Genetic basis of maize resistance to multiple insect pests: integrated genome-wide comparative mapping and candidate gene prioritization
Several species of herbivores feed on maize in field and storage setups, making the development of multiple insect resistance a critical breeding target. In this study, an association mapping panel of 341 tropical maize lines was evaluated in three field environments for resistance to fall armyworm...
| Autores principales: | , , , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2020
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/110812 |
| _version_ | 1855514157236879360 |
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| author | Badji, Arfang Kwemoi, Daniel Bomet Machida, Lewis Okii, Dennis Mwila, Natasha Agbahoungba, Symphorien Kumi, Frank Ibanda, Angele Bararyenya, Astere Solemanegy, Marta Odong, Thomas L. Wasswa, Peter Otim, Michael Asea, Godfrey Ochwo-Ssemakula, Mildred Talwana, Herbert A.L. Kyamanywa, Samuel Rubaihayo, Patrick |
| author_browse | Agbahoungba, Symphorien Asea, Godfrey Badji, Arfang Bararyenya, Astere Ibanda, Angele Kumi, Frank Kwemoi, Daniel Bomet Kyamanywa, Samuel Machida, Lewis Mwila, Natasha Ochwo-Ssemakula, Mildred Odong, Thomas L. Okii, Dennis Otim, Michael Rubaihayo, Patrick Solemanegy, Marta Talwana, Herbert A.L. Wasswa, Peter |
| author_facet | Badji, Arfang Kwemoi, Daniel Bomet Machida, Lewis Okii, Dennis Mwila, Natasha Agbahoungba, Symphorien Kumi, Frank Ibanda, Angele Bararyenya, Astere Solemanegy, Marta Odong, Thomas L. Wasswa, Peter Otim, Michael Asea, Godfrey Ochwo-Ssemakula, Mildred Talwana, Herbert A.L. Kyamanywa, Samuel Rubaihayo, Patrick |
| author_sort | Badji, Arfang |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Several species of herbivores feed on maize in field and storage setups, making the development of multiple insect resistance a critical breeding target. In this study, an association mapping panel of 341 tropical maize lines was evaluated in three field environments for resistance to fall armyworm (FAW), whilst bulked grains were subjected to a maize weevil (MW) bioassay and genotyped with Diversity Array Technology’s single nucleotide polymorphisms (SNPs) markers.
A multi-locus genome-wide association study (GWAS) revealed 62 quantitative trait nucleotides (QTNs) associated with FAW and MW resistance traits on all 10 maize chromosomes, of which, 47 and 31 were discovered at stringent Bonferroni genome-wide significance levels of 0.05 and 0.01, respectively, and located within or close to multiple insect resistance genomic regions (MIRGRs) concerning FAW, SB, and MW. Sixteen QTNs influenced multiple traits, of which, six were associated with resistance to both FAWandMW, suggesting a pleiotropic genetic control. Functional prioritization of candidate genes (CGs) located within 10–30 kb of the QTNs revealed 64 putative GWAS-based CGs (GbCGs) showing evidence of involvement in plant defense mechanisms. Only one GbCG was associated with each of the five of the six combined resistance QTNs, thus reinforcing the pleiotropy hypothesis. In addition, through in silico co-functional network inferences, an additional 107 network-based CGs (NbCGs), biologically connected to the 64 GbCGs, and di erentially expressed under biotic or abiotic stress, were revealed within MIRGRs. The provided multiple insect resistance physical map should contribute to the development of combined insect resistance in maize. |
| format | Journal Article |
