Challenges for ex situ conservation of wild bananas: seeds collected in Papua New Guinea have variable levels of desiccation tolerance
Ex situ seed conservation of banana crop wild relatives (Musa spp. L.), is constrained by critical knowledge gaps in their storage and germination behaviour. Additionally, challenges in collecting seeds from wild populations impact the quality of seed collections. It is, therefore, crucial to evalua...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2020
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/109939 |
| _version_ | 1855530682618478592 |
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| author | Kallow, Simon Longin, Kevin Fanega Sleziak, Natalia Janssens, Steven B. Vandelook, Filip Dickie, John Swennen, Rony L. Paofa, Janet Carpentier, Sebastien C. Panis, Bartholomeus |
| author_browse | Carpentier, Sebastien C. Dickie, John Fanega Sleziak, Natalia Janssens, Steven B. Kallow, Simon Longin, Kevin Panis, Bartholomeus Paofa, Janet Swennen, Rony L. Vandelook, Filip |
| author_facet | Kallow, Simon Longin, Kevin Fanega Sleziak, Natalia Janssens, Steven B. Vandelook, Filip Dickie, John Swennen, Rony L. Paofa, Janet Carpentier, Sebastien C. Panis, Bartholomeus |
| author_sort | Kallow, Simon |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Ex situ seed conservation of banana crop wild relatives (Musa spp. L.), is constrained by critical knowledge gaps in their storage and germination behaviour. Additionally, challenges in collecting seeds from wild populations impact the quality of seed collections. It is, therefore, crucial to evaluate the viability of seeds from such collecting missions in order to improve the value
of future seed collections. We evaluate the seed viability of 37 accessions of seven Musa species, collected from wild populations in Papua New Guinea, during two collecting missions. Seeds from one mission had already been stored in conventional storage (dried for four months at 15% relative humidity, 20 ◦C and stored for two months at 15% relative humdity, −20 ◦C), so a post-storage test was carried out. Seeds from the second mission were assessed freshly extracted and following desiccation.
We used embryo rescue techniques to overcome the barrier of germinating in vivo Musa seeds. Seeds from the first mission had low viability (19 ± 27% mean and standard deviation) after storage for two months at 15% relative humidity and −20 ◦C. Musa balbisiana Colla seeds had significantly higher post-storage germination than other species (p < 0.01). Desiccation reduced germination of the seeds from the second collecting mission, from 84 ± 22% (at 16.7 ± 2.4% moisture content) to 36 ± 30% (at 2.4 ± 0.8% moisture content). There was considerable variation between and (to a lesser extent) within accessions, a proportion of individual seeds of all but one species (Musa ingens N.W.Simmonds) survived desiccation and sub-zero temperature storage. We identified that seeds from the basal end of the infructescence were less likely to be viable after storage (p < 0.001); and made
morphological observations that identify seeds and infructescences with higher viability in relation to their developmental maturity. We highlight the need for research into seed eco-physiology of crop wild relatives in order to improve future collecting missions. |
| format | Journal Article |
| id | CGSpace109939 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1099392025-11-12T05:56:56Z Challenges for ex situ conservation of wild bananas: seeds collected in Papua New Guinea have variable levels of desiccation tolerance Kallow, Simon Longin, Kevin Fanega Sleziak, Natalia Janssens, Steven B. Vandelook, Filip Dickie, John Swennen, Rony L. Paofa, Janet Carpentier, Sebastien C. Panis, Bartholomeus banana crop wild relatives ex-situ conservation drying plant genetic resources seed storage banano especies silvestres afín a las plantas cultivadas conservación ex-situ secado Ex situ seed conservation of banana crop wild relatives (Musa spp. L.), is constrained by critical knowledge gaps in their storage and germination behaviour. Additionally, challenges in collecting seeds from wild populations impact the quality of seed collections. It is, therefore, crucial to evaluate the viability of seeds from such collecting missions in order to improve the value of future seed collections. We evaluate the seed viability of 37 accessions of seven Musa species, collected from wild populations in Papua New Guinea, during two collecting missions. Seeds from one mission had already been stored in conventional storage (dried for four months at 15% relative humidity, 20 ◦C and stored for two months at 15% relative humdity, −20 ◦C), so a post-storage test was carried out. Seeds from the second mission were assessed freshly extracted and following desiccation. We used embryo rescue techniques to overcome the barrier of germinating in vivo Musa seeds. Seeds from the first mission had low viability (19 ± 27% mean and standard deviation) after storage for two months at 15% relative humidity and −20 ◦C. Musa balbisiana Colla seeds had significantly higher post-storage germination than other species (p < 0.01). Desiccation reduced germination of the seeds from the second collecting mission, from 84 ± 22% (at 16.7 ± 2.4% moisture content) to 36 ± 30% (at 2.4 ± 0.8% moisture content). There was considerable variation between and (to a lesser extent) within accessions, a proportion of individual seeds of all but one species (Musa ingens N.W.Simmonds) survived desiccation and sub-zero temperature storage. We identified that seeds from the basal end of the infructescence were less likely to be viable after storage (p < 0.001); and made morphological observations that identify seeds and infructescences with higher viability in relation to their developmental maturity. We highlight the need for research into seed eco-physiology of crop wild relatives in order to improve future collecting missions. 2020-10 2020-10-22T14:41:43Z 2020-10-22T14:41:43Z Journal Article https://hdl.handle.net/10568/109939 en Open Access application/pdf MDPI Kallow, S.; Longin, K.; Fanega Sleziak, N.; Janssens, S.B.; Vandelook, F.; Dickie, J.; Swennen, R.; Paofa, J.; Carpentier, S.; Panis, B. (2020) Challenges for ex situ conservation of wild bananas: seeds collected in Papua New Guinea have variable levels of desiccation tolerance. Plants 9(9) 1243 ISSN: 2223-7747 |
| spellingShingle | banana crop wild relatives ex-situ conservation drying plant genetic resources seed storage banano especies silvestres afín a las plantas cultivadas conservación ex-situ secado Kallow, Simon Longin, Kevin Fanega Sleziak, Natalia Janssens, Steven B. Vandelook, Filip Dickie, John Swennen, Rony L. Paofa, Janet Carpentier, Sebastien C. Panis, Bartholomeus Challenges for ex situ conservation of wild bananas: seeds collected in Papua New Guinea have variable levels of desiccation tolerance |
| title | Challenges for ex situ conservation of wild bananas: seeds collected in Papua New Guinea have variable levels of desiccation tolerance |
| title_full | Challenges for ex situ conservation of wild bananas: seeds collected in Papua New Guinea have variable levels of desiccation tolerance |
| title_fullStr | Challenges for ex situ conservation of wild bananas: seeds collected in Papua New Guinea have variable levels of desiccation tolerance |
| title_full_unstemmed | Challenges for ex situ conservation of wild bananas: seeds collected in Papua New Guinea have variable levels of desiccation tolerance |
| title_short | Challenges for ex situ conservation of wild bananas: seeds collected in Papua New Guinea have variable levels of desiccation tolerance |
| title_sort | challenges for ex situ conservation of wild bananas seeds collected in papua new guinea have variable levels of desiccation tolerance |
| topic | banana crop wild relatives ex-situ conservation drying plant genetic resources seed storage banano especies silvestres afín a las plantas cultivadas conservación ex-situ secado |
| url | https://hdl.handle.net/10568/109939 |
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