Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis
Melilotus species are used as green manure and rotation crops worldwide and contain abundant pharmacologically active coumarins. However, there is a paucity of information on its genome and coumarin production and function. Here, we reported a chromosome‐scale assembly of Melilotus albus genome with...
| Autores principales: | , , , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/115873 |
| _version_ | 1855534618540769280 |
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| author | Fan Wu Zhen Duan Pan Xu Qi Yan Minghui Meng Cao, M. Jones, Christopher S. Xifang Zong Pei Zhou Yimeng Wang Kai Luo Shengsheng Wang Zhuanzhuan Yan Penglei Wang Hongyan Di Zifeng Ouyang Yanrong Wang Jiyu Zhang |
| author_browse | Cao, M. Fan Wu Hongyan Di Jiyu Zhang Jones, Christopher S. Kai Luo Minghui Meng Pan Xu Pei Zhou Penglei Wang Qi Yan Shengsheng Wang Xifang Zong Yanrong Wang Yimeng Wang Zhen Duan Zhuanzhuan Yan Zifeng Ouyang |
| author_facet | Fan Wu Zhen Duan Pan Xu Qi Yan Minghui Meng Cao, M. Jones, Christopher S. Xifang Zong Pei Zhou Yimeng Wang Kai Luo Shengsheng Wang Zhuanzhuan Yan Penglei Wang Hongyan Di Zifeng Ouyang Yanrong Wang Jiyu Zhang |
| author_sort | Fan Wu |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Melilotus species are used as green manure and rotation crops worldwide and contain abundant pharmacologically active coumarins. However, there is a paucity of information on its genome and coumarin production and function. Here, we reported a chromosome‐scale assembly of Melilotus albus genome with 1.04 Gb in eight chromosomes, containing 71.42% repetitive elements. Long terminal repeat retrotransposon bursts coincided with declining of population sizes during the Quaternary glaciation. Resequencing of 94 accessions enabled insights into genetic diversity, population structure, and introgression. Melilotus officinalis had relatively larger genetic diversity than that of M. albus. The introgression existed between M. officinalis group and M. albus group, and gene flows was from M. albus to M. officinalis. Selection sweep analysis identified candidate genes associated with flower colour and coumarin biosynthesis. Combining genomics, BSA, transcriptomics, metabolomics, and biochemistry, we identified a β‐glucosidase (BGLU) gene cluster contributing to coumarin biosynthesis. MaBGLU1 function was verified by overexpression in M. albus, heterologous expression in Escherichia coli, and substrate feeding, revealing its role in scopoletin (coumarin derivative) production and showing that nonsynonymous variation drives BGLU enzyme activity divergence in Melilotus. Our work will accelerate the understanding of biologically active coumarins and their biosynthetic pathways, and contribute to genomics‐enabled Melilotus breeding. |
| format | Journal Article |
| id | CGSpace115873 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1158732024-08-27T10:36:44Z Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis Fan Wu Zhen Duan Pan Xu Qi Yan Minghui Meng Cao, M. Jones, Christopher S. Xifang Zong Pei Zhou Yimeng Wang Kai Luo Shengsheng Wang Zhuanzhuan Yan Penglei Wang Hongyan Di Zifeng Ouyang Yanrong Wang Jiyu Zhang biotechnology melilotus alba plant biotechnology crops Melilotus species are used as green manure and rotation crops worldwide and contain abundant pharmacologically active coumarins. However, there is a paucity of information on its genome and coumarin production and function. Here, we reported a chromosome‐scale assembly of Melilotus albus genome with 1.04 Gb in eight chromosomes, containing 71.42% repetitive elements. Long terminal repeat retrotransposon bursts coincided with declining of population sizes during the Quaternary glaciation. Resequencing of 94 accessions enabled insights into genetic diversity, population structure, and introgression. Melilotus officinalis had relatively larger genetic diversity than that of M. albus. The introgression existed between M. officinalis group and M. albus group, and gene flows was from M. albus to M. officinalis. Selection sweep analysis identified candidate genes associated with flower colour and coumarin biosynthesis. Combining genomics, BSA, transcriptomics, metabolomics, and biochemistry, we identified a β‐glucosidase (BGLU) gene cluster contributing to coumarin biosynthesis. MaBGLU1 function was verified by overexpression in M. albus, heterologous expression in Escherichia coli, and substrate feeding, revealing its role in scopoletin (coumarin derivative) production and showing that nonsynonymous variation drives BGLU enzyme activity divergence in Melilotus. Our work will accelerate the understanding of biologically active coumarins and their biosynthetic pathways, and contribute to genomics‐enabled Melilotus breeding. 2022-03 2021-11-08T10:43:12Z 2021-11-08T10:43:12Z Journal Article https://hdl.handle.net/10568/115873 en Open Access Wiley Fan Wu, Zhen Duan, Pan Xu, Qi Yan, Minghui Meng, Cao, M., Jones, C.S., Xifang Zong, Pei Zhou, Yimeng Wang, Kai Luo, Shengsheng Wang, Zhuanzhuan Yan, Penglei Wang, Hongyan Di, Zifeng Ouyang, Yanrong Wang and Jiyu Zhang. 2021. Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis. Plant Biotechnology Journal |
| spellingShingle | biotechnology melilotus alba plant biotechnology crops Fan Wu Zhen Duan Pan Xu Qi Yan Minghui Meng Cao, M. Jones, Christopher S. Xifang Zong Pei Zhou Yimeng Wang Kai Luo Shengsheng Wang Zhuanzhuan Yan Penglei Wang Hongyan Di Zifeng Ouyang Yanrong Wang Jiyu Zhang Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis |
| title | Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis |
| title_full | Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis |
| title_fullStr | Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis |
| title_full_unstemmed | Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis |
| title_short | Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis |
| title_sort | genome and systems biology of melilotus albus provides insights into coumarins biosynthesis |
| topic | biotechnology melilotus alba plant biotechnology crops |
| url | https://hdl.handle.net/10568/115873 |
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