Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 protein
The CD4 protein is an important surface marker of T lymphocytes, which can mediate the antigen presentation process by interacting with MHC II and TCR molecules in human and mouse.In this study, two haplotypes (A and B) of the CD4 gene were found within Chinese indigenous and Western commercial pig...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/110499 |
| _version_ | 1855528524360712192 |
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| author | Zhang, Weiya Ni, Juan Zhang, Jie Zhang, Lu Zhou, Huanhuan Zhao, Changzhi Zhu, Mengjin Wang, Haiyan Han Jianlin Li, Xinyun Zhao, Shuhong |
| author_browse | Han Jianlin Li, Xinyun Ni, Juan Wang, Haiyan Zhang, Jie Zhang, Lu Zhang, Weiya Zhao, Changzhi Zhao, Shuhong Zhou, Huanhuan Zhu, Mengjin |
| author_facet | Zhang, Weiya Ni, Juan Zhang, Jie Zhang, Lu Zhou, Huanhuan Zhao, Changzhi Zhu, Mengjin Wang, Haiyan Han Jianlin Li, Xinyun Zhao, Shuhong |
| author_sort | Zhang, Weiya |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The CD4 protein is an important surface marker of T lymphocytes, which can mediate the antigen presentation process by interacting with MHC II and TCR molecules in human and mouse.In this study, two haplotypes (A and B) of the CD4 gene were found within Chinese indigenous and Western commercial pig breeds. These two haplotypes were defined by 22 fully linked SNPs in the CDS region of the CD4 gene. The expression level and localization of the CD4 protein were significantly different between haplotypes A and B. Transcriptome analysis revealed that the immune response-related genes and signaling pathways were down-regulated in genotype AA. Finally, three linked functional SNPs were identified, which affected the expression level and membrane localization of the CD4 protein in pigs. These three SNPs led to the replacements of two amino acids in the IgV1 domain of the CD4 protein, and related to the function of the CD4 protein in the immune response.These three linked SNPs were the key functional mutation sites in the CD4 gene, which played important roles in the immune response, and could be utilized as new molecular markers in breeding for disease resistance in pigs. |
| format | Journal Article |
| id | CGSpace110499 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1104992024-05-01T08:17:54Z Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 protein Zhang, Weiya Ni, Juan Zhang, Jie Zhang, Lu Zhou, Huanhuan Zhao, Changzhi Zhu, Mengjin Wang, Haiyan Han Jianlin Li, Xinyun Zhao, Shuhong swine animal breeding genetics The CD4 protein is an important surface marker of T lymphocytes, which can mediate the antigen presentation process by interacting with MHC II and TCR molecules in human and mouse.In this study, two haplotypes (A and B) of the CD4 gene were found within Chinese indigenous and Western commercial pig breeds. These two haplotypes were defined by 22 fully linked SNPs in the CDS region of the CD4 gene. The expression level and localization of the CD4 protein were significantly different between haplotypes A and B. Transcriptome analysis revealed that the immune response-related genes and signaling pathways were down-regulated in genotype AA. Finally, three linked functional SNPs were identified, which affected the expression level and membrane localization of the CD4 protein in pigs. These three SNPs led to the replacements of two amino acids in the IgV1 domain of the CD4 protein, and related to the function of the CD4 protein in the immune response.These three linked SNPs were the key functional mutation sites in the CD4 gene, which played important roles in the immune response, and could be utilized as new molecular markers in breeding for disease resistance in pigs. 2020-12 2020-12-14T10:59:19Z 2020-12-14T10:59:19Z Journal Article https://hdl.handle.net/10568/110499 en Open Access Springer Zhang, Weiya, Ni, Juan, Zhang, Jie, Zhang, Lu, Zhou, Huanhuan, Zhao, Changzhi, Zhu, Mengjin, Wang, Haiyan, Han, Jianlin, Li, Xinyun and Zhao, Shuhong. 2020. Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 protein. BMC Molecular and Cell Biology 21:91. |
| spellingShingle | swine animal breeding genetics Zhang, Weiya Ni, Juan Zhang, Jie Zhang, Lu Zhou, Huanhuan Zhao, Changzhi Zhu, Mengjin Wang, Haiyan Han Jianlin Li, Xinyun Zhao, Shuhong Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 protein |
| title | Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 protein |
| title_full | Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 protein |
| title_fullStr | Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 protein |
| title_full_unstemmed | Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 protein |
| title_short | Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 protein |
| title_sort | three functional mutation sites affect the immune response of pigs through altering the expression pattern and igv domain of the cd4 protein |
| topic | swine animal breeding genetics |
| url | https://hdl.handle.net/10568/110499 |
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