Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat

Meat from wildlife species (bushmeat) represents a major source of dietary protein in low- and middle-income countries where humans and wildlife live in close proximity. Despite the occurrence of zoonotic pathogens in wildlife, their prevalence in bushmeat remains unknown. To assess the risk of expo...

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Autores principales: Katani, R., Schilling, M.A., Lyimo, B., Eblate, E., Martin, A., Tonui, Triza, Cattadori, I.M., Francesconi, S.C., Estes, A.B., Rentsch, D., Srinivasan, S., Lyimo, S., Munuo, L., Tiambo, Christian K., Stomeo, Francesca, Gwakisa, P.S., Mosha, F., Hudson, P.J., Buza, J.J., Kapur, V.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2021
Materias:
Acceso en línea:https://hdl.handle.net/10568/114463
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author Katani, R.
Schilling, M.A.
Lyimo, B.
Eblate, E.
Martin, A.
Tonui, Triza
Cattadori, I.M.
Francesconi, S.C.
Estes, A.B.
Rentsch, D.
Srinivasan, S.
Lyimo, S.
Munuo, L.
Tiambo, Christian K.
Stomeo, Francesca
Gwakisa, P.S.
Mosha, F.
Hudson, P.J.
Buza, J.J.
Kapur, V.
author_browse Buza, J.J.
Cattadori, I.M.
Eblate, E.
Estes, A.B.
Francesconi, S.C.
Gwakisa, P.S.
Hudson, P.J.
Kapur, V.
Katani, R.
Lyimo, B.
Lyimo, S.
Martin, A.
Mosha, F.
Munuo, L.
Rentsch, D.
Schilling, M.A.
Srinivasan, S.
Stomeo, Francesca
Tiambo, Christian K.
Tonui, Triza
author_facet Katani, R.
Schilling, M.A.
Lyimo, B.
Eblate, E.
Martin, A.
Tonui, Triza
Cattadori, I.M.
Francesconi, S.C.
Estes, A.B.
Rentsch, D.
Srinivasan, S.
Lyimo, S.
Munuo, L.
Tiambo, Christian K.
Stomeo, Francesca
Gwakisa, P.S.
Mosha, F.
Hudson, P.J.
Buza, J.J.
Kapur, V.
author_sort Katani, R.
collection Repository of Agricultural Research Outputs (CGSpace)
description Meat from wildlife species (bushmeat) represents a major source of dietary protein in low- and middle-income countries where humans and wildlife live in close proximity. Despite the occurrence of zoonotic pathogens in wildlife, their prevalence in bushmeat remains unknown. To assess the risk of exposure to major pathogens in bushmeat, a total of 3784 samples, both fresh and processed, were collected from three major regions in Tanzania during both rainy and dry seasons, and were screened by real-time PCR for the presence of DNA signatures of Bacillus anthracis (B. anthracis), Brucella spp. (Brucella) and Coxiella burnetii (Coxiella). The analysis identified DNA signatures of B. anthracis (0.48%), Brucella (0.9%), and Coxiella (0.66%) in a total of 77 samples. Highest prevalence rates of B. anthracis, Brucella, and Coxiella were observed in wildebeest (56%), dik-dik (50%), and impala (24%), respectively. Fresh samples, those collected during the rainy season, and samples from Selous or Serengeti had a greater relative risk of being positive. Microbiome characterization identified Firmicutes and Proteobacteria as the most abundant phyla. The results highlight and define potential risks of exposure to endemic wildlife diseases from bushmeat and the need for future investigations to address the public health and emerging infectious disease risks associated with bushmeat harvesting, trade, and consumption.
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spelling CGSpace1144632025-01-24T14:12:45Z Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat Katani, R. Schilling, M.A. Lyimo, B. Eblate, E. Martin, A. Tonui, Triza Cattadori, I.M. Francesconi, S.C. Estes, A.B. Rentsch, D. Srinivasan, S. Lyimo, S. Munuo, L. Tiambo, Christian K. Stomeo, Francesca Gwakisa, P.S. Mosha, F. Hudson, P.J. Buza, J.J. Kapur, V. animal health health food safety genetics bushmeat zoonoses livestock Meat from wildlife species (bushmeat) represents a major source of dietary protein in low- and middle-income countries where humans and wildlife live in close proximity. Despite the occurrence of zoonotic pathogens in wildlife, their prevalence in bushmeat remains unknown. To assess the risk of exposure to major pathogens in bushmeat, a total of 3784 samples, both fresh and processed, were collected from three major regions in Tanzania during both rainy and dry seasons, and were screened by real-time PCR for the presence of DNA signatures of Bacillus anthracis (B. anthracis), Brucella spp. (Brucella) and Coxiella burnetii (Coxiella). The analysis identified DNA signatures of B. anthracis (0.48%), Brucella (0.9%), and Coxiella (0.66%) in a total of 77 samples. Highest prevalence rates of B. anthracis, Brucella, and Coxiella were observed in wildebeest (56%), dik-dik (50%), and impala (24%), respectively. Fresh samples, those collected during the rainy season, and samples from Selous or Serengeti had a greater relative risk of being positive. Microbiome characterization identified Firmicutes and Proteobacteria as the most abundant phyla. The results highlight and define potential risks of exposure to endemic wildlife diseases from bushmeat and the need for future investigations to address the public health and emerging infectious disease risks associated with bushmeat harvesting, trade, and consumption. 2021-06-23 2021-07-29T14:29:22Z 2021-07-29T14:29:22Z Journal Article https://hdl.handle.net/10568/114463 en Open Access Springer Katani, R., Schilling, M.A., Lyimo, B., Eblate, E., Martin, A., Tonui, T., Cattadori, I.M., Francesconi, S.C., Estes, A.B., Rentsch, D., Srinivasan, S., Lyimo, S., Munuo, L., Tiambo, C.K., Stomeo, F., Gwakisa, P., Mosha, F., Hudson, P.J., Buza, J.J. and Kapur, V. 2021. Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat. Scientific reports 11:14876.
spellingShingle animal health
health
food safety
genetics
bushmeat
zoonoses
livestock
Katani, R.
Schilling, M.A.
Lyimo, B.
Eblate, E.
Martin, A.
Tonui, Triza
Cattadori, I.M.
Francesconi, S.C.
Estes, A.B.
Rentsch, D.
Srinivasan, S.
Lyimo, S.
Munuo, L.
Tiambo, Christian K.
Stomeo, Francesca
Gwakisa, P.S.
Mosha, F.
Hudson, P.J.
Buza, J.J.
Kapur, V.
Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
title Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
title_full Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
title_fullStr Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
title_full_unstemmed Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
title_short Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat
title_sort identification of bacillus anthracis brucella spp and coxiella burnetii dna signatures from bushmeat
topic animal health
health
food safety
genetics
bushmeat
zoonoses
livestock
url https://hdl.handle.net/10568/114463
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