Assessment of fungal contamination in fish feed from the Lake Victoria Basin, Uganda
The emergence of commercial fish farming has stimulated the establishment of fish feed factories in Uganda. However, no information is available on the safety of the feed, mainly due to lack of mycotoxin testing facilities and weak regulatory systems. A study was carried out to examine fungal coloni...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2020
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/110624 |
| _version_ | 1855534079376621568 |
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| author | Namulawa, V.T. Mutiga, Samuel K. Musimbi, F. Akello, S. Ng'ang'a, Fredrick M. Kago, Leah Kyallo, Martina M. Harvey, Jagger J.W. Ghimire, Sita R. |
| author_browse | Akello, S. Ghimire, Sita R. Harvey, Jagger J.W. Kago, Leah Kyallo, Martina M. Musimbi, F. Mutiga, Samuel K. Namulawa, V.T. Ng'ang'a, Fredrick M. |
| author_facet | Namulawa, V.T. Mutiga, Samuel K. Musimbi, F. Akello, S. Ng'ang'a, Fredrick M. Kago, Leah Kyallo, Martina M. Harvey, Jagger J.W. Ghimire, Sita R. |
| author_sort | Namulawa, V.T. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The emergence of commercial fish farming has stimulated the establishment of fish feed factories in Uganda. However, no information is available on the safety of the feed, mainly due to lack of mycotoxin testing facilities and weak regulatory systems. A study was carried out to examine fungal colonization and mycotoxin contamination in fish feed samples (n = 147) of different types collected from nine fish farms (n = 81) and seven fish feed factories (n = 66) in the Lake Victoria Basin (LVB). Fungi were isolated in potato dextrose agar, grouped into morphotypes and representative isolates from each morphotype were identified based on the internal transcribed spacer (ITS) region of ribosomal DNA sequences. Aflatoxin B1 (AFB1) and total fumonisin (combinations of B1, B2 and B3; hereinafter named fumonisin) levels in feed samples were determined by enzyme-linked immunosorbent assay (ELISA). A wide range of fungi, including toxigenic Aspergillus flavus and Fusarium verticillioides, were isolated from the fish feed samples. AFB1 was detected in 48% of the factory samples and in 63% of the farm samples, with toxin levels <40 and >400 µg/kg, respectively. Similarly, 31% of the factory samples and 29% of the farm samples had fumonisin contamination ranging between 0.1 and 4.06 mg/kg. Pellets and powder had higher mycotoxin contamination compared to other commercially available fish feed types. This study shows AFB1 as a potential fish feed safety issue in the LVB and suggests a need for more research on mycotoxin residues in fish fillets. |
| format | Journal Article |
| id | CGSpace110624 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1106242024-05-01T08:18:44Z Assessment of fungal contamination in fish feed from the Lake Victoria Basin, Uganda Namulawa, V.T. Mutiga, Samuel K. Musimbi, F. Akello, S. Ng'ang'a, Fredrick M. Kago, Leah Kyallo, Martina M. Harvey, Jagger J.W. Ghimire, Sita R. fish feeds biological contamination fish feeding lake victoria The emergence of commercial fish farming has stimulated the establishment of fish feed factories in Uganda. However, no information is available on the safety of the feed, mainly due to lack of mycotoxin testing facilities and weak regulatory systems. A study was carried out to examine fungal colonization and mycotoxin contamination in fish feed samples (n = 147) of different types collected from nine fish farms (n = 81) and seven fish feed factories (n = 66) in the Lake Victoria Basin (LVB). Fungi were isolated in potato dextrose agar, grouped into morphotypes and representative isolates from each morphotype were identified based on the internal transcribed spacer (ITS) region of ribosomal DNA sequences. Aflatoxin B1 (AFB1) and total fumonisin (combinations of B1, B2 and B3; hereinafter named fumonisin) levels in feed samples were determined by enzyme-linked immunosorbent assay (ELISA). A wide range of fungi, including toxigenic Aspergillus flavus and Fusarium verticillioides, were isolated from the fish feed samples. AFB1 was detected in 48% of the factory samples and in 63% of the farm samples, with toxin levels <40 and >400 µg/kg, respectively. Similarly, 31% of the factory samples and 29% of the farm samples had fumonisin contamination ranging between 0.1 and 4.06 mg/kg. Pellets and powder had higher mycotoxin contamination compared to other commercially available fish feed types. This study shows AFB1 as a potential fish feed safety issue in the LVB and suggests a need for more research on mycotoxin residues in fish fillets. 2020-04-07 2020-12-24T09:04:29Z 2020-12-24T09:04:29Z Journal Article https://hdl.handle.net/10568/110624 en Open Access MDPI Namulawa, V.T., Mutiga, S., Musimbi, F., Akello, S., Ng'ang'a, F., Kago, L., Kyallo, M., Harvey, J. and Ghimire, S. 2020. Assessment of fungal contamination in fish feed from the Lake Victoria Basin, Uganda. Toxins 12:233. |
| spellingShingle | fish feeds biological contamination fish feeding lake victoria Namulawa, V.T. Mutiga, Samuel K. Musimbi, F. Akello, S. Ng'ang'a, Fredrick M. Kago, Leah Kyallo, Martina M. Harvey, Jagger J.W. Ghimire, Sita R. Assessment of fungal contamination in fish feed from the Lake Victoria Basin, Uganda |
| title | Assessment of fungal contamination in fish feed from the Lake Victoria Basin, Uganda |
| title_full | Assessment of fungal contamination in fish feed from the Lake Victoria Basin, Uganda |
| title_fullStr | Assessment of fungal contamination in fish feed from the Lake Victoria Basin, Uganda |
| title_full_unstemmed | Assessment of fungal contamination in fish feed from the Lake Victoria Basin, Uganda |
| title_short | Assessment of fungal contamination in fish feed from the Lake Victoria Basin, Uganda |
| title_sort | assessment of fungal contamination in fish feed from the lake victoria basin uganda |
| topic | fish feeds biological contamination fish feeding lake victoria |
| url | https://hdl.handle.net/10568/110624 |
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