Genetic diversity and population structure of a rhodes grass (Chloris gayana) collection

Rhodes grass (Chloris gayana Kunth) is one of the most important forage grasses used throughout the tropical and subtropical regions of the world. Enhancing the conservation and use of genetic resources requires the development of knowledge and understanding about the existing global diversity of th...

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Autores principales: Negawo, Alemayehu T., Muktar, Meki S., Assefa, Yilikal, Hanson, Jean, Sartie, Alieu, Habte, Ermias, Jones, Christopher S.
Formato: Journal Article
Lenguaje:Inglés
Publicado: MDPI 2021
Materias:
Acceso en línea:https://hdl.handle.net/10568/113805
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author Negawo, Alemayehu T.
Muktar, Meki S.
Assefa, Yilikal
Hanson, Jean
Sartie, Alieu
Habte, Ermias
Jones, Christopher S.
author_browse Assefa, Yilikal
Habte, Ermias
Hanson, Jean
Jones, Christopher S.
Muktar, Meki S.
Negawo, Alemayehu T.
Sartie, Alieu
author_facet Negawo, Alemayehu T.
Muktar, Meki S.
Assefa, Yilikal
Hanson, Jean
Sartie, Alieu
Habte, Ermias
Jones, Christopher S.
author_sort Negawo, Alemayehu T.
collection Repository of Agricultural Research Outputs (CGSpace)
description Rhodes grass (Chloris gayana Kunth) is one of the most important forage grasses used throughout the tropical and subtropical regions of the world. Enhancing the conservation and use of genetic resources requires the development of knowledge and understanding about the existing global diversity of the species. In this study, 104 Rhodes grass accessions, held in trust in the ILRI forage genebank, were characterized using DArTSeq markers to evaluate the genetic diversity and population structure, and to develop representative subsets, of the collection. The genotyping produced 193,988 SNP and 142,522 SilicoDArT markers with an average polymorphic information content of 0.18 and 0.26, respectively. Hierarchical clustering using selected informative markers showed the presence of two and three main clusters using SNP and SilicoDArT markers, respectively, with a cophenetic correction coefficient of 82%. Bayesian population structure analysis also showed the presence of two main subpopulations using both marker types indicating the existence of significant genetic variation in the collection. A representative subset, containing 21 accessions from diverse origins, was developed using the SNP markers. In general, the results revealed substantial genetic diversity in the Rhodes grass collection, and the generated molecular information, together with the developed subset, should help enhance the management, use and improvement of Rhodes grass germplasm in the future.
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spelling CGSpace1138052025-02-19T11:00:29Z Genetic diversity and population structure of a rhodes grass (Chloris gayana) collection Negawo, Alemayehu T. Muktar, Meki S. Assefa, Yilikal Hanson, Jean Sartie, Alieu Habte, Ermias Jones, Christopher S. grasses forage animal feeding research feeds Rhodes grass (Chloris gayana Kunth) is one of the most important forage grasses used throughout the tropical and subtropical regions of the world. Enhancing the conservation and use of genetic resources requires the development of knowledge and understanding about the existing global diversity of the species. In this study, 104 Rhodes grass accessions, held in trust in the ILRI forage genebank, were characterized using DArTSeq markers to evaluate the genetic diversity and population structure, and to develop representative subsets, of the collection. The genotyping produced 193,988 SNP and 142,522 SilicoDArT markers with an average polymorphic information content of 0.18 and 0.26, respectively. Hierarchical clustering using selected informative markers showed the presence of two and three main clusters using SNP and SilicoDArT markers, respectively, with a cophenetic correction coefficient of 82%. Bayesian population structure analysis also showed the presence of two main subpopulations using both marker types indicating the existence of significant genetic variation in the collection. A representative subset, containing 21 accessions from diverse origins, was developed using the SNP markers. In general, the results revealed substantial genetic diversity in the Rhodes grass collection, and the generated molecular information, together with the developed subset, should help enhance the management, use and improvement of Rhodes grass germplasm in the future. 2021-08-10 2021-05-27T13:52:49Z 2021-05-27T13:52:49Z Journal Article https://hdl.handle.net/10568/113805 en Open Access MDPI Negawo A.T., Muktar, M.S., Assefa, Y., Hanson, J., Sartie, A.M., Habte, E. and Jones, C.S. 2021. Genetic diversity and population structure of a rhodes grass (Chloris gayana) collection. Genes 12(8):1233.
spellingShingle grasses
forage
animal feeding
research
feeds
Negawo, Alemayehu T.
Muktar, Meki S.
Assefa, Yilikal
Hanson, Jean
Sartie, Alieu
Habte, Ermias
Jones, Christopher S.
Genetic diversity and population structure of a rhodes grass (Chloris gayana) collection
title Genetic diversity and population structure of a rhodes grass (Chloris gayana) collection
title_full Genetic diversity and population structure of a rhodes grass (Chloris gayana) collection
title_fullStr Genetic diversity and population structure of a rhodes grass (Chloris gayana) collection
title_full_unstemmed Genetic diversity and population structure of a rhodes grass (Chloris gayana) collection
title_short Genetic diversity and population structure of a rhodes grass (Chloris gayana) collection
title_sort genetic diversity and population structure of a rhodes grass chloris gayana collection
topic grasses
forage
animal feeding
research
feeds
url https://hdl.handle.net/10568/113805
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