Detection of Brucella spp. in raw milk from various livestock species raised under pastoral production systems in Isiolo and Marsabit Counties, northern Kenya
Introduction Brucellosis is an important zoonotic disease in Kenya, and identifying the bacteria in milk is important in assessing the risk of exposure in people. Methods A cross-sectional study that involved 175 households was implemented in the pastoral counties of Marsabit and Isiolo in Kenya. Po...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Springer
2020
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| Acceso en línea: | https://hdl.handle.net/10568/109553 |
| _version_ | 1855531664269115392 |
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| author | Wainaina, Martin Aboge, G.O. Omwenga, I. Ngaywa, C. Ngwili, Nicholas M. Kiara, Henry K. Wamwere-Njoroge, George J. Bett, Bernard K. |
| author_browse | Aboge, G.O. Bett, Bernard K. Kiara, Henry K. Ngaywa, C. Ngwili, Nicholas M. Omwenga, I. Wainaina, Martin Wamwere-Njoroge, George J. |
| author_facet | Wainaina, Martin Aboge, G.O. Omwenga, I. Ngaywa, C. Ngwili, Nicholas M. Kiara, Henry K. Wamwere-Njoroge, George J. Bett, Bernard K. |
| author_sort | Wainaina, Martin |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Introduction Brucellosis is an important zoonotic disease in Kenya, and identifying the bacteria in milk is important in assessing the risk of exposure in people. Methods A cross-sectional study that involved 175 households was implemented in the pastoral counties of Marsabit and Isiolo in Kenya. Pooled milk samples (n = 164) were collected at the household level, and another 372 were collected from domesticated lactating animals (312 goats, 7 sheep, 50 cattle and 3 camels). Real-time polymerase chain reaction (qPCR) testing of the milk samples was performed to identify Brucella species. Brucella anti-LPS IgG antibodies were also detected in bovine milk samples using an indirect enzyme-linked immunosorbent assay (ELISA). Results Based on the qPCR, the prevalence of the pathogen at the animal level (considering samples from individual animals) was 2.4% (95% confidence interval (CI) 1.1–4.5) and 3.0% (CI: 1.0–7.0) in pooled samples. All 14 samples found positive by qPCR were from goats, with 10 contaminated with B. abortus and 4 with B. melitensis. The Brucella spp. antibody prevalence in bovine milk using the milk ELISA was 26.0% (95% CI: 14.6–40.3) in individual animal samples and 46.3% (95% CI: 30.7–62.6) in pooled samples. Conclusion The study is the first in Kenya to test for Brucella spp. directly from milk using qPCR without culturing for the bacteria. It also detected B. abortus in goats, suggesting transmission of brucellosis between cattle and goats. The high prevalence of Brucella spp. is a significant public health risk, and there is a need for intervention strategies necessary in the study area. |
| format | Journal Article |
| id | CGSpace109553 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1095532024-05-01T08:18:22Z Detection of Brucella spp. in raw milk from various livestock species raised under pastoral production systems in Isiolo and Marsabit Counties, northern Kenya Wainaina, Martin Aboge, G.O. Omwenga, I. Ngaywa, C. Ngwili, Nicholas M. Kiara, Henry K. Wamwere-Njoroge, George J. Bett, Bernard K. brucella milk dairying livestock pastoralism zoonoses health Introduction Brucellosis is an important zoonotic disease in Kenya, and identifying the bacteria in milk is important in assessing the risk of exposure in people. Methods A cross-sectional study that involved 175 households was implemented in the pastoral counties of Marsabit and Isiolo in Kenya. Pooled milk samples (n = 164) were collected at the household level, and another 372 were collected from domesticated lactating animals (312 goats, 7 sheep, 50 cattle and 3 camels). Real-time polymerase chain reaction (qPCR) testing of the milk samples was performed to identify Brucella species. Brucella anti-LPS IgG antibodies were also detected in bovine milk samples using an indirect enzyme-linked immunosorbent assay (ELISA). Results Based on the qPCR, the prevalence of the pathogen at the animal level (considering samples from individual animals) was 2.4% (95% confidence interval (CI) 1.1–4.5) and 3.0% (CI: 1.0–7.0) in pooled samples. All 14 samples found positive by qPCR were from goats, with 10 contaminated with B. abortus and 4 with B. melitensis. The Brucella spp. antibody prevalence in bovine milk using the milk ELISA was 26.0% (95% CI: 14.6–40.3) in individual animal samples and 46.3% (95% CI: 30.7–62.6) in pooled samples. Conclusion The study is the first in Kenya to test for Brucella spp. directly from milk using qPCR without culturing for the bacteria. It also detected B. abortus in goats, suggesting transmission of brucellosis between cattle and goats. The high prevalence of Brucella spp. is a significant public health risk, and there is a need for intervention strategies necessary in the study area. 2020-11 2020-09-21T11:59:26Z 2020-09-21T11:59:26Z Journal Article https://hdl.handle.net/10568/109553 en Open Access Springer Wainaina, M., Aboge, G.O., Omwenga, I., Ngaywa, C., Ngwili, N., Kiara, H., Wamwere-Njoroge, G. and Bett, B. 2020. Detection of Brucella spp. in raw milk from various livestock species raised under pastoral production systems in Isiolo and Marsabit Counties, northern Kenya. Tropical Animal Health and Production 52: 3537–3544. |
| spellingShingle | brucella milk dairying livestock pastoralism zoonoses health Wainaina, Martin Aboge, G.O. Omwenga, I. Ngaywa, C. Ngwili, Nicholas M. Kiara, Henry K. Wamwere-Njoroge, George J. Bett, Bernard K. Detection of Brucella spp. in raw milk from various livestock species raised under pastoral production systems in Isiolo and Marsabit Counties, northern Kenya |
| title | Detection of Brucella spp. in raw milk from various livestock species raised under pastoral production systems in Isiolo and Marsabit Counties, northern Kenya |
| title_full | Detection of Brucella spp. in raw milk from various livestock species raised under pastoral production systems in Isiolo and Marsabit Counties, northern Kenya |
| title_fullStr | Detection of Brucella spp. in raw milk from various livestock species raised under pastoral production systems in Isiolo and Marsabit Counties, northern Kenya |
| title_full_unstemmed | Detection of Brucella spp. in raw milk from various livestock species raised under pastoral production systems in Isiolo and Marsabit Counties, northern Kenya |
| title_short | Detection of Brucella spp. in raw milk from various livestock species raised under pastoral production systems in Isiolo and Marsabit Counties, northern Kenya |
| title_sort | detection of brucella spp in raw milk from various livestock species raised under pastoral production systems in isiolo and marsabit counties northern kenya |
| topic | brucella milk dairying livestock pastoralism zoonoses health |
| url | https://hdl.handle.net/10568/109553 |
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