Founder events influence structures of Aspergillus flavus populations

In warm regions, agricultural fields are occupied by complex Aspergillus flavus communities composed of isolates in many vegetative compatibility groups (VCGs) with varying abilities to produce highly toxic, carcinogenic aflatoxins. Aflatoxin contamination is reduced with biocontrol products that en...

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Autores principales: Ortega‐Beltran, A., Callicott, K.A., Cotty, P.J.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Wiley 2020
Materias:
Acceso en línea:https://hdl.handle.net/10568/114956
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author Ortega‐Beltran, A.
Callicott, K.A.
Cotty, P.J.
author_browse Callicott, K.A.
Cotty, P.J.
Ortega‐Beltran, A.
author_facet Ortega‐Beltran, A.
Callicott, K.A.
Cotty, P.J.
author_sort Ortega‐Beltran, A.
collection Repository of Agricultural Research Outputs (CGSpace)
description In warm regions, agricultural fields are occupied by complex Aspergillus flavus communities composed of isolates in many vegetative compatibility groups (VCGs) with varying abilities to produce highly toxic, carcinogenic aflatoxins. Aflatoxin contamination is reduced with biocontrol products that enable atoxigenic isolates from atoxigenic VCGs to dominate the population. Shifts in VCG frequencies similar to those caused by the introduction of biocontrol isolates were detected in Sonora, Mexico, where biocontrol is not currently practiced. The shifts were attributed to founder events. Although VCGs reproduce clonally, significant diversity exists within VCGs. Simple sequence repeat (SSR) fingerprinting revealed that increased frequencies of VCG YV150 involved a single haplotype. This is consistent with a founder event. Additionally, great diversity was detected among 82 YV150 isolates collected over 20 years across Mexico and the United States. Thirty‐six YV150 haplotypes were separated into two populations by Structure and SplitsTree analyses. Sixty‐five percent of isolates had MAT1‐1 and belonged to one population. The remaining had MAT1‐2 and belonged to the second population. SSR alleles varied within populations, but recombination between populations was not detected despite co‐occurrence at some locations. Results suggest that YV150 isolates with opposite mating‐type have either strongly restrained or lost sexual reproduction among themselves.
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spelling CGSpace1149562025-11-11T11:07:12Z Founder events influence structures of Aspergillus flavus populations Ortega‐Beltran, A. Callicott, K.A. Cotty, P.J. aspergillus flavus aflatoxins maize biological control In warm regions, agricultural fields are occupied by complex Aspergillus flavus communities composed of isolates in many vegetative compatibility groups (VCGs) with varying abilities to produce highly toxic, carcinogenic aflatoxins. Aflatoxin contamination is reduced with biocontrol products that enable atoxigenic isolates from atoxigenic VCGs to dominate the population. Shifts in VCG frequencies similar to those caused by the introduction of biocontrol isolates were detected in Sonora, Mexico, where biocontrol is not currently practiced. The shifts were attributed to founder events. Although VCGs reproduce clonally, significant diversity exists within VCGs. Simple sequence repeat (SSR) fingerprinting revealed that increased frequencies of VCG YV150 involved a single haplotype. This is consistent with a founder event. Additionally, great diversity was detected among 82 YV150 isolates collected over 20 years across Mexico and the United States. Thirty‐six YV150 haplotypes were separated into two populations by Structure and SplitsTree analyses. Sixty‐five percent of isolates had MAT1‐1 and belonged to one population. The remaining had MAT1‐2 and belonged to the second population. SSR alleles varied within populations, but recombination between populations was not detected despite co‐occurrence at some locations. Results suggest that YV150 isolates with opposite mating‐type have either strongly restrained or lost sexual reproduction among themselves. 2020-08 2021-09-13T14:27:44Z 2021-09-13T14:27:44Z Journal Article https://hdl.handle.net/10568/114956 en Open Access application/pdf Wiley Ortega‐Beltran, A., Callicott, K.A. & Cotty, P.J. (2020). Founder events influence structures of Aspergillus flavus populations. Environmental Microbiology, 22(8), 3522-3534.
spellingShingle aspergillus flavus
aflatoxins
maize
biological control
Ortega‐Beltran, A.
Callicott, K.A.
Cotty, P.J.
Founder events influence structures of Aspergillus flavus populations
title Founder events influence structures of Aspergillus flavus populations
title_full Founder events influence structures of Aspergillus flavus populations
title_fullStr Founder events influence structures of Aspergillus flavus populations
title_full_unstemmed Founder events influence structures of Aspergillus flavus populations
title_short Founder events influence structures of Aspergillus flavus populations
title_sort founder events influence structures of aspergillus flavus populations
topic aspergillus flavus
aflatoxins
maize
biological control
url https://hdl.handle.net/10568/114956
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