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Identification of novel lncrnas differentially expressed in placentas of Chinese Ningqiang pony and Yili horse breeds

As a nutrient sensor, the placenta plays a key role in regulating fetus growth and development. Long non-coding RNAs (lncRNAs) have been shown to regulate growth-related traits. However, the biological function of lncRNAs in horse placentas remains unclear. To compare the expression patterns of lncR...

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Main Authors: Pu, Y., Zhang, Y., Zhang, T., Han Jianlin, Ma, Y., Liu, X.
Format: Journal Article
Language:Inglés
Published: MDPI 2020
Subjects:
animal breeding
genetics
ponies
horses
Online Access:https://hdl.handle.net/10568/110664
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author Pu, Y.
Zhang, Y.
Zhang, T.
Han Jianlin
Ma, Y.
Liu, X.
author_browse Han Jianlin
Liu, X.
Ma, Y.
Pu, Y.
Zhang, T.
Zhang, Y.
author_facet Pu, Y.
Zhang, Y.
Zhang, T.
Han Jianlin
Ma, Y.
Liu, X.
author_sort Pu, Y.
collection Repository of Agricultural Research Outputs (CGSpace)
description As a nutrient sensor, the placenta plays a key role in regulating fetus growth and development. Long non-coding RNAs (lncRNAs) have been shown to regulate growth-related traits. However, the biological function of lncRNAs in horse placentas remains unclear. To compare the expression patterns of lncRNAs in the placentas of the Chinese Ningqiang (NQ) and Yili (YL) breeds, we performed a transcriptome analysis using RNA sequencing (RNA-seq) technology. NQ is a pony breed with an average adult height at the withers of less than 106 cm, whereas that of YL is around 148 cm. Based on 813 million high-quality reads and stringent quality control procedures, 3011 transcripts coding for 1464 placental lncRNAs were identified and mapped to the horse reference genome. We found 107 differentially expressed lncRNAs (DELs) between NQ and YL, including 68 up-regulated and 39 down-regulated DELs in YL. Six (TBX3, CACNA1F, EDN3, KAT5, ZNF281, TMED2, and TGFB1) out of the 233 genes targeted by DELs were identified as being involved in limb development, skeletal myoblast differentiation, and embryo development. Two DELs were predicted to target the TBX3 gene, which was found to be under strong selection and associated with small body size in the Chinese Debao pony breed. This finding suggests the potential functional significance of placental lncRNAs in regulating horse body size.
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language Inglés
publishDate 2020
publishDateRange 2020
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publisher MDPI
publisherStr MDPI
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spelling CGSpace1106642024-05-01T08:18:42Z Identification of novel lncrnas differentially expressed in placentas of Chinese Ningqiang pony and Yili horse breeds Pu, Y. Zhang, Y. Zhang, T. Han Jianlin Ma, Y. Liu, X. animal breeding genetics ponies horses As a nutrient sensor, the placenta plays a key role in regulating fetus growth and development. Long non-coding RNAs (lncRNAs) have been shown to regulate growth-related traits. However, the biological function of lncRNAs in horse placentas remains unclear. To compare the expression patterns of lncRNAs in the placentas of the Chinese Ningqiang (NQ) and Yili (YL) breeds, we performed a transcriptome analysis using RNA sequencing (RNA-seq) technology. NQ is a pony breed with an average adult height at the withers of less than 106 cm, whereas that of YL is around 148 cm. Based on 813 million high-quality reads and stringent quality control procedures, 3011 transcripts coding for 1464 placental lncRNAs were identified and mapped to the horse reference genome. We found 107 differentially expressed lncRNAs (DELs) between NQ and YL, including 68 up-regulated and 39 down-regulated DELs in YL. Six (TBX3, CACNA1F, EDN3, KAT5, ZNF281, TMED2, and TGFB1) out of the 233 genes targeted by DELs were identified as being involved in limb development, skeletal myoblast differentiation, and embryo development. Two DELs were predicted to target the TBX3 gene, which was found to be under strong selection and associated with small body size in the Chinese Debao pony breed. This finding suggests the potential functional significance of placental lncRNAs in regulating horse body size. 2020-01-11 2020-12-29T09:24:00Z 2020-12-29T09:24:00Z Journal Article https://hdl.handle.net/10568/110664 en Open Access MDPI Pu, Y., Zhang, Y., Zhang, T., Han, J., Ma, Y. and Liu, X. 2020. Identification of novel lncrnas differentially expressed in placentas of Chinese Ningqiang pony and Yili horse breeds. Animals 10:119.
spellingShingle animal breeding
genetics
ponies
horses
Pu, Y.
Zhang, Y.
Zhang, T.
Han Jianlin
Ma, Y.
Liu, X.
Identification of novel lncrnas differentially expressed in placentas of Chinese Ningqiang pony and Yili horse breeds
title Identification of novel lncrnas differentially expressed in placentas of Chinese Ningqiang pony and Yili horse breeds
title_full Identification of novel lncrnas differentially expressed in placentas of Chinese Ningqiang pony and Yili horse breeds
title_fullStr Identification of novel lncrnas differentially expressed in placentas of Chinese Ningqiang pony and Yili horse breeds
title_full_unstemmed Identification of novel lncrnas differentially expressed in placentas of Chinese Ningqiang pony and Yili horse breeds
title_short Identification of novel lncrnas differentially expressed in placentas of Chinese Ningqiang pony and Yili horse breeds
title_sort identification of novel lncrnas differentially expressed in placentas of chinese ningqiang pony and yili horse breeds
topic animal breeding
genetics
ponies
horses
url https://hdl.handle.net/10568/110664
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