CRISPR-Cas9-based genome editing of banana
Genome editing is an emerging powerful new breeding tool, which can be applied for genetic improvement of banana for important agronomic traits such as resistance to biotic stresses, adaptation to climate change, and high yielding. Banana is an important staple food and cash crop, feeding millions o...
| Autores principales: | , , |
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/109895 |
| _version_ | 1855518412127600640 |
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| author | Tripathi, L. Ntui, V.O. Tripathi, J.N. |
| author_browse | Ntui, V.O. Tripathi, J.N. Tripathi, L. |
| author_facet | Tripathi, L. Ntui, V.O. Tripathi, J.N. |
| author_sort | Tripathi, L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Genome editing is an emerging powerful new breeding tool, which can be applied for genetic improvement of banana for important agronomic traits such as resistance to biotic stresses, adaptation to climate change, and high yielding. Banana is an important staple food and cash crop, feeding millions of people in tropical and subtropical countries. Recently, CRISPR-Cas9-based genome editing system has been established for banana in a few laboratories. Here, we describe the procedures for generation of genome-edited events of banana, detection of targeted and potential off-target mutations, and phenotyping for important traits such as disease resistance. This chapter will provide readers strategy for applying CRISPR-Cas9-based genome editing for improvement of banana. |
| format | Book Chapter |
| id | CGSpace109895 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1098952023-12-08T19:36:04Z CRISPR-Cas9-based genome editing of banana Tripathi, L. Ntui, V.O. Tripathi, J.N. bananas genomes breeding disease resistance east africa agronomic characters genetic improvement Genome editing is an emerging powerful new breeding tool, which can be applied for genetic improvement of banana for important agronomic traits such as resistance to biotic stresses, adaptation to climate change, and high yielding. Banana is an important staple food and cash crop, feeding millions of people in tropical and subtropical countries. Recently, CRISPR-Cas9-based genome editing system has been established for banana in a few laboratories. Here, we describe the procedures for generation of genome-edited events of banana, detection of targeted and potential off-target mutations, and phenotyping for important traits such as disease resistance. This chapter will provide readers strategy for applying CRISPR-Cas9-based genome editing for improvement of banana. 2020 2020-10-19T10:25:42Z 2020-10-19T10:25:42Z Book Chapter https://hdl.handle.net/10568/109895 en Limited Access Springer Tripathi, L., Ntui, V.O. & Tripathi, J.N. (2020). CRISPR-Cas9-based genome editing of banana. In M.T. Islam, P.K. Bhowmik and K.A. Molla, CRISPR-Cas methods. New York, United States: Springer. (p. 223-236). |
| spellingShingle | bananas genomes breeding disease resistance east africa agronomic characters genetic improvement Tripathi, L. Ntui, V.O. Tripathi, J.N. CRISPR-Cas9-based genome editing of banana |
| title | CRISPR-Cas9-based genome editing of banana |
| title_full | CRISPR-Cas9-based genome editing of banana |
| title_fullStr | CRISPR-Cas9-based genome editing of banana |
| title_full_unstemmed | CRISPR-Cas9-based genome editing of banana |
| title_short | CRISPR-Cas9-based genome editing of banana |
| title_sort | crispr cas9 based genome editing of banana |
| topic | bananas genomes breeding disease resistance east africa agronomic characters genetic improvement |
| url | https://hdl.handle.net/10568/109895 |
| work_keys_str_mv | AT tripathil crisprcas9basedgenomeeditingofbanana AT ntuivo crisprcas9basedgenomeeditingofbanana AT tripathijn crisprcas9basedgenomeeditingofbanana |