An optimized high-quality DNA isolation protocol for spodoptera frugiperda J. E. smith (Lepidoptera: Noctuidae)

An optimized high-quality DNA isolation protocol was developed using body segment tissue from the Fall Armyworm (Spodoptera frugiperda), that will allow documenting genetic variability based on biotypes, facilitating studies on the appearance, distribution and population dynamics of the fall armywor...

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Main Authors: Marín L., Diana Victoria, Castillo, Diana Katherine, López Lavalle, Luis Augusto Becerra, Rodríguez Chalarca, Jairo, Pérez, Cristo Rafael
Format: Journal Article
Language:Inglés
Published: Elsevier 2021
Subjects:
Online Access:https://hdl.handle.net/10568/111747
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author Marín L., Diana Victoria
Castillo, Diana Katherine
López Lavalle, Luis Augusto Becerra
Rodríguez Chalarca, Jairo
Pérez, Cristo Rafael
author_browse Castillo, Diana Katherine
López Lavalle, Luis Augusto Becerra
Marín L., Diana Victoria
Pérez, Cristo Rafael
Rodríguez Chalarca, Jairo
author_facet Marín L., Diana Victoria
Castillo, Diana Katherine
López Lavalle, Luis Augusto Becerra
Rodríguez Chalarca, Jairo
Pérez, Cristo Rafael
author_sort Marín L., Diana Victoria
collection Repository of Agricultural Research Outputs (CGSpace)
description An optimized high-quality DNA isolation protocol was developed using body segment tissue from the Fall Armyworm (Spodoptera frugiperda), that will allow documenting genetic variability based on biotypes, facilitating studies on the appearance, distribution and population dynamics of the fall armyworm at the molecular level. The resulting protocol is an easy-to-use, timesaving method that can rapidly achieve high quality, high-yielding total genomic DNA, using chemicals and everyday consumables available in a molecular laboratory. This new method of DNA extraction avoids the contamination of polysaccharides, salts, phenols, proteins and other cellular by-products that can interfere with subsequent reactions. DNA purity estimates reveal A260: A280 ratios greater than 1.9, which were evidenced by quality test on agarose gel, observing complete integrity and high purity of the resulting samples, and yielded 30–99 µg/g of total DNA. Therefore, the quality of the DNA produced from this extraction is suitable for subsequent molecular applications: (i) next generation whole genome sequencing, (ii) conventional polymerase chain reaction for genotyping, (iii) barcodes and (iv) gene cloning.
format Journal Article
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spelling CGSpace1117472025-11-11T18:49:44Z An optimized high-quality DNA isolation protocol for spodoptera frugiperda J. E. smith (Lepidoptera: Noctuidae) Marín L., Diana Victoria Castillo, Diana Katherine López Lavalle, Luis Augusto Becerra Rodríguez Chalarca, Jairo Pérez, Cristo Rafael spodoptera frugiperda pests of plants dna identification methods isolation techniques plagas de plantas identificación An optimized high-quality DNA isolation protocol was developed using body segment tissue from the Fall Armyworm (Spodoptera frugiperda), that will allow documenting genetic variability based on biotypes, facilitating studies on the appearance, distribution and population dynamics of the fall armyworm at the molecular level. The resulting protocol is an easy-to-use, timesaving method that can rapidly achieve high quality, high-yielding total genomic DNA, using chemicals and everyday consumables available in a molecular laboratory. This new method of DNA extraction avoids the contamination of polysaccharides, salts, phenols, proteins and other cellular by-products that can interfere with subsequent reactions. DNA purity estimates reveal A260: A280 ratios greater than 1.9, which were evidenced by quality test on agarose gel, observing complete integrity and high purity of the resulting samples, and yielded 30–99 µg/g of total DNA. Therefore, the quality of the DNA produced from this extraction is suitable for subsequent molecular applications: (i) next generation whole genome sequencing, (ii) conventional polymerase chain reaction for genotyping, (iii) barcodes and (iv) gene cloning. 2021 2021-03-03T12:08:20Z 2021-03-03T12:08:20Z Journal Article https://hdl.handle.net/10568/111747 en Open Access application/pdf Elsevier Marín, D.V.; Castillo, D.K.; López-Lavalle, L.A.B.; Rodríguez Chalarca, J.; Pérez, C.R. (2021) An optimized high-quality DNA isolation protocol for spodoptera frugiperda J. E. smith (Lepidoptera: Noctuidae). MethodsX 8: 101255. ISSN: 2215-0161.
spellingShingle spodoptera frugiperda
pests of plants
dna
identification
methods
isolation techniques
plagas de plantas
identificación
Marín L., Diana Victoria
Castillo, Diana Katherine
López Lavalle, Luis Augusto Becerra
Rodríguez Chalarca, Jairo
Pérez, Cristo Rafael
An optimized high-quality DNA isolation protocol for spodoptera frugiperda J. E. smith (Lepidoptera: Noctuidae)
title An optimized high-quality DNA isolation protocol for spodoptera frugiperda J. E. smith (Lepidoptera: Noctuidae)
title_full An optimized high-quality DNA isolation protocol for spodoptera frugiperda J. E. smith (Lepidoptera: Noctuidae)
title_fullStr An optimized high-quality DNA isolation protocol for spodoptera frugiperda J. E. smith (Lepidoptera: Noctuidae)
title_full_unstemmed An optimized high-quality DNA isolation protocol for spodoptera frugiperda J. E. smith (Lepidoptera: Noctuidae)
title_short An optimized high-quality DNA isolation protocol for spodoptera frugiperda J. E. smith (Lepidoptera: Noctuidae)
title_sort optimized high quality dna isolation protocol for spodoptera frugiperda j e smith lepidoptera noctuidae
topic spodoptera frugiperda
pests of plants
dna
identification
methods
isolation techniques
plagas de plantas
identificación
url https://hdl.handle.net/10568/111747
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