Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica)

Host-specific interactions can maintain genetic and phenotypic diversity in parasites that attack multiple host species. Host diversity, in turn, may promote parasite diversity by selection for genetic divergence or plastic responses to host type. The parasitic weed purple witchweed [Striga hermonth...

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Autores principales: López, L., Bellis, E.S., Wafula, E., Hearne, S., Honaas, L., Ralph, P.E., Timko, M.P., Unachukwu, N.N., dePamphilis, C.W., Lasky, J.R.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Cambridge University Press 2019
Materias:
Acceso en línea:https://hdl.handle.net/10568/109247
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author López, L.
Bellis, E.S.
Wafula, E.
Hearne, S.
Honaas, L.
Ralph, P.E.
Timko, M.P.
Unachukwu, N.N.
dePamphilis, C.W.
Lasky, J.R.
author_browse Bellis, E.S.
Hearne, S.
Honaas, L.
Lasky, J.R.
López, L.
Ralph, P.E.
Timko, M.P.
Unachukwu, N.N.
Wafula, E.
dePamphilis, C.W.
author_facet López, L.
Bellis, E.S.
Wafula, E.
Hearne, S.
Honaas, L.
Ralph, P.E.
Timko, M.P.
Unachukwu, N.N.
dePamphilis, C.W.
Lasky, J.R.
author_sort López, L.
collection Repository of Agricultural Research Outputs (CGSpace)
description Host-specific interactions can maintain genetic and phenotypic diversity in parasites that attack multiple host species. Host diversity, in turn, may promote parasite diversity by selection for genetic divergence or plastic responses to host type. The parasitic weed purple witchweed [Striga hermonthica (Delile) Benth.] causes devastating crop losses in sub-Saharan Africa and is capable of infesting a wide range of grass hosts. Despite some evidence for host adaptation and host-by-Striga genotype interactions, little is known about intraspecific Striga genomic diversity. Here we present a study of transcriptomic diversity in populations of S. hermonthica growing on different hosts (maize [Zea mays L.] vs. grain sorghum [Sorghum bicolor (L.) Moench]). We examined gene expression variation and differences in allelic frequency in expressed genes of aboveground tissues from populations in western Nigeria parasitizing each host. Despite low levels of host-based genome-wide differentiation, we identified a set of parasite transcripts specifically associated with each host. Parasite genes in several different functional categories implicated as important in host–parasite interactions differed in expression level and allele on different hosts, including genes involved in nutrient transport, defense and pathogenesis, and plant hormone response. Overall, we provide a set of candidate transcripts that demonstrate host-specific interactions in vegetative tissues of the emerged parasite S. hermonthica. Our study shows how signals of host-specific processes can be detected aboveground, expanding the focus of host–parasite interactions beyond the haustorial connection.
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spelling CGSpace1092472023-12-08T19:36:04Z Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica) López, L. Bellis, E.S. Wafula, E. Hearne, S. Honaas, L. Ralph, P.E. Timko, M.P. Unachukwu, N.N. dePamphilis, C.W. Lasky, J.R. allele frequency gene expression host parasite relations parasitic plant rna sequence maize striga hermonthica Host-specific interactions can maintain genetic and phenotypic diversity in parasites that attack multiple host species. Host diversity, in turn, may promote parasite diversity by selection for genetic divergence or plastic responses to host type. The parasitic weed purple witchweed [Striga hermonthica (Delile) Benth.] causes devastating crop losses in sub-Saharan Africa and is capable of infesting a wide range of grass hosts. Despite some evidence for host adaptation and host-by-Striga genotype interactions, little is known about intraspecific Striga genomic diversity. Here we present a study of transcriptomic diversity in populations of S. hermonthica growing on different hosts (maize [Zea mays L.] vs. grain sorghum [Sorghum bicolor (L.) Moench]). We examined gene expression variation and differences in allelic frequency in expressed genes of aboveground tissues from populations in western Nigeria parasitizing each host. Despite low levels of host-based genome-wide differentiation, we identified a set of parasite transcripts specifically associated with each host. Parasite genes in several different functional categories implicated as important in host–parasite interactions differed in expression level and allele on different hosts, including genes involved in nutrient transport, defense and pathogenesis, and plant hormone response. Overall, we provide a set of candidate transcripts that demonstrate host-specific interactions in vegetative tissues of the emerged parasite S. hermonthica. Our study shows how signals of host-specific processes can be detected aboveground, expanding the focus of host–parasite interactions beyond the haustorial connection. 2019-07 2020-09-07T11:31:32Z 2020-09-07T11:31:32Z Journal Article https://hdl.handle.net/10568/109247 en Limited Access Cambridge University Press Lopez, L., Bellis, E.S., Wafula, E., Hearne, S.J., Honaas, L., Ralph, P.E., ... & Lasky, J.R. (2019). Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica). Weed Science, 67(4), 397-411.
spellingShingle allele frequency
gene expression
host parasite relations
parasitic plant
rna sequence
maize
striga hermonthica
López, L.
Bellis, E.S.
Wafula, E.
Hearne, S.
Honaas, L.
Ralph, P.E.
Timko, M.P.
Unachukwu, N.N.
dePamphilis, C.W.
Lasky, J.R.
Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica)
title Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica)
title_full Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica)
title_fullStr Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica)
title_full_unstemmed Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica)
title_short Transcriptomics of host-specific interactions in natural populations of the parasitic plant purple witchweed (Striga hermonthica)
title_sort transcriptomics of host specific interactions in natural populations of the parasitic plant purple witchweed striga hermonthica
topic allele frequency
gene expression
host parasite relations
parasitic plant
rna sequence
maize
striga hermonthica
url https://hdl.handle.net/10568/109247
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