Proteo-trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae)
Meteorus pulchricornis (Ichneumonoidea, Braconidae) is an endoparasitoid wasp of lepidopteran caterpillars. Its parasitic success relies on vesicles (named M. pulchricornis Virus-Like Particles or MpVLPs) that are synthesized in the venom gland and injected into the parasitoid host along with the ve...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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MDPI
2021
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/114556 |
| _version_ | 1855521511706722304 |
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| author | Gatti, J.L. Belghazi, M. Legeai, F. Ravallec, M. Frayssinet, M. Robin, S. Aboubakar-Souna, D. Srinivasan, R. Tamo, Manuele Poirie, M. Volkoff, A.N. |
| author_browse | Aboubakar-Souna, D. Belghazi, M. Frayssinet, M. Gatti, J.L. Legeai, F. Poirie, M. Ravallec, M. Robin, S. Srinivasan, R. Tamo, Manuele Volkoff, A.N. |
| author_facet | Gatti, J.L. Belghazi, M. Legeai, F. Ravallec, M. Frayssinet, M. Robin, S. Aboubakar-Souna, D. Srinivasan, R. Tamo, Manuele Poirie, M. Volkoff, A.N. |
| author_sort | Gatti, J.L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Meteorus pulchricornis (Ichneumonoidea, Braconidae) is an endoparasitoid wasp of lepidopteran caterpillars. Its parasitic success relies on vesicles (named M. pulchricornis Virus-Like Particles or MpVLPs) that are synthesized in the venom gland and injected into the parasitoid host along with the venom during oviposition. In order to define the content and understand the biogenesis of these atypical vesicles, we performed a transcriptome analysis of the venom gland and a proteomic analysis of the venom and purified MpVLPs. About half of the MpVLPs and soluble venom proteins identified were unknown and no similarity with any known viral sequence was found. However, MpVLPs contained a large number of proteins labelled as metalloproteinases while the most abundant protein family in the soluble venom was that of proteins containing the Domain of Unknown Function DUF-4803. The high number of these proteins identified suggests that a large expansion of these two protein families occurred in M. pulchricornis. Therefore, although the exact mechanism of MpVLPs formation remains to be elucidated, these vesicles appear to be “metalloproteinase bombs” that may have several physiological roles in the host including modifying the functions of its immune cells. The role of DUF4803 proteins, also present in the venom of other braconids, remains to be clarified. |
| format | Journal Article |
| id | CGSpace114556 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1145562025-11-11T10:11:53Z Proteo-trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae) Gatti, J.L. Belghazi, M. Legeai, F. Ravallec, M. Frayssinet, M. Robin, S. Aboubakar-Souna, D. Srinivasan, R. Tamo, Manuele Poirie, M. Volkoff, A.N. meteorus insects pests of plants pest insects braconidae proteomic transcriptome metalloproteins venoms Meteorus pulchricornis (Ichneumonoidea, Braconidae) is an endoparasitoid wasp of lepidopteran caterpillars. Its parasitic success relies on vesicles (named M. pulchricornis Virus-Like Particles or MpVLPs) that are synthesized in the venom gland and injected into the parasitoid host along with the venom during oviposition. In order to define the content and understand the biogenesis of these atypical vesicles, we performed a transcriptome analysis of the venom gland and a proteomic analysis of the venom and purified MpVLPs. About half of the MpVLPs and soluble venom proteins identified were unknown and no similarity with any known viral sequence was found. However, MpVLPs contained a large number of proteins labelled as metalloproteinases while the most abundant protein family in the soluble venom was that of proteins containing the Domain of Unknown Function DUF-4803. The high number of these proteins identified suggests that a large expansion of these two protein families occurred in M. pulchricornis. Therefore, although the exact mechanism of MpVLPs formation remains to be elucidated, these vesicles appear to be “metalloproteinase bombs” that may have several physiological roles in the host including modifying the functions of its immune cells. The role of DUF4803 proteins, also present in the venom of other braconids, remains to be clarified. 2021 2021-08-04T10:41:18Z 2021-08-04T10:41:18Z Journal Article https://hdl.handle.net/10568/114556 en Open Access application/pdf MDPI Gatti, J.L., Belghazi, M., Legeai, F., Ravallec, M., Frayssinet, M., Robin, S., ... & Volkoff, A.N. (2021). Proteo-trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae). Toxins, 13(7), 502: 1-37. |
| spellingShingle | meteorus insects pests of plants pest insects braconidae proteomic transcriptome metalloproteins venoms Gatti, J.L. Belghazi, M. Legeai, F. Ravallec, M. Frayssinet, M. Robin, S. Aboubakar-Souna, D. Srinivasan, R. Tamo, Manuele Poirie, M. Volkoff, A.N. Proteo-trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae) |
| title | Proteo-trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae) |
| title_full | Proteo-trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae) |
| title_fullStr | Proteo-trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae) |
| title_full_unstemmed | Proteo-trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae) |
| title_short | Proteo-trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae) |
| title_sort | proteo trancriptomic analyses reveal a large expansion of metalloprotease like proteins in atypical venom vesicles of the wasp meteorus pulchricornis braconidae |
| topic | meteorus insects pests of plants pest insects braconidae proteomic transcriptome metalloproteins venoms |
| url | https://hdl.handle.net/10568/114556 |
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