Escherichia coli O157:H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern
Escherichia coli O157:H7 is an important foodborne pathogen but largely under investigated in Africa. The objectives of this study were to estimate the prevalence and pattern of antimicrobial resistance of E. coli O157:H7 in lettuce in Addis Ababa, Ethiopia. A total of 390 retail lettuce samples wer...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2021
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| Acceso en línea: | https://hdl.handle.net/10568/114326 |
| _version_ | 1855522534688030720 |
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| author | Haile, A.F. Alonso, Silvia Berhe, N. Atoma, T.B. Boyaka, P.N. Grace, Delia |
| author_browse | Alonso, Silvia Atoma, T.B. Berhe, N. Boyaka, P.N. Grace, Delia Haile, A.F. |
| author_facet | Haile, A.F. Alonso, Silvia Berhe, N. Atoma, T.B. Boyaka, P.N. Grace, Delia |
| author_sort | Haile, A.F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Escherichia coli O157:H7 is an important foodborne pathogen but largely under investigated in Africa. The objectives of this study were to estimate the prevalence and pattern of antimicrobial resistance of E. coli O157:H7 in lettuce in Addis Ababa, Ethiopia. A total of 390 retail lettuce samples were collected across the 10 subcities of Addis Ababa. E. coli O157:H7 was isolated and identified following ISO-16654:2001 standard. The isolates were further tested for antimicrobial susceptibility to 13 antimicrobials using the Kirby–Bauer disk diffusion method. Out of the 390 lettuce samples examined, two (0.51%) carried E. coli O157:H7. The antimicrobial susceptibility pattern of strains showed resistance to ampicillin (100%) and tetracycline (50.0%). One of the two isolates was multidrug resistant to two antimicrobials tested. The results of this study demonstrate the presence of drug-resistant E. coli O157:H7 in lettuce in markets in Addis Ababa. Despite the low prevalence, its presence in a product that is eaten raw highlights potential public health risk in the area associated with this pathogen. |
| format | Journal Article |
| id | CGSpace114326 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1143262025-08-15T13:21:41Z Escherichia coli O157:H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern Haile, A.F. Alonso, Silvia Berhe, N. Atoma, T.B. Boyaka, P.N. Grace, Delia food safety antimicrobial resistance Escherichia coli O157:H7 is an important foodborne pathogen but largely under investigated in Africa. The objectives of this study were to estimate the prevalence and pattern of antimicrobial resistance of E. coli O157:H7 in lettuce in Addis Ababa, Ethiopia. A total of 390 retail lettuce samples were collected across the 10 subcities of Addis Ababa. E. coli O157:H7 was isolated and identified following ISO-16654:2001 standard. The isolates were further tested for antimicrobial susceptibility to 13 antimicrobials using the Kirby–Bauer disk diffusion method. Out of the 390 lettuce samples examined, two (0.51%) carried E. coli O157:H7. The antimicrobial susceptibility pattern of strains showed resistance to ampicillin (100%) and tetracycline (50.0%). One of the two isolates was multidrug resistant to two antimicrobials tested. The results of this study demonstrate the presence of drug-resistant E. coli O157:H7 in lettuce in markets in Addis Ababa. Despite the low prevalence, its presence in a product that is eaten raw highlights potential public health risk in the area associated with this pathogen. 2021-07-16 2021-07-19T11:54:30Z 2021-07-19T11:54:30Z Journal Article https://hdl.handle.net/10568/114326 en Open Access Frontiers Media Haile, A.F., Alonso, S., Berhe, N., Atoma, T.B., Boyaka, P.N. and Grace, D. 2021. Escherichia coli O157:H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern. Frontiers in Microbiology 12: 694506. |
| spellingShingle | food safety antimicrobial resistance Haile, A.F. Alonso, Silvia Berhe, N. Atoma, T.B. Boyaka, P.N. Grace, Delia Escherichia coli O157:H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern |
| title | Escherichia coli O157:H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern |
| title_full | Escherichia coli O157:H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern |
| title_fullStr | Escherichia coli O157:H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern |
| title_full_unstemmed | Escherichia coli O157:H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern |
| title_short | Escherichia coli O157:H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern |
| title_sort | escherichia coli o157 h7 in retail lettuce lactuca sativa in addis ababa city magnitude of contamination and antimicrobial susceptibility pattern |
| topic | food safety antimicrobial resistance |
| url | https://hdl.handle.net/10568/114326 |
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