The Cassava Source-Sink project: opportunities and challenges for crop improvement by metabolic engineering
Cassava (Manihot esculenta Crantz) is one of the important staple foods in Sub‐Saharan Africa. It produces starchy storage roots that provide food and income for several hundred million people, mainly in tropical agriculture zones. Increasing cassava storage root and starch yield is one of the major...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2020
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/110092 |
| _version_ | 1855539341997113344 |
|---|---|
| author | Sonnewald, U. Fernie, A.R. Gruissem, W. Schlapfer, P. Anjanappa, R.B. Chang, S.H. Ludewig, F. Rascher, U. Muller, O. Doorn, A.M. van Rabbi, Ismail Y. Zierer, W. |
| author_browse | Anjanappa, R.B. Chang, S.H. Doorn, A.M. van Fernie, A.R. Gruissem, W. Ludewig, F. Muller, O. Rabbi, Ismail Y. Rascher, U. Schlapfer, P. Sonnewald, U. Zierer, W. |
| author_facet | Sonnewald, U. Fernie, A.R. Gruissem, W. Schlapfer, P. Anjanappa, R.B. Chang, S.H. Ludewig, F. Rascher, U. Muller, O. Doorn, A.M. van Rabbi, Ismail Y. Zierer, W. |
| author_sort | Sonnewald, U. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava (Manihot esculenta Crantz) is one of the important staple foods in Sub‐Saharan Africa. It produces starchy storage roots that provide food and income for several hundred million people, mainly in tropical agriculture zones. Increasing cassava storage root and starch yield is one of the major breeding targets with respect to securing the future food supply for the growing population of Sub‐Saharan Africa. The Cassava Source–Sink (CASS) project aims to increase cassava storage root and starch yield by strategically integrating approaches from different disciplines. We present our perspective and progress on cassava as an applied research organism and provide insight into the CASS strategy, which can serve as a blueprint for the improvement of other root and tuber crops. Extensive profiling of different field‐grown cassava genotypes generates information for leaf, phloem, and root metabolic and physiological processes that are relevant for biotechnological improvements. A multi‐national pipeline for genetic engineering of cassava plants covers all steps from gene discovery, cloning, transformation, molecular and biochemical characterization, confined field trials, and phenotyping of the seasonal dynamics of shoot traits under field conditions. Together, the CASS project generates comprehensive data to facilitate conventional breeding strategies for high‐yielding cassava genotypes. It also builds the foundation for genome‐scale metabolic modelling aiming to predict targets and bottlenecks in metabolic pathways. This information is used to engineer cassava genotypes with improved source–sink relations and increased yield potential. |
| format | Journal Article |
| id | CGSpace110092 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1100922025-12-08T09:54:28Z The Cassava Source-Sink project: opportunities and challenges for crop improvement by metabolic engineering Sonnewald, U. Fernie, A.R. Gruissem, W. Schlapfer, P. Anjanappa, R.B. Chang, S.H. Ludewig, F. Rascher, U. Muller, O. Doorn, A.M. van Rabbi, Ismail Y. Zierer, W. cassava manihot esculenta biotechnology yields starch subsaharan africa crop improvement genotypes genetic variation Cassava (Manihot esculenta Crantz) is one of the important staple foods in Sub‐Saharan Africa. It produces starchy storage roots that provide food and income for several hundred million people, mainly in tropical agriculture zones. Increasing cassava storage root and starch yield is one of the major breeding targets with respect to securing the future food supply for the growing population of Sub‐Saharan Africa. The Cassava Source–Sink (CASS) project aims to increase cassava storage root and starch yield by strategically integrating approaches from different disciplines. We present our perspective and progress on cassava as an applied research organism and provide insight into the CASS strategy, which can serve as a blueprint for the improvement of other root and tuber crops. Extensive profiling of different field‐grown cassava genotypes generates information for leaf, phloem, and root metabolic and physiological processes that are relevant for biotechnological improvements. A multi‐national pipeline for genetic engineering of cassava plants covers all steps from gene discovery, cloning, transformation, molecular and biochemical characterization, confined field trials, and phenotyping of the seasonal dynamics of shoot traits under field conditions. Together, the CASS project generates comprehensive data to facilitate conventional breeding strategies for high‐yielding cassava genotypes. It also builds the foundation for genome‐scale metabolic modelling aiming to predict targets and bottlenecks in metabolic pathways. This information is used to engineer cassava genotypes with improved source–sink relations and increased yield potential. 2020-06-05 2020-11-09T15:13:35Z 2020-11-09T15:13:35Z Journal Article https://hdl.handle.net/10568/110092 en Open Access application/pdf Wiley Sonnewald, U., Fernie, A.R., Gruissem, W., Schläpfer, P., Anjanappa, R.B., Chang, S.H., ... & Zierer, W. (2020). The Cassava Source–Sink project: opportunities and challenges for crop improvement by metabolic engineering. The Plant Journal, 103(5), 1655-1665. |
| spellingShingle | cassava manihot esculenta biotechnology yields starch subsaharan africa crop improvement genotypes genetic variation Sonnewald, U. Fernie, A.R. Gruissem, W. Schlapfer, P. Anjanappa, R.B. Chang, S.H. Ludewig, F. Rascher, U. Muller, O. Doorn, A.M. van Rabbi, Ismail Y. Zierer, W. The Cassava Source-Sink project: opportunities and challenges for crop improvement by metabolic engineering |
| title | The Cassava Source-Sink project: opportunities and challenges for crop improvement by metabolic engineering |
| title_full | The Cassava Source-Sink project: opportunities and challenges for crop improvement by metabolic engineering |
| title_fullStr | The Cassava Source-Sink project: opportunities and challenges for crop improvement by metabolic engineering |
| title_full_unstemmed | The Cassava Source-Sink project: opportunities and challenges for crop improvement by metabolic engineering |
| title_short | The Cassava Source-Sink project: opportunities and challenges for crop improvement by metabolic engineering |
| title_sort | cassava source sink project opportunities and challenges for crop improvement by metabolic engineering |
| topic | cassava manihot esculenta biotechnology yields starch subsaharan africa crop improvement genotypes genetic variation |
| url | https://hdl.handle.net/10568/110092 |
| work_keys_str_mv | AT sonnewaldu thecassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT ferniear thecassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT gruissemw thecassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT schlapferp thecassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT anjanapparb thecassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT changsh thecassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT ludewigf thecassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT rascheru thecassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT mullero thecassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT doornamvan thecassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT rabbiismaily thecassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT ziererw thecassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT sonnewaldu cassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT ferniear cassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT gruissemw cassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT schlapferp cassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT anjanapparb cassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT changsh cassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT ludewigf cassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT rascheru cassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT mullero cassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT doornamvan cassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT rabbiismaily cassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering AT ziererw cassavasourcesinkprojectopportunitiesandchallengesforcropimprovementbymetabolicengineering |