Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia
Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia in cattle. A prototype subunit vaccine is being developed, however, there is currently no diagnostic test that can differentiate between infected cattle and those vaccinated with the prototype subun...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/110650 |
| _version_ | 1855521288475377664 |
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| author | Lutta, H.O. Odongo, D. Mather, A. Pérez Casal, J. Potter, A. Gerdts, V. Berberov, E.M. Kyallo, Martina M. Kipronoh, A. Olum, M. Pelle, Roger Naessens, Jan |
| author_browse | Berberov, E.M. Gerdts, V. Kipronoh, A. Kyallo, Martina M. Lutta, H.O. Mather, A. Naessens, Jan Odongo, D. Olum, M. Pelle, Roger Potter, A. Pérez Casal, J. |
| author_facet | Lutta, H.O. Odongo, D. Mather, A. Pérez Casal, J. Potter, A. Gerdts, V. Berberov, E.M. Kyallo, Martina M. Kipronoh, A. Olum, M. Pelle, Roger Naessens, Jan |
| author_sort | Lutta, H.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia in cattle. A prototype subunit vaccine is being developed, however, there is currently no diagnostic test that can differentiate between infected cattle and those vaccinated with the prototype subunit vaccine. This study characterized Mmm proteins to identify potential antigens for use in differentiating infected from vaccinated animals.Ten Mmm antigens expressed as recombinant proteins were tested in an indirect ELISA using experimental sera from control groups, infected, and vaccinated animals. Data were imported into R software for analysis and drawing of the box and scatter plots while Cohen’s Kappa assessed the level of agreement between the Mmm antigens. Two vaccine antigens (MSC_0499 and MSC_0776) were superior in detecting antibodies in sera of animals vaccinated with the subunit vaccines while two non-vaccine antigens (MSC_0636 and LppB) detected antibodies in sera of infected animals showing all clinical stages of the disease. Sensitivity and specificity of above 87.5% were achieved when the MSC_0499 and MSC_0636 antigens were tested on sera from vaccinated and infected animals.The MSC_0499 and MSC_0776 antigens were the most promising for detecting vaccinated animals, while MSC_0636 and LppB were the best targets to identify infected animals. Further testing of sera from vaccinated and infected animals collected at different time intervals in the field should help establish how useful a diagnostic test based on a cocktail of these proteins would be. |
| format | Journal Article |
| id | CGSpace110650 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1106502024-05-01T08:16:28Z Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia Lutta, H.O. Odongo, D. Mather, A. Pérez Casal, J. Potter, A. Gerdts, V. Berberov, E.M. Kyallo, Martina M. Kipronoh, A. Olum, M. Pelle, Roger Naessens, Jan cattle antigens livestock animal diseases Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia in cattle. A prototype subunit vaccine is being developed, however, there is currently no diagnostic test that can differentiate between infected cattle and those vaccinated with the prototype subunit vaccine. This study characterized Mmm proteins to identify potential antigens for use in differentiating infected from vaccinated animals.Ten Mmm antigens expressed as recombinant proteins were tested in an indirect ELISA using experimental sera from control groups, infected, and vaccinated animals. Data were imported into R software for analysis and drawing of the box and scatter plots while Cohen’s Kappa assessed the level of agreement between the Mmm antigens. Two vaccine antigens (MSC_0499 and MSC_0776) were superior in detecting antibodies in sera of animals vaccinated with the subunit vaccines while two non-vaccine antigens (MSC_0636 and LppB) detected antibodies in sera of infected animals showing all clinical stages of the disease. Sensitivity and specificity of above 87.5% were achieved when the MSC_0499 and MSC_0636 antigens were tested on sera from vaccinated and infected animals.The MSC_0499 and MSC_0776 antigens were the most promising for detecting vaccinated animals, while MSC_0636 and LppB were the best targets to identify infected animals. Further testing of sera from vaccinated and infected animals collected at different time intervals in the field should help establish how useful a diagnostic test based on a cocktail of these proteins would be. 2020-12 2020-12-27T14:26:36Z 2020-12-27T14:26:36Z Journal Article https://hdl.handle.net/10568/110650 en Open Access Springer Lutta, H.O., Odongo, D., Mather, A., Perez-Casal, J., Perez-Casal, J., Potter, A., Gerdts, V., Berberov, E.M., Kyallo, M., Kipronoh, A., Olum, M., Pelle, R., Naessens, J. 2020. Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia. BMC Veterinary Research 16:236. |
| spellingShingle | cattle antigens livestock animal diseases Lutta, H.O. Odongo, D. Mather, A. Pérez Casal, J. Potter, A. Gerdts, V. Berberov, E.M. Kyallo, Martina M. Kipronoh, A. Olum, M. Pelle, Roger Naessens, Jan Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia |
| title | Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia |
| title_full | Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia |
| title_fullStr | Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia |
| title_full_unstemmed | Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia |
| title_short | Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia |
| title_sort | baseline analysis of mycoplasma mycoides subsp mycoides antigens as targets for a diva assay for use with a subunit vaccine for contagious bovine pleuropneumonia |
| topic | cattle antigens livestock animal diseases |
| url | https://hdl.handle.net/10568/110650 |
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