Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits

Understanding the genetic changes underlying phenotypic variation in sheep (Ovis aries) may facilitate our efforts towards further improvement. Here, we report the deep resequencing of 248 sheep including the wild ancestor (O. orientalis), landraces, and improved breeds. We explored the sheep variom...

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Main Authors: Li, X., Yang, J., Shen, M., Xie, X-L., Liu, G-J., Xu, Y-X., Lv, F-H., Yang, H., Yang, Y-L., Liu, C-B., Zhou, P., Wan, P-C., Zhang, Y-S., Gao, L., Yang, J-Q., Pi, W-H., Ren, Y-L., Shen, Z-Q., Wang, F., Deng, J., Xu, S.S., Salehian-Dehkordi, H., Hehua, E., Esmailizadeh, A., Dehghani-Qanatqestani, M., Štěpánek, O., Weimann, C., Erhardt, G., Amane, A., Mwacharo, Joram M., Han Jianlin, Hanotte, Olivier H., Lenstra, J.A., Kantanen, J., Coltman, D.W., Kijas, J.W., Bruford, M.W., Periasamy, K., Wang, X-H., Li, M-H.
Format: Journal Article
Language:Inglés
Published: Springer 2020
Subjects:
Online Access:https://hdl.handle.net/10568/110647
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author Li, X.
Yang, J.
Shen, M.
Xie, X-L.
Liu, G-J.
Xu, Y-X.
Lv, F-H.
Yang, H.
Yang, Y-L.
Liu, C-B.
Zhou, P.
Wan, P-C.
Zhang, Y-S.
Gao, L.
Yang, J-Q.
Pi, W-H.
Ren, Y-L.
Shen, Z-Q.
Wang, F.
Deng, J.
Xu, S.S.
Salehian-Dehkordi, H.
Hehua, E.
Esmailizadeh, A.
Dehghani-Qanatqestani, M.
Štěpánek, O.
Weimann, C.
Erhardt, G.
Amane, A.
Mwacharo, Joram M.
Han Jianlin
Hanotte, Olivier H.
Lenstra, J.A.
Kantanen, J.
Coltman, D.W.
Kijas, J.W.
Bruford, M.W.
Periasamy, K.
Wang, X-H.
Li, M-H.
author_browse Amane, A.
Bruford, M.W.
Coltman, D.W.
Dehghani-Qanatqestani, M.
Deng, J.
Erhardt, G.
Esmailizadeh, A.
Gao, L.
Han Jianlin
Hanotte, Olivier H.
Hehua, E.
Kantanen, J.
Kijas, J.W.
Lenstra, J.A.
Li, M-H.
Li, X.
Liu, C-B.
Liu, G-J.
Lv, F-H.
Mwacharo, Joram M.
Periasamy, K.
Pi, W-H.
Ren, Y-L.
Salehian-Dehkordi, H.
Shen, M.
Shen, Z-Q.
Wan, P-C.
Wang, F.
Wang, X-H.
Weimann, C.
Xie, X-L.
Xu, S.S.
Xu, Y-X.
Yang, H.
Yang, J-Q.
Yang, J.
Yang, Y-L.
Zhang, Y-S.
Zhou, P.
Štěpánek, O.
author_facet Li, X.
Yang, J.
Shen, M.
Xie, X-L.
Liu, G-J.
Xu, Y-X.
Lv, F-H.
Yang, H.
Yang, Y-L.
Liu, C-B.
Zhou, P.
Wan, P-C.
Zhang, Y-S.
Gao, L.
Yang, J-Q.
Pi, W-H.
Ren, Y-L.
Shen, Z-Q.
Wang, F.
Deng, J.
Xu, S.S.
Salehian-Dehkordi, H.
Hehua, E.
Esmailizadeh, A.
Dehghani-Qanatqestani, M.
Štěpánek, O.
Weimann, C.
Erhardt, G.
Amane, A.
Mwacharo, Joram M.
Han Jianlin
Hanotte, Olivier H.
Lenstra, J.A.
Kantanen, J.
Coltman, D.W.
Kijas, J.W.
Bruford, M.W.
Periasamy, K.
Wang, X-H.
Li, M-H.
author_sort Li, X.
collection Repository of Agricultural Research Outputs (CGSpace)
description Understanding the genetic changes underlying phenotypic variation in sheep (Ovis aries) may facilitate our efforts towards further improvement. Here, we report the deep resequencing of 248 sheep including the wild ancestor (O. orientalis), landraces, and improved breeds. We explored the sheep variome and selection signatures. We detected genomic regions harboring genes associated with distinct morphological and agronomic traits, which may be past and potential future targets of domestication, breeding, and selection. Furthermore, we found non-synonymous mutations in a set of plausible candidate genes and significant differences in their allele frequency distributions across breeds. We identified PDGFD as a likely causal gene for fat deposition in the tails of sheep through transcriptome, RT-PCR, qPCR, and Western blot analyses. Our results provide insights into the demographic history of sheep and a valuable genomic resource for future genetic studies and improved genome-assisted breeding of sheep and other domestic animals.
