Bridging old and new: Diversity and evaluation of high iron-associated stress response of rice cultivated in West Africa

Adoption of rice varieties that perform well under high iron-associated (HIA) stress environments can enhance rice production in West Africa. This study reports the genetic characterization of 323 rice accessions and breeding lines cultivated in West Africa using genotyping-by-sequencing and their p...

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Main Authors: Diop, B., Wang, D.R., Drame, K.N., Gracen, V., Tongoona, P.B., Dzidzienyo, D., Nartey, E., Greenberg, A.J., Djiba, S., Danquah, E.Y., McCouch, S.R.
Format: Journal Article
Language:Inglés
Published: Oxford University Press 2020
Subjects:
Online Access:https://hdl.handle.net/10568/109110
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author Diop, B.
Wang, D.R.
Drame, K.N.
Gracen, V.
Tongoona, P.B.
Dzidzienyo, D.
Nartey, E.
Greenberg, A.J.
Djiba, S.
Danquah, E.Y.
McCouch, S.R.
author_browse Danquah, E.Y.
Diop, B.
Djiba, S.
Drame, K.N.
Dzidzienyo, D.
Gracen, V.
Greenberg, A.J.
McCouch, S.R.
Nartey, E.
Tongoona, P.B.
Wang, D.R.
author_facet Diop, B.
Wang, D.R.
Drame, K.N.
Gracen, V.
Tongoona, P.B.
Dzidzienyo, D.
Nartey, E.
Greenberg, A.J.
Djiba, S.
Danquah, E.Y.
McCouch, S.R.
author_sort Diop, B.
collection Repository of Agricultural Research Outputs (CGSpace)
description Adoption of rice varieties that perform well under high iron-associated (HIA) stress environments can enhance rice production in West Africa. This study reports the genetic characterization of 323 rice accessions and breeding lines cultivated in West Africa using genotyping-by-sequencing and their phenotypic response to HIA treatments in hydroponic solution (1500 mg l−1 FeSO4·7H2O) and hot-spot fields. The germplasm consisted of four genetic subpopulations: Oryza glaberrima (14%), O. sativa-japonica (7%), O. sativa-indica Group 1 (45%), and O. sativa-indica Group 2 (25%). Severe versus mild stress in the field was associated with a reduced SPAD value (12%), biomass (56%), and grain yield (57%), with leaf bronzing explaining 30% and 21% of the variation for biomass and grain yield, respectively. Association mapping using 175 indica genotypes identified 23 significant single nucleotide polymorphism (SNP) markers that mapped to 14 genomic regions. Genome-wide association study (GWAS) signals associated with leaf bronzing, a routinely used indicator of HIA stress, differed in hydroponic compared with field conditions. Contrastingly, six significant SNPs on chromosomes 8 and 9 were associated with the SPAD value under HIA stress in both field and hydroponic experiments, and a candidate potassium transporter gene mapped under the peak on chromosome 8. This study helps define criteria for assessing rice performance under HIA environments.
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spelling CGSpace1091102024-08-27T10:37:23Z Bridging old and new: Diversity and evaluation of high iron-associated stress response of rice cultivated in West Africa Diop, B. Wang, D.R. Drame, K.N. Gracen, V. Tongoona, P.B. Dzidzienyo, D. Nartey, E. Greenberg, A.J. Djiba, S. Danquah, E.Y. McCouch, S.R. rice genetic diversity research Adoption of rice varieties that perform well under high iron-associated (HIA) stress environments can enhance rice production in West Africa. This study reports the genetic characterization of 323 rice accessions and breeding lines cultivated in West Africa using genotyping-by-sequencing and their phenotypic response to HIA treatments in hydroponic solution (1500 mg l−1 FeSO4·7H2O) and hot-spot fields. The germplasm consisted of four genetic subpopulations: Oryza glaberrima (14%), O. sativa-japonica (7%), O. sativa-indica Group 1 (45%), and O. sativa-indica Group 2 (25%). Severe versus mild stress in the field was associated with a reduced SPAD value (12%), biomass (56%), and grain yield (57%), with leaf bronzing explaining 30% and 21% of the variation for biomass and grain yield, respectively. Association mapping using 175 indica genotypes identified 23 significant single nucleotide polymorphism (SNP) markers that mapped to 14 genomic regions. Genome-wide association study (GWAS) signals associated with leaf bronzing, a routinely used indicator of HIA stress, differed in hydroponic compared with field conditions. Contrastingly, six significant SNPs on chromosomes 8 and 9 were associated with the SPAD value under HIA stress in both field and hydroponic experiments, and a candidate potassium transporter gene mapped under the peak on chromosome 8. This study helps define criteria for assessing rice performance under HIA environments. 2020-07-06 2020-08-29T15:55:57Z 2020-08-29T15:55:57Z Journal Article https://hdl.handle.net/10568/109110 en Open Access Oxford University Press Diop, B., Wang, D.R., Drame, K.N., Gracen, V., Tongoona, P., Dzidzienyo, D., Nartey, E., Greenberg, A.J., Djiba, S., Danquah, E.Y., McCouch, S.R. Bridging old and new: Diversity and evaluation of high iron-associated stress response of rice cultivated in West Africa, Journal of Experimental Botany, Volume 71, Issue 14, 6 July 2020, Pages 4188–4200, https://doi.org/10.1093/jxb/eraa182
spellingShingle rice
genetic diversity
research
Diop, B.
Wang, D.R.
Drame, K.N.
Gracen, V.
Tongoona, P.B.
Dzidzienyo, D.
Nartey, E.
Greenberg, A.J.
Djiba, S.
Danquah, E.Y.
McCouch, S.R.
Bridging old and new: Diversity and evaluation of high iron-associated stress response of rice cultivated in West Africa
title Bridging old and new: Diversity and evaluation of high iron-associated stress response of rice cultivated in West Africa
title_full Bridging old and new: Diversity and evaluation of high iron-associated stress response of rice cultivated in West Africa
title_fullStr Bridging old and new: Diversity and evaluation of high iron-associated stress response of rice cultivated in West Africa
title_full_unstemmed Bridging old and new: Diversity and evaluation of high iron-associated stress response of rice cultivated in West Africa
title_short Bridging old and new: Diversity and evaluation of high iron-associated stress response of rice cultivated in West Africa
title_sort bridging old and new diversity and evaluation of high iron associated stress response of rice cultivated in west africa
topic rice
genetic diversity
research
url https://hdl.handle.net/10568/109110
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