Giant milkweed (Calotropis gigantea): A new plant resource to inhibit protozoa and decrease ammoniagenesis of rumen microbiota in vitro without impairing fermentation
This study screened six different species of forest plants and then further evaluated the most promising plant, giant milkweed (Calotropis gigantea), for the potential to improve nitrogen utilization efficiency (NUE) through inhibiting rumen protozoa in vitro. Ground leaves of giant milkweed at 1.6...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/113353 |
| _version_ | 1855520196852187136 |
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| author | Ayemele, A.G. Ma, L. Park, T. Xu, J.C. Yu, Z.T. Bu, D.P. |
| author_browse | Ayemele, A.G. Bu, D.P. Ma, L. Park, T. Xu, J.C. Yu, Z.T. |
| author_facet | Ayemele, A.G. Ma, L. Park, T. Xu, J.C. Yu, Z.T. Bu, D.P. |
| author_sort | Ayemele, A.G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | This study screened six different species of forest plants and then further evaluated the most promising plant, giant milkweed (Calotropis gigantea), for the potential to improve nitrogen utilization efficiency (NUE) through inhibiting rumen protozoa in vitro. Ground leaves of giant milkweed at 1.6 and 3.2 mg/mL decreased the counts of Entodinium cells by 41.30% and 58.89%, respectively, and damaged their cell surface structure. Dasytricha, Isotricha, Epidinium, Ophryoscolex, and Diplodinium were not affected, while total bacterial and archaeal populations did not decrease. Ammonia nitrogen (NH3-N) concentration decreased by 50.64% and 33.33% at 1.6 g/mL and 3.2 mg/mL, respectively. Volatile fatty acid (VFA) production and methane production remained unaffected, but butyrate production increased. The giant milkweed leaves contained (per gram of dry matter) 3636 μg phenolics including 205.9 μg of 3-hydroxybenzoic acid, 2079 μg flavonoids including 1197.5 μg of quercetin and 91.4 μg of myricetin, and 490 μg alkaloids including 219.8 μg of anthraquinone glycosides. The effective inhibition of Entodinium was accompanied by a decrease in NH3-N concentration, and methane production did not increase except for the dose of 1.6 mg/mL. Giant milkweed may be used as a new feed additive or an alternative to chemicals or antibiotics for sustainable animal husbandry enhancing NUE in ruminants. |
| format | Journal Article |
| id | CGSpace113353 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1133532025-01-24T14:12:28Z Giant milkweed (Calotropis gigantea): A new plant resource to inhibit protozoa and decrease ammoniagenesis of rumen microbiota in vitro without impairing fermentation Ayemele, A.G. Ma, L. Park, T. Xu, J.C. Yu, Z.T. Bu, D.P. medicinal plants methane production ammonium nitrogen pollution environmental engineering This study screened six different species of forest plants and then further evaluated the most promising plant, giant milkweed (Calotropis gigantea), for the potential to improve nitrogen utilization efficiency (NUE) through inhibiting rumen protozoa in vitro. Ground leaves of giant milkweed at 1.6 and 3.2 mg/mL decreased the counts of Entodinium cells by 41.30% and 58.89%, respectively, and damaged their cell surface structure. Dasytricha, Isotricha, Epidinium, Ophryoscolex, and Diplodinium were not affected, while total bacterial and archaeal populations did not decrease. Ammonia nitrogen (NH3-N) concentration decreased by 50.64% and 33.33% at 1.6 g/mL and 3.2 mg/mL, respectively. Volatile fatty acid (VFA) production and methane production remained unaffected, but butyrate production increased. The giant milkweed leaves contained (per gram of dry matter) 3636 μg phenolics including 205.9 μg of 3-hydroxybenzoic acid, 2079 μg flavonoids including 1197.5 μg of quercetin and 91.4 μg of myricetin, and 490 μg alkaloids including 219.8 μg of anthraquinone glycosides. The effective inhibition of Entodinium was accompanied by a decrease in NH3-N concentration, and methane production did not increase except for the dose of 1.6 mg/mL. Giant milkweed may be used as a new feed additive or an alternative to chemicals or antibiotics for sustainable animal husbandry enhancing NUE in ruminants. 2020-11 2021-04-15T03:35:42Z 2021-04-15T03:35:42Z Journal Article https://hdl.handle.net/10568/113353 en Limited Access Elsevier Ayemele, A.G., Ma, L., Park, T., Xu, J.C., Yu, Z.T., Bu, D.P., 2020. Giant milkweed (Calotropis gigantea): A new plant resource to inhibit protozoa and decrease ammoniagenesis of rumen microbiota in vitro without impairing fermentation. Science of The Total Environment, 743: 140665. https://doi.org/10.1016/j.scitotenv.2020.140665 |
| spellingShingle | medicinal plants methane production ammonium nitrogen pollution environmental engineering Ayemele, A.G. Ma, L. Park, T. Xu, J.C. Yu, Z.T. Bu, D.P. Giant milkweed (Calotropis gigantea): A new plant resource to inhibit protozoa and decrease ammoniagenesis of rumen microbiota in vitro without impairing fermentation |
| title | Giant milkweed (Calotropis gigantea): A new plant resource to inhibit protozoa and decrease ammoniagenesis of rumen microbiota in vitro without impairing fermentation |
| title_full | Giant milkweed (Calotropis gigantea): A new plant resource to inhibit protozoa and decrease ammoniagenesis of rumen microbiota in vitro without impairing fermentation |
| title_fullStr | Giant milkweed (Calotropis gigantea): A new plant resource to inhibit protozoa and decrease ammoniagenesis of rumen microbiota in vitro without impairing fermentation |
| title_full_unstemmed | Giant milkweed (Calotropis gigantea): A new plant resource to inhibit protozoa and decrease ammoniagenesis of rumen microbiota in vitro without impairing fermentation |
| title_short | Giant milkweed (Calotropis gigantea): A new plant resource to inhibit protozoa and decrease ammoniagenesis of rumen microbiota in vitro without impairing fermentation |
| title_sort | giant milkweed calotropis gigantea a new plant resource to inhibit protozoa and decrease ammoniagenesis of rumen microbiota in vitro without impairing fermentation |
| topic | medicinal plants methane production ammonium nitrogen pollution environmental engineering |
| url | https://hdl.handle.net/10568/113353 |
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