Introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenya
Maize lethal necrosis (MLN), resulting from co-infection by maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) can cause up to 100% yield losses in maize in Africa under serious disease conditions. Maize improvement through conventional backcross (BC) takes many generations but ca...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Frontiers Media
2021
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/114272 |
| _version_ | 1855516136492236800 |
|---|---|
| author | Awata, L.A.O. Ifie, B.E. Danquah, E. Jumbo, M.B. Suresh, L.M. Gowda, M. Marchelo-Dragga, Philip W. Olsen, M.S. Shorinola, Oluwaseyi Yao, Nasser Boddupalli, P.M. Tongoona, P.B. |
| author_browse | Awata, L.A.O. Boddupalli, P.M. Danquah, E. Gowda, M. Ifie, B.E. Jumbo, M.B. Marchelo-Dragga, Philip W. Olsen, M.S. Shorinola, Oluwaseyi Suresh, L.M. Tongoona, P.B. Yao, Nasser |
| author_facet | Awata, L.A.O. Ifie, B.E. Danquah, E. Jumbo, M.B. Suresh, L.M. Gowda, M. Marchelo-Dragga, Philip W. Olsen, M.S. Shorinola, Oluwaseyi Yao, Nasser Boddupalli, P.M. Tongoona, P.B. |
| author_sort | Awata, L.A.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Maize lethal necrosis (MLN), resulting from co-infection by maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) can cause up to 100% yield losses in maize in Africa under serious disease conditions. Maize improvement through conventional backcross (BC) takes many generations but can significantly be shortened when molecular tools are utilized in the breeding process. We used a donor parent (KS23-6) to transfer quantitative trait loci (QTL) for resistance to MLN into nine adapted but MLN susceptible lines. Nurseries were established in Kiboko, Kenya during 2015–2017 seasons and BC3F2 progeny were developed using marker assisted backcrossing (MABC) approach. Six single nucleotide polymorphism (SNP) markers linked to QTL for resistance to MLN were used to genotype 2,400 BC3F2 lines using Kompetitive Allele Specific PCR (KASP) platform. We detected that two of the six QTL had major effects for resistance to MLN under artificial inoculation field conditions in 56 candidate BC3F2 lines. To confirm whether these two QTL are reproducible under different field conditions, the 56 BC3F2 lines including their parents were evaluated in replicated trials for two seasons under artificial MLN inoculations in Naivasha, Kenya in 2018. Strong association of genotype with phenotype was detected. Consequently, 19 superior BC3F2 lines with favorable alleles and showing improved levels of resistance to MLN under artificial field inoculation were identified. These elite lines represent superior genetic resources for improvement of maize hybrids for resistance to MLN. However, 20 BC3F2 lines were fixed for both KASP markers but were susceptible to MLN under field conditions, which could suggest weak linkage between the KASP markers and target genes. The validated two major QTL can be utilized to speed up the breeding process but additional loci need to be identified between the KASP markers and the resistance genes to strengthen the linkage. |
| format | Journal Article |
| id | CGSpace114272 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1142722024-05-01T08:15:24Z Introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenya Awata, L.A.O. Ifie, B.E. Danquah, E. Jumbo, M.B. Suresh, L.M. Gowda, M. Marchelo-Dragga, Philip W. Olsen, M.S. Shorinola, Oluwaseyi Yao, Nasser Boddupalli, P.M. Tongoona, P.B. crops maize research food systems Maize lethal necrosis (MLN), resulting from co-infection by maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) can cause up to 100% yield losses in maize in Africa under serious disease conditions. Maize improvement through conventional backcross (BC) takes many generations but can significantly be shortened when molecular tools are utilized in the breeding process. We used a donor parent (KS23-6) to transfer quantitative trait loci (QTL) for resistance to MLN into nine adapted but MLN susceptible lines. Nurseries were established in Kiboko, Kenya during 2015–2017 seasons and BC3F2 progeny were developed using marker assisted backcrossing (MABC) approach. Six single nucleotide polymorphism (SNP) markers linked to QTL for resistance to MLN were used to genotype 2,400 BC3F2 lines using Kompetitive Allele Specific PCR (KASP) platform. We detected that two of the six QTL had major effects for resistance to MLN under artificial inoculation field conditions in 56 candidate BC3F2 lines. To confirm whether these two QTL are reproducible under different field conditions, the 56 BC3F2 lines including their parents were evaluated in replicated trials for two seasons under artificial MLN inoculations in Naivasha, Kenya in 2018. Strong association of genotype with phenotype was detected. Consequently, 19 superior BC3F2 lines with favorable alleles and showing improved levels of resistance to MLN under artificial field inoculation were identified. These elite lines represent superior genetic resources for improvement of maize hybrids for resistance to MLN. However, 20 BC3F2 lines were fixed for both KASP markers but were susceptible to MLN under field conditions, which could suggest weak linkage between the KASP markers and target genes. The validated two major QTL can be utilized to speed up the breeding process but additional loci need to be identified between the KASP markers and the resistance genes to strengthen the linkage. 2021-05-03 2021-07-14T09:45:48Z 2021-07-14T09:45:48Z Journal Article https://hdl.handle.net/10568/114272 en Open Access Frontiers Media Awata, L.A.O., Ifie, B.E., Danquah, E., Jumbo, M.B., Suresh, L.M., Gowda, M., Marchelo-Dragga, P.W., Olsen, M.S., Shorinola, O., Yao, N.K., Boddupalli, P.M. and Tongoona, P.B. 2021. Introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenya. Frontiers in Plant Science 12:649308. |
| spellingShingle | crops maize research food systems Awata, L.A.O. Ifie, B.E. Danquah, E. Jumbo, M.B. Suresh, L.M. Gowda, M. Marchelo-Dragga, Philip W. Olsen, M.S. Shorinola, Oluwaseyi Yao, Nasser Boddupalli, P.M. Tongoona, P.B. Introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenya |
| title | Introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenya |
| title_full | Introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenya |
| title_fullStr | Introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenya |
| title_full_unstemmed | Introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenya |
| title_short | Introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenya |
| title_sort | introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in naivasha kenya |
| topic | crops maize research food systems |
| url | https://hdl.handle.net/10568/114272 |
| work_keys_str_mv | AT awatalao introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT ifiebe introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT danquahe introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT jumbomb introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT sureshlm introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT gowdam introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT marchelodraggaphilipw introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT olsenms introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT shorinolaoluwaseyi introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT yaonasser introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT boddupallipm introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT tongoonapb introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya |