A compendium and comparative epigenomics analysis of cis-regulatory elements in the pig genome

Although major advances in genomics have initiated an exciting new era of research, a lack of information regarding cis-regulatory elements has limited the genetic improvement or manipulation of pigs as a meat source and biomedical model. Here, we systematically characterize cis-regulatory elements...

Full description

Bibliographic Details
Main Authors: Yunxia Zhao, Ye Hou, Yueyuan Xu, Yu Luan, Huanhuan Zhou, Xiaolong Qi, Mingyang Hu, Daoyuan Wang, Zhangxu Wang, Yuhua Fu, Jingjin Li, Saixian Zhang, Jianhai Chen, Han Jianlin, Xinyun Li, Shuhong Zhao
Format: Journal Article
Language:Inglés
Published: Springer 2021
Subjects:
Online Access:https://hdl.handle.net/10568/113497
_version_ 1855525079094394880
author Yunxia Zhao
Ye Hou
Yueyuan Xu
Yu Luan
Huanhuan Zhou
Xiaolong Qi
Mingyang Hu
Daoyuan Wang
Zhangxu Wang
Yuhua Fu
Jingjin Li
Saixian Zhang
Jianhai Chen
Han Jianlin
Xinyun Li
Shuhong Zhao
author_browse Daoyuan Wang
Han Jianlin
Huanhuan Zhou
Jianhai Chen
Jingjin Li
Mingyang Hu
Saixian Zhang
Shuhong Zhao
Xiaolong Qi
Xinyun Li
Ye Hou
Yu Luan
Yueyuan Xu
Yuhua Fu
Yunxia Zhao
Zhangxu Wang
author_facet Yunxia Zhao
Ye Hou
Yueyuan Xu
Yu Luan
Huanhuan Zhou
Xiaolong Qi
Mingyang Hu
Daoyuan Wang
Zhangxu Wang
Yuhua Fu
Jingjin Li
Saixian Zhang
Jianhai Chen
Han Jianlin
Xinyun Li
Shuhong Zhao
author_sort Yunxia Zhao
collection Repository of Agricultural Research Outputs (CGSpace)
description Although major advances in genomics have initiated an exciting new era of research, a lack of information regarding cis-regulatory elements has limited the genetic improvement or manipulation of pigs as a meat source and biomedical model. Here, we systematically characterize cis-regulatory elements and their functions in 12 diverse tissues from four pig breeds by adopting similar strategies as the ENCODE and Roadmap Epigenomics projects, which include RNA-seq, ATAC-seq, and ChIP-seq. In total, we generate 199 datasets and identify more than 220,000 cis-regulatory elements in the pig genome. Surprisingly, we find higher conservation of cis-regulatory elements between human and pig genomes than those between human and mouse genomes. Furthermore, the differences of topologically associating domains between the pig and human genomes are associated with morphological evolution of the head and face. Beyond generating a major new benchmark resource for pig epigenetics, our study provides basic comparative epigenetic data relevant to using pigs as models in human biomedical research.
format Journal Article
id CGSpace113497
institution CGIAR Consortium
language Inglés
publishDate 2021
publishDateRange 2021
publishDateSort 2021
publisher Springer
publisherStr Springer
record_format dspace
spelling CGSpace1134972024-05-01T08:17:20Z A compendium and comparative epigenomics analysis of cis-regulatory elements in the pig genome Yunxia Zhao Ye Hou Yueyuan Xu Yu Luan Huanhuan Zhou Xiaolong Qi Mingyang Hu Daoyuan Wang Zhangxu Wang Yuhua Fu Jingjin Li Saixian Zhang Jianhai Chen Han Jianlin Xinyun Li Shuhong Zhao swine genetics genomes animal breeding Although major advances in genomics have initiated an exciting new era of research, a lack of information regarding cis-regulatory elements has limited the genetic improvement or manipulation of pigs as a meat source and biomedical model. Here, we systematically characterize cis-regulatory elements and their functions in 12 diverse tissues from four pig breeds by adopting similar strategies as the ENCODE and Roadmap Epigenomics projects, which include RNA-seq, ATAC-seq, and ChIP-seq. In total, we generate 199 datasets and identify more than 220,000 cis-regulatory elements in the pig genome. Surprisingly, we find higher conservation of cis-regulatory elements between human and pig genomes than those between human and mouse genomes. Furthermore, the differences of topologically associating domains between the pig and human genomes are associated with morphological evolution of the head and face. Beyond generating a major new benchmark resource for pig epigenetics, our study provides basic comparative epigenetic data relevant to using pigs as models in human biomedical research. 2021-04-13 2021-04-22T19:11:05Z 2021-04-22T19:11:05Z Journal Article https://hdl.handle.net/10568/113497 en Open Access Springer Yunxia Zhao, Ye Hou, Yueyuan Xu, Yu Luan, Huanhuan Zhou, Xiaolong Qi, Mingyang Hu, Daoyuan Wang, Zhangxu Wang, Yuhua Fu, Jingjin Li, Saixian Zhang, Jianhai Chen, Jianlin Han, Xinyun Li and Shuhong Zhao. 2021. A compendium and comparative epigenomics analysis of cis-regulatory elements in the pig genome. Nature Communications 12:2217
spellingShingle swine
genetics
genomes
animal breeding
Yunxia Zhao
Ye Hou
Yueyuan Xu
Yu Luan
Huanhuan Zhou
Xiaolong Qi
Mingyang Hu
Daoyuan Wang
Zhangxu Wang
Yuhua Fu
Jingjin Li
Saixian Zhang
Jianhai Chen
Han Jianlin
Xinyun Li
Shuhong Zhao
A compendium and comparative epigenomics analysis of cis-regulatory elements in the pig genome
title A compendium and comparative epigenomics analysis of cis-regulatory elements in the pig genome
title_full A compendium and comparative epigenomics analysis of cis-regulatory elements in the pig genome
title_fullStr A compendium and comparative epigenomics analysis of cis-regulatory elements in the pig genome
title_full_unstemmed A compendium and comparative epigenomics analysis of cis-regulatory elements in the pig genome
title_short A compendium and comparative epigenomics analysis of cis-regulatory elements in the pig genome
title_sort compendium and comparative epigenomics analysis of cis regulatory elements in the pig genome
topic swine
genetics
genomes
animal breeding
url https://hdl.handle.net/10568/113497
work_keys_str_mv AT yunxiazhao acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT yehou acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT yueyuanxu acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT yuluan acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT huanhuanzhou acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT xiaolongqi acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT mingyanghu acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT daoyuanwang acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT zhangxuwang acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT yuhuafu acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT jingjinli acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT saixianzhang acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT jianhaichen acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT hanjianlin acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT xinyunli acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT shuhongzhao acompendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT yunxiazhao compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT yehou compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT yueyuanxu compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT yuluan compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT huanhuanzhou compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT xiaolongqi compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT mingyanghu compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT daoyuanwang compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT zhangxuwang compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT yuhuafu compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT jingjinli compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT saixianzhang compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT jianhaichen compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT hanjianlin compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT xinyunli compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome
AT shuhongzhao compendiumandcomparativeepigenomicsanalysisofcisregulatoryelementsinthepiggenome