Auxin signaling and vascular cambium formation enables storage metabolism in cassava tuberous roots

Cassava storage roots are among the most important root crops worldwide and represent one of the most consumed staple foods in Sub-Saharan Africa. The vegetatively propagated tropical shrub can form many starchy tuberous roots from its stem. These storage roots are formed through the activation of s...

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Main Authors: Rüscher, D., García, J.M.C., Carluccio, A.V., Klemens, P.A.W., Gisel, A., Stavolone, L., Neuhaus, H.E., Ludewig, F., Sonnewald, U., Zierer, W.
Format: Journal Article
Language:Inglés
Published: Oxford University Press 2021
Subjects:
Online Access:https://hdl.handle.net/10568/113490
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author Rüscher, D.
García, J.M.C.
Carluccio, A.V.
Klemens, P.A.W.
Gisel, A.
Stavolone, L.
Neuhaus, H.E.
Ludewig, F.
Sonnewald, U.
Zierer, W.
author_browse Carluccio, A.V.
García, J.M.C.
Gisel, A.
Klemens, P.A.W.
Ludewig, F.
Neuhaus, H.E.
Rüscher, D.
Sonnewald, U.
Stavolone, L.
Zierer, W.
author_facet Rüscher, D.
García, J.M.C.
Carluccio, A.V.
Klemens, P.A.W.
Gisel, A.
Stavolone, L.
Neuhaus, H.E.
Ludewig, F.
Sonnewald, U.
Zierer, W.
author_sort Rüscher, D.
collection Repository of Agricultural Research Outputs (CGSpace)
description Cassava storage roots are among the most important root crops worldwide and represent one of the most consumed staple foods in Sub-Saharan Africa. The vegetatively propagated tropical shrub can form many starchy tuberous roots from its stem. These storage roots are formed through the activation of secondary root growth processes. However, the underlying genetic regulation of storage root development is largely unknown. Here we report on distinct structural and transcriptional changes occurring during the early phases of storage root development. A pronounced increase in auxin-related transcripts and the transcriptional activation of secondary growth factors, as well as a decrease in gibberellin-related transcripts was observed during the early stages of secondary root growth. This was accompanied by increased cell wall biosynthesis, increased most notably during the initial xylem expansion within the root vasculature. Starch storage metabolism was activated only after the formation of the vascular cambium. The formation of nonlignified xylem parenchyma cells and the activation of starch storage metabolism coincided with increased expression of the KNOX/BEL genes KNAT1, PENNYWISE and POUND-FOOLISH, indicating their importance for proper xylem parenchyma function.
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spelling CGSpace1134902025-12-08T09:54:28Z Auxin signaling and vascular cambium formation enables storage metabolism in cassava tuberous roots Rüscher, D. García, J.M.C. Carluccio, A.V. Klemens, P.A.W. Gisel, A. Stavolone, L. Neuhaus, H.E. Ludewig, F. Sonnewald, U. Zierer, W. cassava auxins development gibberellins parenchyma roots starch storage xylem Cassava storage roots are among the most important root crops worldwide and represent one of the most consumed staple foods in Sub-Saharan Africa. The vegetatively propagated tropical shrub can form many starchy tuberous roots from its stem. These storage roots are formed through the activation of secondary root growth processes. However, the underlying genetic regulation of storage root development is largely unknown. Here we report on distinct structural and transcriptional changes occurring during the early phases of storage root development. A pronounced increase in auxin-related transcripts and the transcriptional activation of secondary growth factors, as well as a decrease in gibberellin-related transcripts was observed during the early stages of secondary root growth. This was accompanied by increased cell wall biosynthesis, increased most notably during the initial xylem expansion within the root vasculature. Starch storage metabolism was activated only after the formation of the vascular cambium. The formation of nonlignified xylem parenchyma cells and the activation of starch storage metabolism coincided with increased expression of the KNOX/BEL genes KNAT1, PENNYWISE and POUND-FOOLISH, indicating their importance for proper xylem parenchyma function. 2021-03-13 2021-04-22T10:11:19Z 2021-04-22T10:11:19Z Journal Article https://hdl.handle.net/10568/113490 en Open Access application/pdf Oxford University Press Rüscher, D., García, J.M.C., Carluccio, A.V., Klemens, P.A., Gisel, A., Stavolone, L., ... & Zierer, W. (2021). Auxin signaling and vascular cambium formation enables storage metabolism in cassava tuberous roots. Journal of Experimental Botany, 1-33.
spellingShingle cassava
auxins
development
gibberellins
parenchyma
roots
starch
storage
xylem
Rüscher, D.
García, J.M.C.
Carluccio, A.V.
Klemens, P.A.W.
Gisel, A.
Stavolone, L.
Neuhaus, H.E.
Ludewig, F.
Sonnewald, U.
Zierer, W.
Auxin signaling and vascular cambium formation enables storage metabolism in cassava tuberous roots
title Auxin signaling and vascular cambium formation enables storage metabolism in cassava tuberous roots
title_full Auxin signaling and vascular cambium formation enables storage metabolism in cassava tuberous roots
title_fullStr Auxin signaling and vascular cambium formation enables storage metabolism in cassava tuberous roots
title_full_unstemmed Auxin signaling and vascular cambium formation enables storage metabolism in cassava tuberous roots
title_short Auxin signaling and vascular cambium formation enables storage metabolism in cassava tuberous roots
title_sort auxin signaling and vascular cambium formation enables storage metabolism in cassava tuberous roots
topic cassava
auxins
development
gibberellins
parenchyma
roots
starch
storage
xylem
url https://hdl.handle.net/10568/113490
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