Metagenomic analyses and genetic diversity of Tomato leaf curl Arusha virus affecting tomato plants in Kenya
Tomato production is threatened worldwide by the occurrence of begomoviruses which are associated with tomato leaf curl diseases. There is little information on the molecular properties of tomato begomoviruses in Kenya, hence we investigated the population and genetic diversity of begomoviruses asso...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Springer
2021
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| Online Access: | https://hdl.handle.net/10568/110822 |
| _version_ | 1855518194998968320 |
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| author | Avedi, E.K. Adediji, A.O. Kilalo, D.C. Olubayo, F.M. Macharia, I. Ateka, E.M. Machuka, Eunice M. Mutuku, Josiah M. |
| author_browse | Adediji, A.O. Ateka, E.M. Avedi, E.K. Kilalo, D.C. Macharia, I. Machuka, Eunice M. Mutuku, Josiah M. Olubayo, F.M. |
| author_facet | Avedi, E.K. Adediji, A.O. Kilalo, D.C. Olubayo, F.M. Macharia, I. Ateka, E.M. Machuka, Eunice M. Mutuku, Josiah M. |
| author_sort | Avedi, E.K. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Tomato production is threatened worldwide by the occurrence of begomoviruses which are associated with tomato leaf curl diseases. There is little information on the molecular properties of tomato begomoviruses in Kenya, hence we investigated the population and genetic diversity of begomoviruses associated with tomato leaf curl in Kenya.Tomato leaf samples with virus-like symptoms were obtained from farmers’ field across the country in 2018 and Illumina sequencing undertaken to determine the genetic diversity of associated begomoviruses. Additionally, the occurrence of selection pressure and recombinant isolates within the population were also evaluated.Twelve complete begomovirus genomes were obtained from our samples with an average coverage of 99.9%. The sequences showed 95.7–99.7% identity among each other and 95.9–98.9% similarities with aTomato leaf curl virus Arusha virus(ToLCArV) isolate from Tanzania. Analysis of amino acid sequences showed the highest identities in the regions coding for the coat protein gene (98.5–100%) within the isolates, and 97.1–100% identity with the C4 gene of ToLCArV. Phylogenetic algorithms clustered all Kenyan isolates in the same clades with ToLCArV, thus confirming the isolates to be a variant of the virus. There was no evidence of recombination within our isolates. Estimation of selection pressure within the virus population revealed the occurrence of negative or purifying selection in five out of the six coding regions of the sequences.The begomovirus associated with tomato leaf curl diseases of tomato in Kenya is a variant of ToLCArV, possibly originating from Tanzania. There is low genetic diversity within the virus population and this information is useful in the development of appropriate management strategies for the disease in the country. |
| format | Journal Article |
| id | CGSpace110822 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1108222025-12-02T10:59:51Z Metagenomic analyses and genetic diversity of Tomato leaf curl Arusha virus affecting tomato plants in Kenya Avedi, E.K. Adediji, A.O. Kilalo, D.C. Olubayo, F.M. Macharia, I. Ateka, E.M. Machuka, Eunice M. Mutuku, Josiah M. tomatoes viruses genomes infectious diseases virology Tomato production is threatened worldwide by the occurrence of begomoviruses which are associated with tomato leaf curl diseases. There is little information on the molecular properties of tomato begomoviruses in Kenya, hence we investigated the population and genetic diversity of begomoviruses associated with tomato leaf curl in Kenya.Tomato leaf samples with virus-like symptoms were obtained from farmers’ field across the country in 2018 and Illumina sequencing undertaken to determine the genetic diversity of associated begomoviruses. Additionally, the occurrence of selection pressure and recombinant isolates within the population were also evaluated.Twelve complete begomovirus genomes were obtained from our samples with an average coverage of 99.9%. The sequences showed 95.7–99.7% identity among each other and 95.9–98.9% similarities with aTomato leaf curl virus Arusha virus(ToLCArV) isolate from Tanzania. Analysis of amino acid sequences showed the highest identities in the regions coding for the coat protein gene (98.5–100%) within the isolates, and 97.1–100% identity with the C4 gene of ToLCArV. Phylogenetic algorithms clustered all Kenyan isolates in the same clades with ToLCArV, thus confirming the isolates to be a variant of the virus. There was no evidence of recombination within our isolates. Estimation of selection pressure within the virus population revealed the occurrence of negative or purifying selection in five out of the six coding regions of the sequences.The begomovirus associated with tomato leaf curl diseases of tomato in Kenya is a variant of ToLCArV, possibly originating from Tanzania. There is low genetic diversity within the virus population and this information is useful in the development of appropriate management strategies for the disease in the country. 2021-12 2021-01-11T18:43:16Z 2021-01-11T18:43:16Z Journal Article https://hdl.handle.net/10568/110822 en Open Access Springer Avedi, E.K., Adediji, A.O., Kilalo, D.C., Olubayo, F.M., Macharia, I., Ateka, E.M., Machuka, E.M. and Mutuku, J.M. 2021. Metagenomic analyses and genetic diversity of Tomato leaf curl Arusha virus affecting tomato plants in Kenya. Virology Journal 18:2. |
| spellingShingle | tomatoes viruses genomes infectious diseases virology Avedi, E.K. Adediji, A.O. Kilalo, D.C. Olubayo, F.M. Macharia, I. Ateka, E.M. Machuka, Eunice M. Mutuku, Josiah M. Metagenomic analyses and genetic diversity of Tomato leaf curl Arusha virus affecting tomato plants in Kenya |
| title | Metagenomic analyses and genetic diversity of Tomato leaf curl Arusha virus affecting tomato plants in Kenya |
| title_full | Metagenomic analyses and genetic diversity of Tomato leaf curl Arusha virus affecting tomato plants in Kenya |
| title_fullStr | Metagenomic analyses and genetic diversity of Tomato leaf curl Arusha virus affecting tomato plants in Kenya |
| title_full_unstemmed | Metagenomic analyses and genetic diversity of Tomato leaf curl Arusha virus affecting tomato plants in Kenya |
| title_short | Metagenomic analyses and genetic diversity of Tomato leaf curl Arusha virus affecting tomato plants in Kenya |
| title_sort | metagenomic analyses and genetic diversity of tomato leaf curl arusha virus affecting tomato plants in kenya |
| topic | tomatoes viruses genomes infectious diseases virology |
| url | https://hdl.handle.net/10568/110822 |
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