Carotenoid crystal formation in Arabidopsis and carrot roots caused by increased phytoene synthase protein levels

As the first pathway-specific enzyme in carotenoid biosynthesis, phytoene synthase (PSY) is a prime regulatory target. This includes a number of biotechnological approaches that have successfully increased the carotenoid content in agronomically relevant non-green plant tissues through tissue-specif...

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Bibliographic Details
Main Authors: Maass, Dirk, Arango, Jacobo, Wüst, Florian, Beyer, Peter, Welsch, Ralf
Format: Journal Article
Language:Inglés
Published: Public Library of Science 2009
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Online Access:https://hdl.handle.net/10568/115369
Description
Summary:As the first pathway-specific enzyme in carotenoid biosynthesis, phytoene synthase (PSY) is a prime regulatory target. This includes a number of biotechnological approaches that have successfully increased the carotenoid content in agronomically relevant non-green plant tissues through tissue-specific PSY overexpression. We investigated the differential effects of constitutive AtPSY overexpression in green and non-green cells of transgenic Arabidopsis lines. This revealed striking similarities to the situation found in orange carrot roots with respect to carotenoid amounts and sequestration mechanism.