A comparison of differential gene expression in response to the onset of water stress between three hybrid Brachiaria genotypes
Brachiaria (Trin.) Griseb. (syn. Urochloa P. Beauv.) is a C4 grass genus belonging to the Panicoideae. Native to Africa, these grasses are now widely grown as forages in tropical areas worldwide and are the subject of intensive breeding, particularly in South America. Tolerance to abiotic stresses s...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Frontiers Media
2021
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/113731 |
| _version_ | 1855542420525023232 |
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| author | Jones, Charlotte Vega, José de Worthington, Margaret Thomas, Ann Gasior, Dagmara Harper, John Doonan, John Fu, Yuan Bosch, Maurice Corke, Fiona Arango, Jacobo Cardoso Arango, Juan Andrés Jiménez, Juan de la Cruz Armstead, Ian Fernandez-Fuentes, Narcis |
| author_browse | Arango, Jacobo Armstead, Ian Bosch, Maurice Cardoso Arango, Juan Andrés Corke, Fiona Doonan, John Fernandez-Fuentes, Narcis Fu, Yuan Gasior, Dagmara Harper, John Jiménez, Juan de la Cruz Jones, Charlotte Thomas, Ann Vega, José de Worthington, Margaret |
| author_facet | Jones, Charlotte Vega, José de Worthington, Margaret Thomas, Ann Gasior, Dagmara Harper, John Doonan, John Fu, Yuan Bosch, Maurice Corke, Fiona Arango, Jacobo Cardoso Arango, Juan Andrés Jiménez, Juan de la Cruz Armstead, Ian Fernandez-Fuentes, Narcis |
| author_sort | Jones, Charlotte |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Brachiaria (Trin.) Griseb. (syn. Urochloa P. Beauv.) is a C4 grass genus belonging to the Panicoideae. Native to Africa, these grasses are now widely grown as forages in tropical areas worldwide and are the subject of intensive breeding, particularly in South America. Tolerance to abiotic stresses such as aluminum and drought are major breeding objectives. In this study, we present the transcriptomic profiling of leaves and roots of three Brachiaria interspecific hybrid genotypes with the onset of water stress, Br12/3659-17 (gt-17), Br12/2360-9 (gt-9), and Br12/3868-18 (gt-18), previously characterized as having good, intermediate and poor tolerance to drought, respectively, in germplasm evaluation programs. RNA was extracted from leaf and root tissue of plants at estimated growing medium water contents (EWC) of 35, 15, and 5%. Differentially expressed genes (DEGs) were compared between different EWCs, 35/15, 15/5, and 35/5 using DESeq2. Overall, the proportions of DEGs enriched in all three genotypes varied in a genotype-dependent manner in relation to EWC comparison, with intermediate and sensitive gt-9 and gt-18 being more similar to each other than to drought tolerant gt-17. More specifically, GO terms relating to carbohydrate and cell wall metabolism in the leaves were enriched by up-regulated DEGs in gt-9 and gt-18, but by down-regulated DEGs in gt-17. Across all genotypes, analysis of DEG enzyme activities indicated an excess of down-regulated putative apoplastic peroxidases in the roots as water stress increased. This suggests that changes in root cell-wall architecture may be an important component of the response to water stress in Brachiaria. |
| format | Journal Article |
| id | CGSpace113731 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1137312025-11-11T19:09:06Z A comparison of differential gene expression in response to the onset of water stress between three hybrid Brachiaria genotypes Jones, Charlotte Vega, José de Worthington, Margaret Thomas, Ann Gasior, Dagmara Harper, John Doonan, John Fu, Yuan Bosch, Maurice Corke, Fiona Arango, Jacobo Cardoso Arango, Juan Andrés Jiménez, Juan de la Cruz Armstead, Ian Fernandez-Fuentes, Narcis abiotic stress cellars drought stress gene expression estrés abiótico bodegas estrés de sequia Brachiaria (Trin.) Griseb. (syn. Urochloa P. Beauv.) is a C4 grass genus belonging to the Panicoideae. Native to Africa, these grasses are now widely grown as forages in tropical areas worldwide and are the subject of intensive breeding, particularly in South America. Tolerance to abiotic stresses such as aluminum and drought are major breeding objectives. In this study, we present the transcriptomic profiling of leaves and roots of three Brachiaria interspecific hybrid genotypes with the onset of water stress, Br12/3659-17 (gt-17), Br12/2360-9 (gt-9), and Br12/3868-18 (gt-18), previously characterized as having good, intermediate and poor tolerance to drought, respectively, in germplasm evaluation programs. RNA was extracted from leaf and root tissue of plants at estimated growing medium water contents (EWC) of 35, 15, and 5%. Differentially expressed genes (DEGs) were compared between different EWCs, 35/15, 15/5, and 35/5 using DESeq2. Overall, the proportions of DEGs enriched in all three genotypes varied in a genotype-dependent manner in relation to EWC comparison, with intermediate and sensitive gt-9 and gt-18 being more similar to each other than to drought tolerant gt-17. More specifically, GO terms relating to carbohydrate and cell wall metabolism in the leaves were enriched by up-regulated DEGs in gt-9 and gt-18, but by down-regulated DEGs in gt-17. Across all genotypes, analysis of DEG enzyme activities indicated an excess of down-regulated putative apoplastic peroxidases in the roots as water stress increased. This suggests that changes in root cell-wall architecture may be an important component of the response to water stress in Brachiaria. 2021-03-19 2021-05-18T10:56:29Z 2021-05-18T10:56:29Z Journal Article https://hdl.handle.net/10568/113731 en Open Access application/pdf Frontiers Media Jones, C.; De Vega, J.; Worthington, M.; Thomas, A.; Gasior, D.; Harper, J.; Doonan, J.; Fu, Y.; Bosch, M.; Corke, F.; Arango, J.; Cardoso, J.A.; Jimenez, J.de la Cruz; Armstead, I.; Fernandez-Fuentes, N. (2021) A comparison of differential gene expression in response to the onset of water stress between three hybrid Brachiaria genotypes. Frontiers in Plant Science 12: 637956. 16 p. ISSN: 1664-462X |
| spellingShingle | abiotic stress cellars drought stress gene expression estrés abiótico bodegas estrés de sequia Jones, Charlotte Vega, José de Worthington, Margaret Thomas, Ann Gasior, Dagmara Harper, John Doonan, John Fu, Yuan Bosch, Maurice Corke, Fiona Arango, Jacobo Cardoso Arango, Juan Andrés Jiménez, Juan de la Cruz Armstead, Ian Fernandez-Fuentes, Narcis A comparison of differential gene expression in response to the onset of water stress between three hybrid Brachiaria genotypes |
| title | A comparison of differential gene expression in response to the onset of water stress between three hybrid Brachiaria genotypes |
| title_full | A comparison of differential gene expression in response to the onset of water stress between three hybrid Brachiaria genotypes |
| title_fullStr | A comparison of differential gene expression in response to the onset of water stress between three hybrid Brachiaria genotypes |
| title_full_unstemmed | A comparison of differential gene expression in response to the onset of water stress between three hybrid Brachiaria genotypes |
| title_short | A comparison of differential gene expression in response to the onset of water stress between three hybrid Brachiaria genotypes |
| title_sort | comparison of differential gene expression in response to the onset of water stress between three hybrid brachiaria genotypes |
| topic | abiotic stress cellars drought stress gene expression estrés abiótico bodegas estrés de sequia |
| url | https://hdl.handle.net/10568/113731 |
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