Methicillin-resistant and methicillin-susceptible Staphylococcus from vervet monkeys (Chlorocebus sabaeus) in Saint Kitts
Antimicrobial resistance has been described in all ecosystems, including wildlife. Here we investigated the presence of methicillin-resistant and susceptible staphylococci in both colony-born and wild vervet monkeys (Chlorocebus sabaeus). Through selective isolation, PCR, MALDI-TOF, and whole-genome...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
MDPI
2021
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/113205 |
| _version_ | 1855528329716695040 |
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| author | Hoefer, A. Boyen, F. Beierschmitt, A. Moodley, Arshnee Roberts, M.C. Butaye, P. |
| author_browse | Beierschmitt, A. Boyen, F. Butaye, P. Hoefer, A. Moodley, Arshnee Roberts, M.C. |
| author_facet | Hoefer, A. Boyen, F. Beierschmitt, A. Moodley, Arshnee Roberts, M.C. Butaye, P. |
| author_sort | Hoefer, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Antimicrobial resistance has been described in all ecosystems, including wildlife. Here we investigated the presence of methicillin-resistant and susceptible staphylococci in both colony-born and wild vervet monkeys (Chlorocebus sabaeus). Through selective isolation, PCR, MALDI-TOF, and whole-genome sequencing, methicillin-resistant and susceptible Staphylococcus spp. isolated from vervet monkeys were characterized. We obtained putatively methicillin-resistant staphylococci from 29 of the 34 nasal samples collected. Strains were identified by MALDI-TOF analysis. Staphylococcus cohnii (n = 15) was the most commonly isolated species, while nine other species were isolated one or two times. PCR analysis indicated that eight [28%] strains were mecA positive. The whole-genome sequencing [WGS] included eight methicillin-resistant strains (S. epidermidis (n = 2), S. cohnii (n = 3), S. arlettae (n = 2) and S. hominis (n = 1)), nine additional S. cohnii strains and two strains that could not be identified by MALDI-TOF, but genetically characterized as one S. cohnii and one S. warneri. Different resistance genes carried by different mobile genetic elements, mainly blaZ (n = 10) and tet(K) (n = 5) were found, while msr(A), cat, fosB, dfrG, erm(C), mph(C) and str were identified in one to three strains. Phylogenetic analysis of the S. cohnii strains based on SNPs indicated four clusters associated with colony born or wild. In addition, one singleton S. cohnii isolated did not form a separate group and clustered within other S. cohnii strains submitted to the NCBI. In this study, we demonstrated the presence of AMR and mobile genetic elements to both colony-born and wild vervet monkeys. We also identified a previously undescribed prevalence of S. cohnii in the nasal flora of these monkeys, which merits further investigation. |
| format | Journal Article |
| id | CGSpace113205 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1132052025-08-15T13:22:07Z Methicillin-resistant and methicillin-susceptible Staphylococcus from vervet monkeys (Chlorocebus sabaeus) in Saint Kitts Hoefer, A. Boyen, F. Beierschmitt, A. Moodley, Arshnee Roberts, M.C. Butaye, P. antimicrobial resistance monkeys Antimicrobial resistance has been described in all ecosystems, including wildlife. Here we investigated the presence of methicillin-resistant and susceptible staphylococci in both colony-born and wild vervet monkeys (Chlorocebus sabaeus). Through selective isolation, PCR, MALDI-TOF, and whole-genome sequencing, methicillin-resistant and susceptible Staphylococcus spp. isolated from vervet monkeys were characterized. We obtained putatively methicillin-resistant staphylococci from 29 of the 34 nasal samples collected. Strains were identified by MALDI-TOF analysis. Staphylococcus cohnii (n = 15) was the most commonly isolated species, while nine other species were isolated one or two times. PCR analysis indicated that eight [28%] strains were mecA positive. The whole-genome sequencing [WGS] included eight methicillin-resistant strains (S. epidermidis (n = 2), S. cohnii (n = 3), S. arlettae (n = 2) and S. hominis (n = 1)), nine additional S. cohnii strains and two strains that could not be identified by MALDI-TOF, but genetically characterized as one S. cohnii and one S. warneri. Different resistance genes carried by different mobile genetic elements, mainly blaZ (n = 10) and tet(K) (n = 5) were found, while msr(A), cat, fosB, dfrG, erm(C), mph(C) and str were identified in one to three strains. Phylogenetic analysis of the S. cohnii strains based on SNPs indicated four clusters associated with colony born or wild. In addition, one singleton S. cohnii isolated did not form a separate group and clustered within other S. cohnii strains submitted to the NCBI. In this study, we demonstrated the presence of AMR and mobile genetic elements to both colony-born and wild vervet monkeys. We also identified a previously undescribed prevalence of S. cohnii in the nasal flora of these monkeys, which merits further investigation. 2021-03-10 2021-04-04T20:21:25Z 2021-04-04T20:21:25Z Journal Article https://hdl.handle.net/10568/113205 en Open Access MDPI Hoefer, A., Boyen, F., Beierschmitt, A., Moodley, A., Roberts, M.C. and Butaye, P. 2021. Methicillin-resistant and methicillin-susceptible Staphylococcus from vervet monkeys (Chlorocebus sabaeus) in Saint Kitts. Antibiotics 10(3): 290. |
| spellingShingle | antimicrobial resistance monkeys Hoefer, A. Boyen, F. Beierschmitt, A. Moodley, Arshnee Roberts, M.C. Butaye, P. Methicillin-resistant and methicillin-susceptible Staphylococcus from vervet monkeys (Chlorocebus sabaeus) in Saint Kitts |
| title | Methicillin-resistant and methicillin-susceptible Staphylococcus from vervet monkeys (Chlorocebus sabaeus) in Saint Kitts |
| title_full | Methicillin-resistant and methicillin-susceptible Staphylococcus from vervet monkeys (Chlorocebus sabaeus) in Saint Kitts |
| title_fullStr | Methicillin-resistant and methicillin-susceptible Staphylococcus from vervet monkeys (Chlorocebus sabaeus) in Saint Kitts |
| title_full_unstemmed | Methicillin-resistant and methicillin-susceptible Staphylococcus from vervet monkeys (Chlorocebus sabaeus) in Saint Kitts |
| title_short | Methicillin-resistant and methicillin-susceptible Staphylococcus from vervet monkeys (Chlorocebus sabaeus) in Saint Kitts |
| title_sort | methicillin resistant and methicillin susceptible staphylococcus from vervet monkeys chlorocebus sabaeus in saint kitts |
| topic | antimicrobial resistance monkeys |
| url | https://hdl.handle.net/10568/113205 |
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