Salix dormant bud cryotolerance varies by taxon, harvest year, and stem‐segment length
Willow (Salix L.) species have good qualities for becoming a biomass crop for production of biofuels. They grow on marginal soils, produce high yields of wood in a short period of time, and are easily propagated. Salix is one of the few genera that may be cryopreserved as dormant winter buds (DBs) i...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Wiley
2020
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/109765 |
| _version_ | 1855524307680100352 |
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| author | Jenderek, M.M. Ambruzs, B. Holman, G.E. Carstens, J.D. Ellis, David Widrlechner, Mark P. |
| author_browse | Ambruzs, B. Carstens, J.D. Ellis, David Holman, G.E. Jenderek, M.M. Widrlechner, Mark P. |
| author_facet | Jenderek, M.M. Ambruzs, B. Holman, G.E. Carstens, J.D. Ellis, David Widrlechner, Mark P. |
| author_sort | Jenderek, M.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Willow (Salix L.) species have good qualities for becoming a biomass crop for production of biofuels. They grow on marginal soils, produce high yields of wood in a short period of time, and are easily propagated. Salix is one of the few genera that may be cryopreserved as dormant winter buds (DBs) instead of using tissue culture techniques. The objective of our study was to evaluate selected factors that affect cryotolerance of nine Salix germplasm accessions in the USDA‐ARS National Plant Germplasm System collection. One‐year‐old branches harvested in January over 3 yr were cut into either 6‐ to 7‐ or 10‐cm segments containing at least two DBs. Segments were treated with a slow‐cooling procedure and stored in vapor‐phase liquid N (LNV). Control segments were stored at −5 °C until planting. The LNV‐exposed and the −5 °C‐stored nodal segments were warmed and directly planted in a cold greenhouse. Six weeks later, the material was evaluated for shoot and root development. Segments that developed a shoot were considered viable. Average viability varied among years (0–35.1%) even for the same accession, species (4.2–51.4%), and segment length. Dormant buds on 10‐cm segments had higher average viability (82.2%) than did DBs on 6‐ to 7‐cm segments (43.9%), suggesting higher suitability for cryopreservation. In the material studied, Salix DB viability was correlated with branch diameter, DB density, and the ability to develop shoots and roots prior to cryopreservation. |
| format | Journal Article |
| id | CGSpace109765 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1097652025-11-29T05:22:15Z Salix dormant bud cryotolerance varies by taxon, harvest year, and stem‐segment length Jenderek, M.M. Ambruzs, B. Holman, G.E. Carstens, J.D. Ellis, David Widrlechner, Mark P. salix dormancy cryopreservation biomass Willow (Salix L.) species have good qualities for becoming a biomass crop for production of biofuels. They grow on marginal soils, produce high yields of wood in a short period of time, and are easily propagated. Salix is one of the few genera that may be cryopreserved as dormant winter buds (DBs) instead of using tissue culture techniques. The objective of our study was to evaluate selected factors that affect cryotolerance of nine Salix germplasm accessions in the USDA‐ARS National Plant Germplasm System collection. One‐year‐old branches harvested in January over 3 yr were cut into either 6‐ to 7‐ or 10‐cm segments containing at least two DBs. Segments were treated with a slow‐cooling procedure and stored in vapor‐phase liquid N (LNV). Control segments were stored at −5 °C until planting. The LNV‐exposed and the −5 °C‐stored nodal segments were warmed and directly planted in a cold greenhouse. Six weeks later, the material was evaluated for shoot and root development. Segments that developed a shoot were considered viable. Average viability varied among years (0–35.1%) even for the same accession, species (4.2–51.4%), and segment length. Dormant buds on 10‐cm segments had higher average viability (82.2%) than did DBs on 6‐ to 7‐cm segments (43.9%), suggesting higher suitability for cryopreservation. In the material studied, Salix DB viability was correlated with branch diameter, DB density, and the ability to develop shoots and roots prior to cryopreservation. 2020-07 2020-10-07T18:37:21Z 2020-10-07T18:37:21Z Journal Article https://hdl.handle.net/10568/109765 en Limited Access Wiley Jenderek, M.M.; Ambruzs, B.; Holman, G.E.; Carstens, J.D.; Ellis, D.; Widrlechner, M.P. (2020). Salix dormant bud cryotolerance varies by taxon, harvest year, and stem‐segment length. Crop Science. ISSN 1435-0653. 60:4 pp. 1965-1973 |
| spellingShingle | salix dormancy cryopreservation biomass Jenderek, M.M. Ambruzs, B. Holman, G.E. Carstens, J.D. Ellis, David Widrlechner, Mark P. Salix dormant bud cryotolerance varies by taxon, harvest year, and stem‐segment length |
| title | Salix dormant bud cryotolerance varies by taxon, harvest year, and stem‐segment length |
| title_full | Salix dormant bud cryotolerance varies by taxon, harvest year, and stem‐segment length |
| title_fullStr | Salix dormant bud cryotolerance varies by taxon, harvest year, and stem‐segment length |
| title_full_unstemmed | Salix dormant bud cryotolerance varies by taxon, harvest year, and stem‐segment length |
| title_short | Salix dormant bud cryotolerance varies by taxon, harvest year, and stem‐segment length |
| title_sort | salix dormant bud cryotolerance varies by taxon harvest year and stem segment length |
| topic | salix dormancy cryopreservation biomass |
| url | https://hdl.handle.net/10568/109765 |
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