Serological survey and metagenomic discovery of potato viruses in Rwanda and Burundi reveals absence of PVY in Burundi and first report of TRV in potatoes in sub-Saharan Africa
Worldwide, potato (Solanum tuberosum L.) is the third most important food crop after rice and wheat. Its production is however constrained by several virus diseases. The occurrence and distribution of the economically important viruses and associated insect vectors is however not known for Rwanda an...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/114945 |
| _version_ | 1855542984593899520 |
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| author | Okonya, J. Gamarra, H. Nduwayezu, A. Bararyenya, A. Kroschel, Jürgen Kreuze, Jan F. |
| author_browse | Bararyenya, A. Gamarra, H. Kreuze, Jan F. Kroschel, Jürgen Nduwayezu, A. Okonya, J. |
| author_facet | Okonya, J. Gamarra, H. Nduwayezu, A. Bararyenya, A. Kroschel, Jürgen Kreuze, Jan F. |
| author_sort | Okonya, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Worldwide, potato (Solanum tuberosum L.) is the third most important food crop after rice and wheat. Its production is however constrained by several virus diseases. The occurrence and distribution of the economically important viruses and associated insect vectors is however not known for Rwanda and Burundi, where potato is an important food security and income crop. We surveyed 194 potato fields for viruses and insect vectors. Aphids were commonly found infesting farmers' potato fields in contrast to whiteflies. Testing by Enzyme Linked Immunosorbent Assay (ELISA) for six potato viruses identified five viruses: potato leafroll virus (PLRV), potato virus X, S, M and Y (PVX, PVS, PVM, PVY) in Rwanda and two viruses (PLRV and PVS) in Burundi. A subset of samples were analyzed using small RNA sequencing and assembly (sRSA) and additionally revealed presence of PVX and for the first time, tobacco rattle virus (TRV) in Burundi. PLRV and PVS were most common while PVY was rare and not found in Burundi, which is highly unusual. To our knowledge, this is the first report of TRV infecting potatoes in sub-Saharan Africa. Phylogenetic analysis of 14 complete viral genomes determined by sRSA suggested multiple introductions of viruses into the region. |
| format | Journal Article |
| id | CGSpace114945 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1149452024-11-14T08:05:43Z Serological survey and metagenomic discovery of potato viruses in Rwanda and Burundi reveals absence of PVY in Burundi and first report of TRV in potatoes in sub-Saharan Africa Okonya, J. Gamarra, H. Nduwayezu, A. Bararyenya, A. Kroschel, Jürgen Kreuze, Jan F. solanum tuberosum aphidoidea viroses potatoes Worldwide, potato (Solanum tuberosum L.) is the third most important food crop after rice and wheat. Its production is however constrained by several virus diseases. The occurrence and distribution of the economically important viruses and associated insect vectors is however not known for Rwanda and Burundi, where potato is an important food security and income crop. We surveyed 194 potato fields for viruses and insect vectors. Aphids were commonly found infesting farmers' potato fields in contrast to whiteflies. Testing by Enzyme Linked Immunosorbent Assay (ELISA) for six potato viruses identified five viruses: potato leafroll virus (PLRV), potato virus X, S, M and Y (PVX, PVS, PVM, PVY) in Rwanda and two viruses (PLRV and PVS) in Burundi. A subset of samples were analyzed using small RNA sequencing and assembly (sRSA) and additionally revealed presence of PVX and for the first time, tobacco rattle virus (TRV) in Burundi. PLRV and PVS were most common while PVY was rare and not found in Burundi, which is highly unusual. To our knowledge, this is the first report of TRV infecting potatoes in sub-Saharan Africa. Phylogenetic analysis of 14 complete viral genomes determined by sRSA suggested multiple introductions of viruses into the region. 2021-09 2021-09-10T05:33:42Z 2021-09-10T05:33:42Z Journal Article https://hdl.handle.net/10568/114945 en Limited Access Elsevier Okonya, J. S., Gamarra, H., Nduwayezu, A., Bararyenya, A., Kroschel, J., & Kreuze, J. (2021). Serological survey and metagenomic discovery of potato viruses in Rwanda and Burundi reveals absence of PVY in Burundi and first report of TRV in potatoes in sub-Saharan Africa. Virus Research. ISSN 1872-7492. 302, 198487 |
| spellingShingle | solanum tuberosum aphidoidea viroses potatoes Okonya, J. Gamarra, H. Nduwayezu, A. Bararyenya, A. Kroschel, Jürgen Kreuze, Jan F. Serological survey and metagenomic discovery of potato viruses in Rwanda and Burundi reveals absence of PVY in Burundi and first report of TRV in potatoes in sub-Saharan Africa |
| title | Serological survey and metagenomic discovery of potato viruses in Rwanda and Burundi reveals absence of PVY in Burundi and first report of TRV in potatoes in sub-Saharan Africa |
| title_full | Serological survey and metagenomic discovery of potato viruses in Rwanda and Burundi reveals absence of PVY in Burundi and first report of TRV in potatoes in sub-Saharan Africa |
| title_fullStr | Serological survey and metagenomic discovery of potato viruses in Rwanda and Burundi reveals absence of PVY in Burundi and first report of TRV in potatoes in sub-Saharan Africa |
| title_full_unstemmed | Serological survey and metagenomic discovery of potato viruses in Rwanda and Burundi reveals absence of PVY in Burundi and first report of TRV in potatoes in sub-Saharan Africa |
| title_short | Serological survey and metagenomic discovery of potato viruses in Rwanda and Burundi reveals absence of PVY in Burundi and first report of TRV in potatoes in sub-Saharan Africa |
| title_sort | serological survey and metagenomic discovery of potato viruses in rwanda and burundi reveals absence of pvy in burundi and first report of trv in potatoes in sub saharan africa |
| topic | solanum tuberosum aphidoidea viroses potatoes |
| url | https://hdl.handle.net/10568/114945 |
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