical Agriculture Espitia, P.; Hernández L.M.; Villegas, D.; Arango, J.; Castiblanco, V. (2021) Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance. Cali (Colombia): Alliance of Bioversity and CIAT. 5 p. |
| spellingShingle | forage sampling genotypes dna pest resistance forrajes muestreo genotipos resistencia a las plagas adn cercopidae Espitia, Paula A. Hernández, Luis Miguel Villegas, Daniel Mauricio Arango, Jacobo Castiblanco, Valheria Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance |
| title | Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance |
| title_full | Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance |
| title_fullStr | Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance |
| title_full_unstemmed | Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance |
| title_short | Report on sampling leaf tissue, DNA extraction and prepare for shipment for genotyping of two biparental populations to identify QTLs associated with Spittlebug resistance |
| title_sort | report on sampling leaf tissue dna extraction and prepare for shipment for genotyping of two biparental populations to identify qtls associated with spittlebug resistance |
| topic | forage sampling genotypes dna pest resistance forrajes muestreo genotipos resistencia a las plagas adn cercopidae |
| url | https://hdl.handle.net/10568/115879 |
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