A simple PCR-based method for the rapid and accurate identification of spider mites (Tetranychidae) on cassava
The morphological identification of mites entails great challenges. Characteristics such as dorsal setae and aedeagus are widely used, but they show variations between populations, and the technique is time consuming and demands specialized taxonomic expertise that is difficult to access. A successf...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/112939 |
| _version_ | 1855530562433843200 |
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| author | Ovalle Rivera, Tatiana M. Vásquez Ordoñez, Aymer Andrés Jiménez Polo, Jenyfer Parsa, Soroush Cuéllar, Wilmer Jose Becerra López Lavelle, Luis Augusto |
| author_browse | Becerra López Lavelle, Luis Augusto Cuéllar, Wilmer Jose Jiménez Polo, Jenyfer Ovalle Rivera, Tatiana M. Parsa, Soroush Vásquez Ordoñez, Aymer Andrés |
| author_facet | Ovalle Rivera, Tatiana M. Vásquez Ordoñez, Aymer Andrés Jiménez Polo, Jenyfer Parsa, Soroush Cuéllar, Wilmer Jose Becerra López Lavelle, Luis Augusto |
| author_sort | Ovalle Rivera, Tatiana M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The morphological identification of mites entails great challenges. Characteristics such as dorsal setae and aedeagus are widely used, but they show variations between populations, and the technique is time consuming and demands specialized taxonomic expertise that is difficult to access. A successful alternative has been to exploit a region of the mitochondrial cytochrome oxidase I (COI) gene to classify specimens to the species level. We analyzed the COI sequences of four mite species associated with cassava and classified them definitively by detailed morphological examinations. We then developed an identification kit based on the restriction fragment length polymorphism–polymerase chain reaction of subunit I of the COI gene focused on the three restriction enzymes AseI, MboII, and ApoI. This set of enzymes permitted the simple, accurate identification of Mononychellus caribbeanae, M. tanajoa, M. mcgregori, and Tetranychus urticae, rapidly and with few resources. This kit could be a vital tool for the surveillance and monitoring of mite pests in cassava crop protection programs in Africa, Asia, and Latin America. |
| format | Journal Article |
| id | CGSpace112939 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1129392023-12-27T19:43:38Z A simple PCR-based method for the rapid and accurate identification of spider mites (Tetranychidae) on cassava Ovalle Rivera, Tatiana M. Vásquez Ordoñez, Aymer Andrés Jiménez Polo, Jenyfer Parsa, Soroush Cuéllar, Wilmer Jose Becerra López Lavelle, Luis Augusto cassava tetranychidae The morphological identification of mites entails great challenges. Characteristics such as dorsal setae and aedeagus are widely used, but they show variations between populations, and the technique is time consuming and demands specialized taxonomic expertise that is difficult to access. A successful alternative has been to exploit a region of the mitochondrial cytochrome oxidase I (COI) gene to classify specimens to the species level. We analyzed the COI sequences of four mite species associated with cassava and classified them definitively by detailed morphological examinations. We then developed an identification kit based on the restriction fragment length polymorphism–polymerase chain reaction of subunit I of the COI gene focused on the three restriction enzymes AseI, MboII, and ApoI. This set of enzymes permitted the simple, accurate identification of Mononychellus caribbeanae, M. tanajoa, M. mcgregori, and Tetranychus urticae, rapidly and with few resources. This kit could be a vital tool for the surveillance and monitoring of mite pests in cassava crop protection programs in Africa, Asia, and Latin America. 2020-11-11 2021-03-11T01:33:42Z 2021-03-11T01:33:42Z Journal Article https://hdl.handle.net/10568/112939 en Open Access Springer Ovalle, T. M., Vásquez-Ordóñez, A. A., Jimenez, J., Parsa, S., Cuellar, W. J., & Becerra Lopez-Lavalle, L. A. (2020). A simple Pcr-based method for the rapid and accurate identification of spider mites (TETRANYCHIDAE) on cassava. Scientific Reports. ISSN: 2045-2322, 10(1). |
| spellingShingle | cassava tetranychidae Ovalle Rivera, Tatiana M. Vásquez Ordoñez, Aymer Andrés Jiménez Polo, Jenyfer Parsa, Soroush Cuéllar, Wilmer Jose Becerra López Lavelle, Luis Augusto A simple PCR-based method for the rapid and accurate identification of spider mites (Tetranychidae) on cassava |
| title | A simple PCR-based method for the rapid and accurate identification of spider mites (Tetranychidae) on cassava |
| title_full | A simple PCR-based method for the rapid and accurate identification of spider mites (Tetranychidae) on cassava |
| title_fullStr | A simple PCR-based method for the rapid and accurate identification of spider mites (Tetranychidae) on cassava |
| title_full_unstemmed | A simple PCR-based method for the rapid and accurate identification of spider mites (Tetranychidae) on cassava |
| title_short | A simple PCR-based method for the rapid and accurate identification of spider mites (Tetranychidae) on cassava |
| title_sort | simple pcr based method for the rapid and accurate identification of spider mites tetranychidae on cassava |
| topic | cassava tetranychidae |
| url | https://hdl.handle.net/10568/112939 |
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