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spelling CGSpace1106472025-12-08T09:54:28Z Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits Li, X. Yang, J. Shen, M. Xie, X-L. Liu, G-J. Xu, Y-X. Lv, F-H. Yang, H. Yang, Y-L. Liu, C-B. Zhou, P. Wan, P-C. Zhang, Y-S. Gao, L. Yang, J-Q. Pi, W-H. Ren, Y-L. Shen, Z-Q. Wang, F. Deng, J. Xu, S.S. Salehian-Dehkordi, H. Hehua, E. Esmailizadeh, A. Dehghani-Qanatqestani, M. Štěpánek, O. Weimann, C. Erhardt, G. Amane, A. Mwacharo, Joram M. Han Jianlin Hanotte, Olivier H. Lenstra, J.A. Kantanen, J. Coltman, D.W. Kijas, J.W. Bruford, M.W. Periasamy, K. Wang, X-H. Li, M-H. genomics genetics sheep agronomic characters animal health small ruminants Understanding the genetic changes underlying phenotypic variation in sheep (Ovis aries) may facilitate our efforts towards further improvement. Here, we report the deep resequencing of 248 sheep including the wild ancestor (O. orientalis), landraces, and improved breeds. We explored the sheep variome and selection signatures. We detected genomic regions harboring genes associated with distinct morphological and agronomic traits, which may be past and potential future targets of domestication, breeding, and selection. Furthermore, we found non-synonymous mutations in a set of plausible candidate genes and significant differences in their allele frequency distributions across breeds. We identified PDGFD as a likely causal gene for fat deposition in the tails of sheep through transcriptome, RT-PCR, qPCR, and Western blot analyses. Our results provide insights into the demographic history of sheep and a valuable genomic resource for future genetic studies and improved genome-assisted breeding of sheep and other domestic animals. 2020-06-04 2020-12-27T14:25:15Z 2020-12-27T14:25:15Z Journal Article https://hdl.handle.net/10568/110647 en Open Access Springer Li, X., Yang, J., Shen, M., Xie, X-L., Liu, G-J., Xu, Y-X., Lv, F-H., Yang, H., Yang, Y-L., Liu, C-B., Zhou, P., Wan, P-C., Zhang, Y-S., Gao, L., Yang, J-Q., Pi, W-H., Ren, Y-L., Shen, Z-Q., Wang, F., Deng, J., Xu, S.S., Salehian-Dehkordi, H., Hehua, E., Esmailizadeh, A., Dehghani-Qanatqestani, M., Štěpánek, O., Weimann, C., Erhardt, G., Amane, A., Mwacharo, Joram M., Jianlin, Han, Hanotte, O.H., Lenstra, J.A., Kantanen, J., Coltman, D.W., Kijas, J.W., Bruford, M.W., Periasamy, K., Wang, X-H. and Li, M-H. 2020. Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits. Nature Communications 11:2815.
spellingShingle genomics
genetics
sheep
agronomic characters
animal health
small ruminants
Li, X.
Yang, J.
Shen, M.
Xie, X-L.
Liu, G-J.
Xu, Y-X.
Lv, F-H.
Yang, H.
Yang, Y-L.
Liu, C-B.
Zhou, P.
Wan, P-C.
Zhang, Y-S.
Gao, L.
Yang, J-Q.
Pi, W-H.
Ren, Y-L.
Shen, Z-Q.
Wang, F.
Deng, J.
Xu, S.S.
Salehian-Dehkordi, H.
Hehua, E.
Esmailizadeh, A.
Dehghani-Qanatqestani, M.
Štěpánek, O.
Weimann, C.
Erhardt, G.
Amane, A.
Mwacharo, Joram M.
Han Jianlin
Hanotte, Olivier H.
Lenstra, J.A.
Kantanen, J.
Coltman, D.W.
Kijas, J.W.
Bruford, M.W.
Periasamy, K.
Wang, X-H.
Li, M-H.
Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits
title Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits
title_full Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits
title_fullStr Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits
title_full_unstemmed Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits
title_short Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits
title_sort whole genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits
topic genomics
genetics
sheep
agronomic characters
animal health
small ruminants
url https://hdl.handle.net/10568/110647
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