Synergistic effect of two nanotechnologies enhances the protective capacity of the Theileria parva sporozoite p67C antigen in cattle
East Coast fever (ECF), caused by Theileria parva, is the most important tick-borne disease of cattle in sub-Saharan Africa. Practical disadvantages associated with the currently used live-parasite vaccine could be overcome by subunit vaccines. An 80-aa polypeptide derived from the C-terminal portio...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
American Association of Immunologists
2021
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/110813 |
| _version_ | 1855525199520202752 |
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| author | Lacasta, Anna Mody, K.T. Goeyse, I. de Yu, C. Zhang, J. Nyagwange, James Mwalimu, Stephen Awino, Elias Saya, Rosemary M. Njoroge, Thomas Muriuki, Robert Ndiwa, Nicholas N. Poole, Elizabeth J. Zhang, B. Cavallaro, A. Mahony, T.J. Steinaa, Lucilla Mitter, N. Nene, Vishvanath M. |
| author_browse | Awino, Elias Cavallaro, A. Goeyse, I. de Lacasta, Anna Mahony, T.J. Mitter, N. Mody, K.T. Muriuki, Robert Mwalimu, Stephen Ndiwa, Nicholas N. Nene, Vishvanath M. Njoroge, Thomas Nyagwange, James Poole, Elizabeth J. Saya, Rosemary M. Steinaa, Lucilla Yu, C. Zhang, B. Zhang, J. |
| author_facet | Lacasta, Anna Mody, K.T. Goeyse, I. de Yu, C. Zhang, J. Nyagwange, James Mwalimu, Stephen Awino, Elias Saya, Rosemary M. Njoroge, Thomas Muriuki, Robert Ndiwa, Nicholas N. Poole, Elizabeth J. Zhang, B. Cavallaro, A. Mahony, T.J. Steinaa, Lucilla Mitter, N. Nene, Vishvanath M. |
| author_sort | Lacasta, Anna |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | East Coast fever (ECF), caused by Theileria parva, is the most important tick-borne disease of cattle in sub-Saharan Africa. Practical disadvantages associated with the currently used live-parasite vaccine could be overcome by subunit vaccines. An 80-aa polypeptide derived from the C-terminal portion of p67, a sporozoite surface Ag and target of neutralizing Abs, was the focus of the efforts on subunit vaccines against ECF and subjected to several vaccine trials with very promising results. However, the vaccination regimen was far from optimized, involving three inoculations of 450 μg of soluble p67C (s-p67C) Ag formulated in the Seppic adjuvant Montanide ISA 206 VG. Hence, an improved formulation of this polypeptide Ag is needed. In this study, we report on two nanotechnologies that enhance the bovine immune responses to p67C. Individually, HBcAg-p67C (chimeric hepatitis B core Ag virus-like particles displaying p67C) and silica vesicle (SV)-p67C (s-p67C adsorbed to SV-140-C18, octadecyl-modified SVs) adjuvanted with ISA 206 VG primed strong Ab and T cell responses to p67C in cattle, respectively. Coimmunization of cattle (Bos taurus) with HBcAg-p67C and SV-p67C resulted in stimulation of both high Ab titers and CD4 T cell response to p67C, leading to the highest subunit vaccine efficacy we have achieved to date with the p67C immunogen. These results offer the much-needed research depth on the innovative platforms for developing effective novel protein-based bovine vaccines to further the advancement. |
| format | Journal Article |
| id | CGSpace110813 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | American Association of Immunologists |
| publisherStr | American Association of Immunologists |
| record_format | dspace |
