dsRNA molecules from the tobacco mosaic virus p126 gene counteract TMV-induced proteome changes at an early stage of infection
Exogenous application of double-stranded RNA (dsRNA) in the tobacco–Tobacco mosaic virus (TMV) pathosystem was shown previously to induce resistance against TMV providing an alternative approach to transgenesis. In the present study, we employed proteomics technology to elucidate the effect of TMV o...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Frontiers Media
2021
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/113883 |
| _version_ | 1855528550203916288 |
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| author | Konakalla, Naga Charan Nitin, Mukesh Kaldis, Athanasios Masarapu, Herma Carpentier, Sebastien C. Voloudakis, Andreas |
| author_browse | Carpentier, Sebastien C. Kaldis, Athanasios Konakalla, Naga Charan Masarapu, Herma Nitin, Mukesh Voloudakis, Andreas |
| author_facet | Konakalla, Naga Charan Nitin, Mukesh Kaldis, Athanasios Masarapu, Herma Carpentier, Sebastien C. Voloudakis, Andreas |
| author_sort | Konakalla, Naga Charan |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Exogenous application of double-stranded RNA (dsRNA) in the tobacco–Tobacco mosaic virus (TMV) pathosystem was shown previously to induce resistance against TMV providing an alternative approach to transgenesis. In the present study, we employed proteomics technology to elucidate the effect of TMV on tobacco as well as the effect of exogenous application of TMV p126 dsRNA molecules (dsRNAp126) at an early stage of the tobacco–TMV interaction. The proteome of tobacco leaf at 15 min post inoculation (mpi) in the presence or absence of dsRNAp126 molecules was studied. Thirty-six tobacco proteins were differentially accumulated in TMV-infected vs. healthy tobacco leaf tissue. The identified main differential TMV-responsive proteins were found to be involved in photosynthesis, energy metabolism, stress, and defense responses. Most of the virus-induced changes in the tobacco leaf proteome were not observed in the leaves treated with dsRNAp126 + TMV. The results indicated that the protein changes induced by TMV infection were counteracted by the exogenous application of dsRNAp126 molecules. Moreover, using small RNA sequencing, we showed that the exogenously applied dsRNAp126 was efficiently processed in tobacco as early as 15 min post application (mpa) to produce small interfering RNAs (siRNAs); the dicing pattern was not affected by the presence of TMV. The presence of dsRNAp126 reduced TMV p126 RNA abundance suggesting virus titer reduction via a sequence-specific mechanism, since a non-homologous dsRNA did not protect from TMV infection nor affect TMV accumulation. |
| format | Journal Article |
| id | CGSpace113883 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1138832025-11-11T18:52:19Z dsRNA molecules from the tobacco mosaic virus p126 gene counteract TMV-induced proteome changes at an early stage of infection Konakalla, Naga Charan Nitin, Mukesh Kaldis, Athanasios Masarapu, Herma Carpentier, Sebastien C. Voloudakis, Andreas proteomics rna tobacco mosaic tobamovirus plant defence reactions proteómica arn tobamovirus del mosaico del tabaco reacciones de defensa de plantas Exogenous application of double-stranded RNA (dsRNA) in the tobacco–Tobacco mosaic virus (TMV) pathosystem was shown previously to induce resistance against TMV providing an alternative approach to transgenesis. In the present study, we employed proteomics technology to elucidate the effect of TMV on tobacco as well as the effect of exogenous application of TMV p126 dsRNA molecules (dsRNAp126) at an early stage of the tobacco–TMV interaction. The proteome of tobacco leaf at 15 min post inoculation (mpi) in the presence or absence of dsRNAp126 molecules was studied. Thirty-six tobacco proteins were differentially accumulated in TMV-infected vs. healthy tobacco leaf tissue. The identified main differential TMV-responsive proteins were found to be involved in photosynthesis, energy metabolism, stress, and defense responses. Most of the virus-induced changes in the tobacco leaf proteome were not observed in the leaves treated with dsRNAp126 + TMV. The results indicated that the protein changes induced by TMV infection were counteracted by the exogenous application of dsRNAp126 molecules. Moreover, using small RNA sequencing, we showed that the exogenously applied dsRNAp126 was efficiently processed in tobacco as early as 15 min post application (mpa) to produce small interfering RNAs (siRNAs); the dicing pattern was not affected by the presence of TMV. The presence of dsRNAp126 reduced TMV p126 RNA abundance suggesting virus titer reduction via a sequence-specific mechanism, since a non-homologous dsRNA did not protect from TMV infection nor affect TMV accumulation. 2021-05 2021-06-08T07:01:29Z 2021-06-08T07:01:29Z Journal Article https://hdl.handle.net/10568/113883 en Open Access application/pdf Frontiers Media Konakalla, N.C.; Nitin, M.; Kaldis, A.; Masarapu, H.; Carpentier, S.; Voloudakis, A. (2021) dsRNA molecules from the tobacco mosaic virus p126 gene counteract TMV-induced proteome changes at an early stage of infection. Frontiers in Plant Science 12:663707. ISSN: 1664-462X |
| spellingShingle | proteomics rna tobacco mosaic tobamovirus plant defence reactions proteómica arn tobamovirus del mosaico del tabaco reacciones de defensa de plantas Konakalla, Naga Charan Nitin, Mukesh Kaldis, Athanasios Masarapu, Herma Carpentier, Sebastien C. Voloudakis, Andreas dsRNA molecules from the tobacco mosaic virus p126 gene counteract TMV-induced proteome changes at an early stage of infection |
| title | dsRNA molecules from the tobacco mosaic virus p126 gene counteract TMV-induced proteome changes at an early stage of infection |
| title_full | dsRNA molecules from the tobacco mosaic virus p126 gene counteract TMV-induced proteome changes at an early stage of infection |
| title_fullStr | dsRNA molecules from the tobacco mosaic virus p126 gene counteract TMV-induced proteome changes at an early stage of infection |
| title_full_unstemmed | dsRNA molecules from the tobacco mosaic virus p126 gene counteract TMV-induced proteome changes at an early stage of infection |
| title_short | dsRNA molecules from the tobacco mosaic virus p126 gene counteract TMV-induced proteome changes at an early stage of infection |
| title_sort | dsrna molecules from the tobacco mosaic virus p126 gene counteract tmv induced proteome changes at an early stage of infection |
| topic | proteomics rna tobacco mosaic tobamovirus plant defence reactions proteómica arn tobamovirus del mosaico del tabaco reacciones de defensa de plantas |
| url | https://hdl.handle.net/10568/113883 |
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