| id | CGSpace110812 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1108122025-12-08T09:54:28Z Genetic basis of maize resistance to multiple insect pests: integrated genome-wide comparative mapping and candidate gene prioritization Badji, Arfang Kwemoi, Daniel Bomet Machida, Lewis Okii, Dennis Mwila, Natasha Agbahoungba, Symphorien Kumi, Frank Ibanda, Angele Bararyenya, Astere Solemanegy, Marta Odong, Thomas L. Wasswa, Peter Otim, Michael Asea, Godfrey Ochwo-Ssemakula, Mildred Talwana, Herbert A.L. Kyamanywa, Samuel Rubaihayo, Patrick pest insects genomes pest resistance control methods iinsectos dañinos genomas resistencia a las plagas genetics Several species of herbivores feed on maize in field and storage setups, making the development of multiple insect resistance a critical breeding target. In this study, an association mapping panel of 341 tropical maize lines was evaluated in three field environments for resistance to fall armyworm (FAW), whilst bulked grains were subjected to a maize weevil (MW) bioassay and genotyped with Diversity Array Technology’s single nucleotide polymorphisms (SNPs) markers. A multi-locus genome-wide association study (GWAS) revealed 62 quantitative trait nucleotides (QTNs) associated with FAW and MW resistance traits on all 10 maize chromosomes, of which, 47 and 31 were discovered at stringent Bonferroni genome-wide significance levels of 0.05 and 0.01, respectively, and located within or close to multiple insect resistance genomic regions (MIRGRs) concerning FAW, SB, and MW. Sixteen QTNs influenced multiple traits, of which, six were associated with resistance to both FAWandMW, suggesting a pleiotropic genetic control. Functional prioritization of candidate genes (CGs) located within 10–30 kb of the QTNs revealed 64 putative GWAS-based CGs (GbCGs) showing evidence of involvement in plant defense mechanisms. Only one GbCG was associated with each of the five of the six combined resistance QTNs, thus reinforcing the pleiotropy hypothesis. In addition, through in silico co-functional network inferences, an additional 107 network-based CGs (NbCGs), biologically connected to the 64 GbCGs, and di erentially expressed under biotic or abiotic stress, were revealed within MIRGRs. The provided multiple insect resistance physical map should contribute to the development of combined insect resistance in maize. 2020-12 2021-01-11T08:49:27Z 2021-01-11T08:49:27Z Journal Article https://hdl.handle.net/10568/110812 en Open Access application/pdf MDPI Badji, A.; Kwemoi, D.; Bomet; Machida, L.; Okii, D.; Mwila, N.; Agbahoungba, S.; Kumi, F.; Ibanda, A.; Bararyenya, A.; Solemanegy, M.; Odong , T.; Wasswa, P.; Otim, M.; Asea, G.; Ochwo-Ssemakula, M.; Talwana, H.; Kyamanywa, S.; Rubaihayo, P. (2020) Genetic basis of maize resistance to multiple insect pests: integrated genome-wide comparative mapping and candidate gene prioritization. Genes 11(6) p. 689 ISSN: 2073-4425 |
| spellingShingle | pest insects genomes pest resistance control methods iinsectos dañinos genomas resistencia a las plagas genetics Badji, Arfang Kwemoi, Daniel Bomet Machida, Lewis Okii, Dennis Mwila, Natasha Agbahoungba, Symphorien Kumi, Frank Ibanda, Angele Bararyenya, Astere Solemanegy, Marta Odong, Thomas L. Wasswa, Peter Otim, Michael Asea, Godfrey Ochwo-Ssemakula, Mildred Talwana, Herbert A.L. Kyamanywa, Samuel Rubaihayo, Patrick Genetic basis of maize resistance to multiple insect pests: integrated genome-wide comparative mapping and candidate gene prioritization |
| title | Genetic basis of maize resistance to multiple insect pests: integrated genome-wide comparative mapping and candidate gene prioritization |
| title_full | Genetic basis of maize resistance to multiple insect pests: integrated genome-wide comparative mapping and candidate gene prioritization |
| title_fullStr | Genetic basis of maize resistance to multiple insect pests: integrated genome-wide comparative mapping and candidate gene prioritization |
| title_full_unstemmed | Genetic basis of maize resistance to multiple insect pests: integrated genome-wide comparative mapping and candidate gene prioritization |
| title_short | Genetic basis of maize resistance to multiple insect pests: integrated genome-wide comparative mapping and candidate gene prioritization |
| title_sort | genetic basis of maize resistance to multiple insect pests integrated genome wide comparative mapping and candidate gene prioritization |
| topic | pest insects genomes pest resistance control methods iinsectos dañinos genomas resistencia a las plagas genetics |
| url | https://hdl.handle.net/10568/110812 |
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