SNP-based genotyping and whole-genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum, causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin
For decades, Xanthomonas vasicola pv. musacearum (Xvm) has been an economically important bacterial pathogen on enset in Ethiopia. Since 2001, Xvm has also been responsible for significant losses to banana crops in several East and Central African countries, with devastating consequences for smallho...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2021
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/114295 |
| _version_ | 1855514138823884800 |
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| author | Nakato, Gloria V. Studholme, David J. Blomme, Guy Grant, Murray Coutinho, Teresa A. Were, Evans M. Wicker, Emmanuel Mahuku, George S. |
| author_browse | Blomme, Guy Coutinho, Teresa A. Grant, Murray Mahuku, George S. Nakato, Gloria V. Studholme, David J. Were, Evans M. Wicker, Emmanuel |
| author_facet | Nakato, Gloria V. Studholme, David J. Blomme, Guy Grant, Murray Coutinho, Teresa A. Were, Evans M. Wicker, Emmanuel Mahuku, George S. |
| author_sort | Nakato, Gloria V. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | For decades, Xanthomonas vasicola pv. musacearum (Xvm) has been an economically important bacterial pathogen on enset in Ethiopia. Since 2001, Xvm has also been responsible for significant losses to banana crops in several East and Central African countries, with devastating consequences for smallholder farmers. Understanding the genetic diversity within Xvm populations is essential for the smart design of transnationally reasoned, durable, and effective management practices. Previous studies have revealed limited genetic diversity in Xvm, with East African isolates from banana each falling into one of two closely related clades previously designated as sublineages SL 1 and SL 2, the former of which had also been detected on banana and enset in Ethiopia. Given the presumed origin of Xvm in Ethiopia, we hypothesized that both clades might be found in that country, along with additional genotypes not seen in Central and East African bananas. Genotyping of 97 isolates and whole-genome sequencing of 15 isolates revealed not only the presence of SL 2 in Ethiopia, but additional diversity beyond SL 1 and SL 2 in four new clades. Moreover, SL 2 was detected in the Democratic Republic of Congo, where previously SL 1 was the only clade reported. These results demonstrate a greater range of genetic diversity among Xvm isolates than previously reported, especially in Ethiopia, and further support the hypothesis that the East/Central Africa xanthomonas wilt epidemic has been caused by a restricted set of genotypes drawn from a highly diverse pathogen pool in Ethiopia. |
| format | Journal Article |
| id | CGSpace114295 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1142952025-11-11T17:39:55Z SNP-based genotyping and whole-genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum, causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin Nakato, Gloria V. Studholme, David J. Blomme, Guy Grant, Murray Coutinho, Teresa A. Were, Evans M. Wicker, Emmanuel Mahuku, George S. ensete ventricosum musa acuminata xanthomonas genomics plant disease control bacterial diseases genetic diversity genómica control de enfermedades de plantas enfermedades bacterianas horticulture genetics For decades, Xanthomonas vasicola pv. musacearum (Xvm) has been an economically important bacterial pathogen on enset in Ethiopia. Since 2001, Xvm has also been responsible for significant losses to banana crops in several East and Central African countries, with devastating consequences for smallholder farmers. Understanding the genetic diversity within Xvm populations is essential for the smart design of transnationally reasoned, durable, and effective management practices. Previous studies have revealed limited genetic diversity in Xvm, with East African isolates from banana each falling into one of two closely related clades previously designated as sublineages SL 1 and SL 2, the former of which had also been detected on banana and enset in Ethiopia. Given the presumed origin of Xvm in Ethiopia, we hypothesized that both clades might be found in that country, along with additional genotypes not seen in Central and East African bananas. Genotyping of 97 isolates and whole-genome sequencing of 15 isolates revealed not only the presence of SL 2 in Ethiopia, but additional diversity beyond SL 1 and SL 2 in four new clades. Moreover, SL 2 was detected in the Democratic Republic of Congo, where previously SL 1 was the only clade reported. These results demonstrate a greater range of genetic diversity among Xvm isolates than previously reported, especially in Ethiopia, and further support the hypothesis that the East/Central Africa xanthomonas wilt epidemic has been caused by a restricted set of genotypes drawn from a highly diverse pathogen pool in Ethiopia. 2021-04 2021-07-15T10:45:47Z 2021-07-15T10:45:47Z Journal Article https://hdl.handle.net/10568/114295 en Open Access application/pdf Wiley Nakato, G.V.; Studholme, D.J.; Blomme, G.; Grant,M.; Coutinho, T.A.; Were, E.M.; Wicker, E.; Mahuku, G. (2021) SNP-based genotyping and whole-genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum, causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin. Plant Pathology 70(3) p. 534-543. ISSN: 0032-0862 |
| spellingShingle | ensete ventricosum musa acuminata xanthomonas genomics plant disease control bacterial diseases genetic diversity genómica control de enfermedades de plantas enfermedades bacterianas horticulture genetics Nakato, Gloria V. Studholme, David J. Blomme, Guy Grant, Murray Coutinho, Teresa A. Were, Evans M. Wicker, Emmanuel Mahuku, George S. SNP-based genotyping and whole-genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum, causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin |
| title | SNP-based genotyping and whole-genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum, causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin |
| title_full | SNP-based genotyping and whole-genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum, causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin |
| title_fullStr | SNP-based genotyping and whole-genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum, causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin |
| title_full_unstemmed | SNP-based genotyping and whole-genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum, causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin |
| title_short | SNP-based genotyping and whole-genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum, causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin |
| title_sort | snp based genotyping and whole genome sequencing reveal previously unknown genetic diversity in xanthomonas vasicola pv musacearum causal agent of banana xanthomonas wilt in its presumed ethiopian origin |
| topic | ensete ventricosum musa acuminata xanthomonas genomics plant disease control bacterial diseases genetic diversity genómica control de enfermedades de plantas enfermedades bacterianas horticulture genetics |
| url | https://hdl.handle.net/10568/114295 |
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