A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses
Toxic concentrations of aluminium cations and low phosphorus availability are the main yield-limiting factors in acidic soils, which represent half of the potentially available arable land. Brachiaria grasses, which are commonly sown as forage in the tropics because of their resilience and low deman...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Oxford University Press
2021
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/110412 |
| _version_ | 1855537705433169920 |
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| author | Worthington, Margaret Pérez, Juan Guillermo Mussurova, Saule Silva Córdoba, Alexander Castiblanco, Valheria Cardoso Arango, Juan Andrés Jones, Charlotte Fernandez-Fuentes, Narcis Skot, Leif Dyer, Sarah Tohme, Joseph M. Palma, Federica di Arango, Jacobo Armstead, Ian Vega, Jose J. de |
| author_browse | Arango, Jacobo Armstead, Ian Cardoso Arango, Juan Andrés Castiblanco, Valheria Dyer, Sarah Fernandez-Fuentes, Narcis Jones, Charlotte Mussurova, Saule Palma, Federica di Pérez, Juan Guillermo Silva Córdoba, Alexander Skot, Leif Tohme, Joseph M. Vega, Jose J. de Worthington, Margaret |
| author_facet | Worthington, Margaret Pérez, Juan Guillermo Mussurova, Saule Silva Córdoba, Alexander Castiblanco, Valheria Cardoso Arango, Juan Andrés Jones, Charlotte Fernandez-Fuentes, Narcis Skot, Leif Dyer, Sarah Tohme, Joseph M. Palma, Federica di Arango, Jacobo Armstead, Ian Vega, Jose J. de |
| author_sort | Worthington, Margaret |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Toxic concentrations of aluminium cations and low phosphorus availability are the main yield-limiting factors in acidic
soils, which represent half of the potentially available arable land. Brachiaria grasses, which are commonly sown as
forage in the tropics because of their resilience and low demand for nutrients, show greater tolerance to high concentrations
of aluminium cations (Al3+) than most other grass crops. In this work, we explored the natural variation
in tolerance to Al3+ between high and low tolerant Brachiaria species and characterized their transcriptional differences
during stress. We identified three QTLs (quantitative trait loci) associated with root vigour during Al3+ stress
in their hybrid progeny. By integrating these results with a new Brachiaria reference genome, we identified 30 genes
putatively responsible for Al3+ tolerance in Brachiaria. We observed differential expression during stress of genes involved
in RNA translation, response signalling, cell wall composition, and vesicle location homologous to aluminiuminduced
proteins involved in limiting uptake or localizing the toxin. However, there was limited regulation of malate
transporters in Brachiaria, which suggests that exudation of organic acids and other external tolerance mechanisms,
common in other grasses, might not be relevant in Brachiaria. The contrasting regulation of RNA translation and response
signalling suggests that response timing is critical in high Al3+-tolerant Brachiaria. |
| format | Journal Article |
| id | CGSpace110412 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1104122025-11-11T19:04:20Z A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses Worthington, Margaret Pérez, Juan Guillermo Mussurova, Saule Silva Córdoba, Alexander Castiblanco, Valheria Cardoso Arango, Juan Andrés Jones, Charlotte Fernandez-Fuentes, Narcis Skot, Leif Dyer, Sarah Tohme, Joseph M. Palma, Federica di Arango, Jacobo Armstead, Ian Vega, Jose J. de acid soils suelo ácido brachiaria urochloa quantitative trait loci aluminium genomes Toxic concentrations of aluminium cations and low phosphorus availability are the main yield-limiting factors in acidic soils, which represent half of the potentially available arable land. Brachiaria grasses, which are commonly sown as forage in the tropics because of their resilience and low demand for nutrients, show greater tolerance to high concentrations of aluminium cations (Al3+) than most other grass crops. In this work, we explored the natural variation in tolerance to Al3+ between high and low tolerant Brachiaria species and characterized their transcriptional differences during stress. We identified three QTLs (quantitative trait loci) associated with root vigour during Al3+ stress in their hybrid progeny. By integrating these results with a new Brachiaria reference genome, we identified 30 genes putatively responsible for Al3+ tolerance in Brachiaria. We observed differential expression during stress of genes involved in RNA translation, response signalling, cell wall composition, and vesicle location homologous to aluminiuminduced proteins involved in limiting uptake or localizing the toxin. However, there was limited regulation of malate transporters in Brachiaria, which suggests that exudation of organic acids and other external tolerance mechanisms, common in other grasses, might not be relevant in Brachiaria. The contrasting regulation of RNA translation and response signalling suggests that response timing is critical in high Al3+-tolerant Brachiaria. 2021-02-02 2020-12-04T22:14:34Z 2020-12-04T22:14:34Z Journal Article https://hdl.handle.net/10568/110412 en Open Access application/pdf Oxford University Press Worthington, M.; Perez, J.G.; Mussurova, S.; Silva-Cordoba, A.; Castiblanco, V.; Cardoso Arango, J.A.; Jones, C.; Fernandez-Fuentes, N.; Skot, L.; Dyer, S.; Tohme, J.; Di Palma, F.; Arango, J.; Armstead, I.; De Vega, J.J. (2020) A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses. Journal of Experimental Botany, Online first paper (16 October 2020). ISSN: 0022-0957. |
| spellingShingle | acid soils suelo ácido brachiaria urochloa quantitative trait loci aluminium genomes Worthington, Margaret Pérez, Juan Guillermo Mussurova, Saule Silva Córdoba, Alexander Castiblanco, Valheria Cardoso Arango, Juan Andrés Jones, Charlotte Fernandez-Fuentes, Narcis Skot, Leif Dyer, Sarah Tohme, Joseph M. Palma, Federica di Arango, Jacobo Armstead, Ian Vega, Jose J. de A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses |
| title | A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses |
| title_full | A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses |
| title_fullStr | A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses |
| title_full_unstemmed | A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses |
| title_short | A new genome allows the identification of genes associated with natural variation in aluminium tolerance in Brachiaria grasses |
| title_sort | new genome allows the identification of genes associated with natural variation in aluminium tolerance in brachiaria grasses |
| topic | acid soils suelo ácido brachiaria urochloa quantitative trait loci aluminium genomes |
| url | https://hdl.handle.net/10568/110412 |
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