| spelling | CGSpace1108132025-12-08T09:54:28Z Synergistic effect of two nanotechnologies enhances the protective capacity of the Theileria parva sporozoite p67C antigen in cattle Lacasta, Anna Mody, K.T. Goeyse, I. de Yu, C. Zhang, J. Nyagwange, James Mwalimu, Stephen Awino, Elias Saya, Rosemary M. Njoroge, Thomas Muriuki, Robert Ndiwa, Nicholas N. Poole, Elizabeth J. Zhang, B. Cavallaro, A. Mahony, T.J. Steinaa, Lucilla Mitter, N. Nene, Vishvanath M. east coast fever animal diseases nanotechnology immunology East Coast fever (ECF), caused by Theileria parva, is the most important tick-borne disease of cattle in sub-Saharan Africa. Practical disadvantages associated with the currently used live-parasite vaccine could be overcome by subunit vaccines. An 80-aa polypeptide derived from the C-terminal portion of p67, a sporozoite surface Ag and target of neutralizing Abs, was the focus of the efforts on subunit vaccines against ECF and subjected to several vaccine trials with very promising results. However, the vaccination regimen was far from optimized, involving three inoculations of 450 μg of soluble p67C (s-p67C) Ag formulated in the Seppic adjuvant Montanide ISA 206 VG. Hence, an improved formulation of this polypeptide Ag is needed. In this study, we report on two nanotechnologies that enhance the bovine immune responses to p67C. Individually, HBcAg-p67C (chimeric hepatitis B core Ag virus-like particles displaying p67C) and silica vesicle (SV)-p67C (s-p67C adsorbed to SV-140-C18, octadecyl-modified SVs) adjuvanted with ISA 206 VG primed strong Ab and T cell responses to p67C in cattle, respectively. Coimmunization of cattle (Bos taurus) with HBcAg-p67C and SV-p67C resulted in stimulation of both high Ab titers and CD4 T cell response to p67C, leading to the highest subunit vaccine efficacy we have achieved to date with the p67C immunogen. These results offer the much-needed research depth on the innovative platforms for developing effective novel protein-based bovine vaccines to further the advancement. 2021-02-15 2021-01-11T10:11:55Z 2021-01-11T10:11:55Z Journal Article https://hdl.handle.net/10568/110813 en Open Access American Association of Immunologists Lacasta, A., Mody, K.T., Goeyse, I. De, Yu, C., Zhang, J., Nyagwange, J., Mwalimu, S., Awino, E., Saya, R., Njoroge, T., Muriuki, R., Ndiwa, N., Poole, E.J., Zhang, B., Cavallaro, A., Mahony, T.J., Steinaa, L., Mitter, N. and Nene, V. 2021. Synergistic effect of two nanotechnologies enhances the protective capacity of the Theileria parva sporozoite p67C antigen in cattle. Journal of Immunology 206(4): 686–699. |
| spellingShingle | east coast fever animal diseases nanotechnology immunology Lacasta, Anna Mody, K.T. Goeyse, I. de Yu, C. Zhang, J. Nyagwange, James Mwalimu, Stephen Awino, Elias Saya, Rosemary M. Njoroge, Thomas Muriuki, Robert Ndiwa, Nicholas N. Poole, Elizabeth J. Zhang, B. Cavallaro, A. Mahony, T.J. Steinaa, Lucilla Mitter, N. Nene, Vishvanath M. Synergistic effect of two nanotechnologies enhances the protective capacity of the Theileria parva sporozoite p67C antigen in cattle |
| title | Synergistic effect of two nanotechnologies enhances the protective capacity of the Theileria parva sporozoite p67C antigen in cattle |
| title_full | Synergistic effect of two nanotechnologies enhances the protective capacity of the Theileria parva sporozoite p67C antigen in cattle |
| title_fullStr | Synergistic effect of two nanotechnologies enhances the protective capacity of the Theileria parva sporozoite p67C antigen in cattle |
| title_full_unstemmed | Synergistic effect of two nanotechnologies enhances the protective capacity of the Theileria parva sporozoite p67C antigen in cattle |
| title_short | Synergistic effect of two nanotechnologies enhances the protective capacity of the Theileria parva sporozoite p67C antigen in cattle |
| title_sort | synergistic effect of two nanotechnologies enhances the protective capacity of the theileria parva sporozoite p67c antigen in cattle |
| topic | east coast fever animal diseases nanotechnology immunology |
| url | https://hdl.handle.net/10568/110813 |
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