An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems
Taro (Colocasia esculenta L. Schott) is an important crop for food and nutrition security, incomes, and livelihood of people in developing countries. Its cultivation and productivity have been limited mainly due to a lack of quality planting materials. Therefore, this study developed an effective mi...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Academic Journals
2021
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/114461 |
| _version_ | 1855539555827974144 |
|---|---|
| author | Kepue, M.S. Mweu, C.M. Kariuki, D.M. Kerubo, L. Njaci, Isaac Njoroge, Anne Kago, Leah Mware, B.O. Ghimire, Sita R. |
| author_browse | Ghimire, Sita R. Kago, Leah Kariuki, D.M. Kepue, M.S. Kerubo, L. Mware, B.O. Mweu, C.M. Njaci, Isaac Njoroge, Anne |
| author_facet | Kepue, M.S. Mweu, C.M. Kariuki, D.M. Kerubo, L. Njaci, Isaac Njoroge, Anne Kago, Leah Mware, B.O. Ghimire, Sita R. |
| author_sort | Kepue, M.S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Taro (Colocasia esculenta L. Schott) is an important crop for food and nutrition security, incomes, and livelihood of people in developing countries. Its cultivation and productivity have been limited mainly due to a lack of quality planting materials. Therefore, this study developed an effective micropropagation method for two common taro varieties in Kenya (Purple Wild and Dasheen) using apical meristem derived from portions of the corm and base of leaf petioles. This study optimized the 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) levels for a better shoot and root development. The highest shoot induction was observed in both varieties when Murashige & Skoog (MS) media was supplemented with 2 mg/L of BAP. Similarly, the maximum rooting response was achieved in both varieties on half-strength MS media supplemented with 0.5 mg/L of IBA. The study also revealed significant interactions between variety × BAP levels and variety × IBA levels (P<0.0001) for shoot and root development, respectively. This apical meristem-based micropropagation method developed in this study can be used for rapid multiplication of genetically clean planting materials for commercial uses and to establish taro transformation protocols. |
| format | Journal Article |
| id | CGSpace114461 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Academic Journals |
| publisherStr | Academic Journals |
| record_format | dspace |
| spelling | CGSpace1144612024-10-03T07:40:58Z An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems Kepue, M.S. Mweu, C.M. Kariuki, D.M. Kerubo, L. Njaci, Isaac Njoroge, Anne Kago, Leah Mware, B.O. Ghimire, Sita R. crop improvement livelihoods crops biotechnology Taro (Colocasia esculenta L. Schott) is an important crop for food and nutrition security, incomes, and livelihood of people in developing countries. Its cultivation and productivity have been limited mainly due to a lack of quality planting materials. Therefore, this study developed an effective micropropagation method for two common taro varieties in Kenya (Purple Wild and Dasheen) using apical meristem derived from portions of the corm and base of leaf petioles. This study optimized the 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) levels for a better shoot and root development. The highest shoot induction was observed in both varieties when Murashige & Skoog (MS) media was supplemented with 2 mg/L of BAP. Similarly, the maximum rooting response was achieved in both varieties on half-strength MS media supplemented with 0.5 mg/L of IBA. The study also revealed significant interactions between variety × BAP levels and variety × IBA levels (P<0.0001) for shoot and root development, respectively. This apical meristem-based micropropagation method developed in this study can be used for rapid multiplication of genetically clean planting materials for commercial uses and to establish taro transformation protocols. 2021-07-31 2021-07-29T14:29:14Z 2021-07-29T14:29:14Z Journal Article https://hdl.handle.net/10568/114461 en Open Access Academic Journals Kepue, M.S., Mweu, C.M., Kariuki, D.M., Kerubo, L., Njaci, I., Njoroge, A., Kago, L., Mware, B.O. and Ghimire, S.R. 2021. An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems. African Journal of Biotechnology 20(7):293-299. |
| spellingShingle | crop improvement livelihoods crops biotechnology Kepue, M.S. Mweu, C.M. Kariuki, D.M. Kerubo, L. Njaci, Isaac Njoroge, Anne Kago, Leah Mware, B.O. Ghimire, Sita R. An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems |
| title | An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems |
| title_full | An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems |
| title_fullStr | An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems |
| title_full_unstemmed | An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems |
| title_short | An efficient in-vitro regeneration system of Taro (Colocasia esculenta L. Schott) using apical meristems |
| title_sort | efficient in vitro regeneration system of taro colocasia esculenta l schott using apical meristems |
| topic | crop improvement livelihoods crops biotechnology |
| url | https://hdl.handle.net/10568/114461 |
| work_keys_str_mv | AT kepuems anefficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT mweucm anefficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT kariukidm anefficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT kerubol anefficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT njaciisaac anefficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT njorogeanne anefficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT kagoleah anefficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT mwarebo anefficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT ghimiresitar anefficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT kepuems efficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT mweucm efficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT kariukidm efficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT kerubol efficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT njaciisaac efficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT njorogeanne efficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT kagoleah efficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT mwarebo efficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems AT ghimiresitar efficientinvitroregenerationsystemoftarocolocasiaesculentalschottusingapicalmeristems |