Rhizosphere pH and cation‐anion balance determine the exudation of nitrification inhibitor 3‐epi‐brachialactone suggesting release via secondary transport
Biological nitrification inhibition (BNI) of Brachiaria humidicola has been attributed to nitrification-inhibiting fusicoccanes, most prominently 3-epi-brachialactone. However, its release mechanism from B. humidicola roots remains elusive. Two hydroponic experiments were performed to investigate th...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2021
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/110891 |
| _version_ | 1855513817453166592 |
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| author | Egenolf, Konrad Verma, Supriya Schöne, Jochen Klaiber, Iris Arango, Jacobo Cadisch, Georg Neumann, Günter Rasche, Frank |
| author_browse | Arango, Jacobo Cadisch, Georg Egenolf, Konrad Klaiber, Iris Neumann, Günter Rasche, Frank Schöne, Jochen Verma, Supriya |
| author_facet | Egenolf, Konrad Verma, Supriya Schöne, Jochen Klaiber, Iris Arango, Jacobo Cadisch, Georg Neumann, Günter Rasche, Frank |
| author_sort | Egenolf, Konrad |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Biological nitrification inhibition (BNI) of Brachiaria humidicola has been attributed to nitrification-inhibiting fusicoccanes, most prominently 3-epi-brachialactone. However, its release mechanism from B. humidicola roots remains elusive. Two hydroponic experiments were performed to investigate the role of rhizosphere pH and nutritional N form in regulating 3-epi-brachialactone release by B. humidicola and verify the underlying release pathway. Low rhizosphere pH and NH4 + nutrition promoted 3-epi-brachialactone exudation. However, the substitution of NH4 + by K+ revealed that the NH4 + effect was not founded in a direct physiological response to the N form but was related to the cation-anion balance during nutrient uptake. Release of 3-epi-brachialactone correlated with the transmembrane proton gradient ΔpH and NH4 + uptake (R2 = 0.92 for high ~6.8 and R2 = 0.84 for low ~4.2 trap solution pH). This corroborated the release of 3-epi-brachialactone through secondary transport, with the proton motive force (ΔP) defining transport rates across the plasma membrane. It was concluded that 3-epi-brachialactone release cannot be conceptualized as a regulated response to soil pH or NH4 + availability, but merely as the result of associated changes in ΔP. |
| format | Journal Article |
| id | CGSpace110891 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1108912025-11-11T17:39:31Z Rhizosphere pH and cation‐anion balance determine the exudation of nitrification inhibitor 3‐epi‐brachialactone suggesting release via secondary transport Egenolf, Konrad Verma, Supriya Schöne, Jochen Klaiber, Iris Arango, Jacobo Cadisch, Georg Neumann, Günter Rasche, Frank soil properties rhizosphere ph nitrification inhibitors brachiaria humidicola propiedades del suelo ph de la rizosfera inhibidores de la nitrificación cell biology genetics physiology Biological nitrification inhibition (BNI) of Brachiaria humidicola has been attributed to nitrification-inhibiting fusicoccanes, most prominently 3-epi-brachialactone. However, its release mechanism from B. humidicola roots remains elusive. Two hydroponic experiments were performed to investigate the role of rhizosphere pH and nutritional N form in regulating 3-epi-brachialactone release by B. humidicola and verify the underlying release pathway. Low rhizosphere pH and NH4 + nutrition promoted 3-epi-brachialactone exudation. However, the substitution of NH4 + by K+ revealed that the NH4 + effect was not founded in a direct physiological response to the N form but was related to the cation-anion balance during nutrient uptake. Release of 3-epi-brachialactone correlated with the transmembrane proton gradient ΔpH and NH4 + uptake (R2 = 0.92 for high ~6.8 and R2 = 0.84 for low ~4.2 trap solution pH). This corroborated the release of 3-epi-brachialactone through secondary transport, with the proton motive force (ΔP) defining transport rates across the plasma membrane. It was concluded that 3-epi-brachialactone release cannot be conceptualized as a regulated response to soil pH or NH4 + availability, but merely as the result of associated changes in ΔP. 2021-05 2021-01-18T13:10:35Z 2021-01-18T13:10:35Z Journal Article https://hdl.handle.net/10568/110891 en Open Access application/pdf Wiley Egenolf, K.; Verma, S.; Schöne, J.; Klaiber, I.; Arango, J.; Cadisch, G.; Neumann, G.; Rasche, F. (2020) Rhizosphere pH and cation‐anion balance determine the exudation of nitrification inhibitor 3‐epi‐brachialactone suggesting release via secondary transport. Physiologia Plantarum, Online first paper (06 December 2020). ISSN: 1399-3054. |
| spellingShingle | soil properties rhizosphere ph nitrification inhibitors brachiaria humidicola propiedades del suelo ph de la rizosfera inhibidores de la nitrificación cell biology genetics physiology Egenolf, Konrad Verma, Supriya Schöne, Jochen Klaiber, Iris Arango, Jacobo Cadisch, Georg Neumann, Günter Rasche, Frank Rhizosphere pH and cation‐anion balance determine the exudation of nitrification inhibitor 3‐epi‐brachialactone suggesting release via secondary transport |
| title | Rhizosphere pH and cation‐anion balance determine the exudation of nitrification inhibitor 3‐epi‐brachialactone suggesting release via secondary transport |
| title_full | Rhizosphere pH and cation‐anion balance determine the exudation of nitrification inhibitor 3‐epi‐brachialactone suggesting release via secondary transport |
| title_fullStr | Rhizosphere pH and cation‐anion balance determine the exudation of nitrification inhibitor 3‐epi‐brachialactone suggesting release via secondary transport |
| title_full_unstemmed | Rhizosphere pH and cation‐anion balance determine the exudation of nitrification inhibitor 3‐epi‐brachialactone suggesting release via secondary transport |
| title_short | Rhizosphere pH and cation‐anion balance determine the exudation of nitrification inhibitor 3‐epi‐brachialactone suggesting release via secondary transport |
| title_sort | rhizosphere ph and cation anion balance determine the exudation of nitrification inhibitor 3 epi brachialactone suggesting release via secondary transport |
| topic | soil properties rhizosphere ph nitrification inhibitors brachiaria humidicola propiedades del suelo ph de la rizosfera inhibidores de la nitrificación cell biology genetics physiology |
| url | https://hdl.handle.net/10568/110891 |
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