Occurrence of a novel strain of Moroccan watermelon mosaic virus infecting pumpkins in Kenya
The Potyvirus Moroccan watermelon mosaic virus (MWMV) naturally infects and severely threatens production of cucurbits and papaya. In this study, we identified and characterized MWMV isolated from pumpkin (Cucurbita moschata) intercropped with MWMV-infected papaya plants through next-generation sequ...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Scientific Societies
2022
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| Acceso en línea: | https://hdl.handle.net/10568/114367 |
| _version_ | 1855521393167302656 |
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| author | Mumo, N. Ateka, E.M. Mamati, E. Rimberia, F.K. Asudi, G.O. Machuka, Eunice M. Juguna, J.N. Stomeo, Francesca Pelle, Roger |
| author_browse | Asudi, G.O. Ateka, E.M. Juguna, J.N. Machuka, Eunice M. Mamati, E. Mumo, N. Pelle, Roger Rimberia, F.K. Stomeo, Francesca |
| author_facet | Mumo, N. Ateka, E.M. Mamati, E. Rimberia, F.K. Asudi, G.O. Machuka, Eunice M. Juguna, J.N. Stomeo, Francesca Pelle, Roger |
| author_sort | Mumo, N. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The Potyvirus Moroccan watermelon mosaic virus (MWMV) naturally infects and severely threatens production of cucurbits and papaya. In this study, we identified and characterized MWMV isolated from pumpkin (Cucurbita moschata) intercropped with MWMV-infected papaya plants through next-generation sequencing (NGS) and Sanger sequencing approaches. Complete MWMV genome sequences were obtained from two pumpkin samples through NGS and validated using Sanger sequencing. The isolates shared 83.4 to 83.7% nucleotide (nt) and 92.3 to 95.1% amino acid (aa) sequence identities in the coat protein and 79.5 to 79.9% nt and 89.2 to 89.7% aa identities in the polyprotein with papaya isolates of MWMV. Phylogenetic analysis using complete polyprotein nt sequences revealed the clustering of both pumpkin isolates of MWMV with corresponding sequences of cucurbit isolates of the virus from other parts of Africa and the Mediterranean regions, distinct from a clade formed by papaya isolates. Through sap inoculation, a pumpkin isolate of MWMV was pathogenic on zucchini (Cucurbita pepo), watermelon (Citrullus lanatus), and cucumber (Cucumis sativus) but not on papaya. Conversely, the papaya isolate of MWMV was nonpathogenic on pumpkin, watermelon, and cucumber, but it infected zucchini. The results suggest the occurrence of two strains of MWMV in Kenya having different biological characteristics associated with the host specificity.[Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license . |
| format | Journal Article |
| id | CGSpace114367 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace1143672025-12-02T10:59:51Z Occurrence of a novel strain of Moroccan watermelon mosaic virus infecting pumpkins in Kenya Mumo, N. Ateka, E.M. Mamati, E. Rimberia, F.K. Asudi, G.O. Machuka, Eunice M. Juguna, J.N. Stomeo, Francesca Pelle, Roger plant diseases crops The Potyvirus Moroccan watermelon mosaic virus (MWMV) naturally infects and severely threatens production of cucurbits and papaya. In this study, we identified and characterized MWMV isolated from pumpkin (Cucurbita moschata) intercropped with MWMV-infected papaya plants through next-generation sequencing (NGS) and Sanger sequencing approaches. Complete MWMV genome sequences were obtained from two pumpkin samples through NGS and validated using Sanger sequencing. The isolates shared 83.4 to 83.7% nucleotide (nt) and 92.3 to 95.1% amino acid (aa) sequence identities in the coat protein and 79.5 to 79.9% nt and 89.2 to 89.7% aa identities in the polyprotein with papaya isolates of MWMV. Phylogenetic analysis using complete polyprotein nt sequences revealed the clustering of both pumpkin isolates of MWMV with corresponding sequences of cucurbit isolates of the virus from other parts of Africa and the Mediterranean regions, distinct from a clade formed by papaya isolates. Through sap inoculation, a pumpkin isolate of MWMV was pathogenic on zucchini (Cucurbita pepo), watermelon (Citrullus lanatus), and cucumber (Cucumis sativus) but not on papaya. Conversely, the papaya isolate of MWMV was nonpathogenic on pumpkin, watermelon, and cucumber, but it infected zucchini. The results suggest the occurrence of two strains of MWMV in Kenya having different biological characteristics associated with the host specificity.[Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license . 2022-01-01 2021-07-26T08:11:30Z 2021-07-26T08:11:30Z Journal Article https://hdl.handle.net/10568/114367 en Open Access Scientific Societies Mumo, N., Ateka, E.M., Mamati, E., Rimberia, F.K., Asudi, G.O., Machuka, E., Juguna, J.N., Stomeo, F. and Pelle, R. 2021. Occurrence of a novel strain of Moroccan watermelon mosaic virus infecting pumpkins in Kenya. Plant Diseases |
| spellingShingle | plant diseases crops Mumo, N. Ateka, E.M. Mamati, E. Rimberia, F.K. Asudi, G.O. Machuka, Eunice M. Juguna, J.N. Stomeo, Francesca Pelle, Roger Occurrence of a novel strain of Moroccan watermelon mosaic virus infecting pumpkins in Kenya |
| title | Occurrence of a novel strain of Moroccan watermelon mosaic virus infecting pumpkins in Kenya |
| title_full | Occurrence of a novel strain of Moroccan watermelon mosaic virus infecting pumpkins in Kenya |
| title_fullStr | Occurrence of a novel strain of Moroccan watermelon mosaic virus infecting pumpkins in Kenya |
| title_full_unstemmed | Occurrence of a novel strain of Moroccan watermelon mosaic virus infecting pumpkins in Kenya |
| title_short | Occurrence of a novel strain of Moroccan watermelon mosaic virus infecting pumpkins in Kenya |
| title_sort | occurrence of a novel strain of moroccan watermelon mosaic virus infecting pumpkins in kenya |
| topic | plant diseases crops |
| url | https://hdl.handle.net/10568/114367 |
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